Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry

14 pages, 4 figures, 4 tables

Detalles Bibliográficos
Autores: Gasol, Josep M., Morán, Xosé Anxelu G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/173718
Acceso en línea:http://hdl.handle.net/10261/173718
Access Level:acceso abierto
Palabra clave:Filtration
Bacterial activity
Flow cytometry
Picoalgae
Bacteria
Filters
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spelling Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometryGasol, Josep M.Morán, Xosé Anxelu G.FiltrationBacterial activityFlow cytometryPicoalgaeBacteriaFilters14 pages, 4 figures, 4 tablesWe used flow cytometry of autofluorescent and Syto13-stained marine bacteria and the uptake of tritiated leucine to assess the effects of different filter types on picoplankton abundance, community structure and bacterial activity in the filtrate. Coastal and oceanic samples from the NW and SW Mediterranean and the Atlantic coast of Galicia were size-fractionated using polycarbonate (PC), mixed cellulose esters (CE), aluminum oxide (IM) and glass fiber (GF) filters of 0.2 to 1.2 μm nominal pore size from different brands. Flow cytometry of Syto13-stained marine bacteria and autofluorescent photosynthetic prokaryotes was used to analyze picoplankton abundance, size structure and community composition before and after filtration. We combined this capability with the detection of the changes in cell-specific heterotrophic activity in the filtrates. We found that the CE filters retained picoplankton better than the PC filters. The PC filters did not discriminate prokaryotes according to size as much as the GF and the CE filters did. In our hands the IM filters were no better than the CE filters. Bacterial activity in the filtrates increased in the PC and in the CE filtrates and this stimulation of bacterial activity was more important in the less productive environments. We conclude that care must be taken when PC filters are used for generating bacteria-free water, and that the use of CE 0.22 μm filters is the best way of creating picoplankton-free water. However, the picoplankters that will go through the filters may encounter increased nutrient levels. © Inter-Research 1999The work reported here was supported by grants MAS3-CT95-0016 to C. Marrase. CYCYT AMB 94-1019 and 94-0853 to M. Estrada, DGICYT PB91-075, MAR95-1901-C03-03 and MAST3-CT9f-0154 to C. Pedros-Alio, and MAS3-CT96-0045 to C.M. DuartePeer ReviewedInter ResearchConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201919992019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/173718reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3354/ame016251Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1737182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
title Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
spellingShingle Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
Gasol, Josep M.
Filtration
Bacterial activity
Flow cytometry
Picoalgae
Bacteria
Filters
title_short Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
title_full Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
title_fullStr Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
title_full_unstemmed Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
title_sort Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
dc.creator.none.fl_str_mv Gasol, Josep M.
Morán, Xosé Anxelu G.
author Gasol, Josep M.
author_facet Gasol, Josep M.
Morán, Xosé Anxelu G.
author_role author
author2 Morán, Xosé Anxelu G.
author2_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Filtration
Bacterial activity
Flow cytometry
Picoalgae
Bacteria
Filters
topic Filtration
Bacterial activity
Flow cytometry
Picoalgae
Bacteria
Filters
description 14 pages, 4 figures, 4 tables
publishDate 1999
dc.date.none.fl_str_mv 1999
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/173718
url http://hdl.handle.net/10261/173718
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3354/ame016251
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Inter Research
publisher.none.fl_str_mv Inter Research
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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score 15.81155

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