N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures

Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five...

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Autores: Torres, Marta, Romero Bernárdez, Manuel, Prado Plana, Susana, Dubert Pérez, Javier, Tahrioui, Ali, Otero Casal, Ana María, Llamas, Inmaculada
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/41679
Acceso en línea:https://hdl.handle.net/10347/41679
Access Level:acceso abierto
Palabra clave:Quorum sensing
Aquaculture
Quorum quenching
Marine bacteria
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spelling N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval culturesTorres, MartaRomero Bernárdez, ManuelPrado Plana, SusanaDubert Pérez, JavierTahrioui, AliOtero Casal, Ana MaríaLlamas, InmaculadaQuorum sensingAquacultureQuorum quenchingMarine bacteriaQuorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-dl-homoserine lactone (C4-HSL), N-hexanoyl-dl-homoserine lactone (C6-HSL), N-octanoyl-dl-homoserine lactone (C8-HSL), N-decanoyl-dl-homoserine lactone (C10-HSL) and N-dodecanoyl-dl-homoserine lactone (C12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC–MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related.ElsevierUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía20132013-11-0720132013-11-07journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/41679reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available CGL2008-02399 ORIGEN, BIODIVERSIDAD Y BIOGEOGRAFIA DE LAS ESPECIES DEL GENERO HALOMONASMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available AGL2009-07656 Nueva Estrategia Para Controlar Las Enfermedades Infecciosas Bacterianas En Criaderos De Moluscos. Quorum Sensing Y Quorum Quenchingopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/416792026-06-15T12:47:27Z
dc.title.none.fl_str_mv N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
title N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
spellingShingle N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
Torres, Marta
Quorum sensing
Aquaculture
Quorum quenching
Marine bacteria
title_short N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
title_full N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
title_fullStr N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
title_full_unstemmed N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
title_sort N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures
dc.creator.none.fl_str_mv Torres, Marta
Romero Bernárdez, Manuel
Prado Plana, Susana
Dubert Pérez, Javier
Tahrioui, Ali
Otero Casal, Ana María
Llamas, Inmaculada
author Torres, Marta
author_facet Torres, Marta
Romero Bernárdez, Manuel
Prado Plana, Susana
Dubert Pérez, Javier
Tahrioui, Ali
Otero Casal, Ana María
Llamas, Inmaculada
author_role author
author2 Romero Bernárdez, Manuel
Prado Plana, Susana
Dubert Pérez, Javier
Tahrioui, Ali
Otero Casal, Ana María
Llamas, Inmaculada
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.subject.none.fl_str_mv Quorum sensing
Aquaculture
Quorum quenching
Marine bacteria
topic Quorum sensing
Aquaculture
Quorum quenching
Marine bacteria
description Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-dl-homoserine lactone (C4-HSL), N-hexanoyl-dl-homoserine lactone (C6-HSL), N-octanoyl-dl-homoserine lactone (C8-HSL), N-decanoyl-dl-homoserine lactone (C10-HSL) and N-dodecanoyl-dl-homoserine lactone (C12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC–MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-11-07
2013
2013-11-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/41679
url https://hdl.handle.net/10347/41679
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available CGL2008-02399 ORIGEN, BIODIVERSIDAD Y BIOGEOGRAFIA DE LAS ESPECIES DEL GENERO HALOMONAS
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 Not available AGL2009-07656 Nueva Estrategia Para Controlar Las Enfermedades Infecciosas Bacterianas En Criaderos De Moluscos. Quorum Sensing Y Quorum Quenching
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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