Evaluation of viral genome assembly and diversity estimation in deep metagenomes

[Background] Viruses have unique properties, small genome and regions of high similarity, whose effects on metagenomic assemblies have not been characterized so far. This study uses diverse in silico simulated viromes to evaluate how extensively genomes can be assembled using different sequencing pl...

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Detalhes bibliográficos
Autores: Aguirre de Cárcer, Daniel, Angly, Florent, Alcamí, Antonio
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/124422
Acesso em linha:http://hdl.handle.net/10261/124422
Access Level:acceso abierto
Palavra-chave:Assembly
Diversity
Metagenomics
Virome
Descrição
Resumo:[Background] Viruses have unique properties, small genome and regions of high similarity, whose effects on metagenomic assemblies have not been characterized so far. This study uses diverse in silico simulated viromes to evaluate how extensively genomes can be assembled using different sequencing platforms and assemblers. Further, it investigates the suitability of different methods to estimate viral diversity in metagenomes.