HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation
[EN] The gynoecium is critical for the reproduction of flowering plants as it contains the ovules and the tissues that foster pollen germination, growth, and guidance. These tissues, known as the reproductive tract (ReT), comprise the stigma, style, and transmitting tract (TT). The ReT and ovules or...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:dnet:riunet______::6b0a516ebfdf8c96bc387baf54e0ede3 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/234136 |
| Access Level: | acceso abierto |
| Palabra clave: | Gynoecium development Arabidopsis thaliana Carpel margin meristem Histone deacetylase 19 SHOOT MERISTEMLESS Ovule formation |
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HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| title |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| spellingShingle |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation Manrique-Urpí, Silvia|||0000-0001-6241-4143 Gynoecium development Arabidopsis thaliana Carpel margin meristem Histone deacetylase 19 SHOOT MERISTEMLESS Ovule formation |
| title_short |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| title_full |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| title_fullStr |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| title_full_unstemmed |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| title_sort |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract Differentiation |
| dc.creator.none.fl_str_mv |
Manrique-Urpí, Silvia|||0000-0001-6241-4143 Bombarely A Cavalleri, Alex Guazzotti, Andrea Villarino, Gonzalo H. Simonini, Sara Higashiyama, Tetsuya Grossniklaus, Ueli Mizzotti, Chiara Pereira, Ana Marta Coimbra, Silvia Sankaranarayanan, Subramanian Onelli, Elisabetta Masiero, Simona Franks, Robert G. |
| author |
Manrique-Urpí, Silvia|||0000-0001-6241-4143 |
| author_facet |
Manrique-Urpí, Silvia|||0000-0001-6241-4143 Bombarely A Cavalleri, Alex Guazzotti, Andrea Villarino, Gonzalo H. Simonini, Sara Higashiyama, Tetsuya Grossniklaus, Ueli Mizzotti, Chiara Pereira, Ana Marta Coimbra, Silvia Sankaranarayanan, Subramanian Onelli, Elisabetta Masiero, Simona Franks, Robert G. |
| author_role |
author |
| author2 |
Bombarely A Cavalleri, Alex Guazzotti, Andrea Villarino, Gonzalo H. Simonini, Sara Higashiyama, Tetsuya Grossniklaus, Ueli Mizzotti, Chiara Pereira, Ana Marta Coimbra, Silvia Sankaranarayanan, Subramanian Onelli, Elisabetta Masiero, Simona Franks, Robert G. |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Biotecnología Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural European Commission Swiss National Science Foundation European Molecular Biology Organization Fundação para a Ciência e a Tecnologia, Portugal Ministero dell'Istruzione, dell'Università e della Ricerca Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Gynoecium development Arabidopsis thaliana Carpel margin meristem Histone deacetylase 19 SHOOT MERISTEMLESS Ovule formation |
| topic |
Gynoecium development Arabidopsis thaliana Carpel margin meristem Histone deacetylase 19 SHOOT MERISTEMLESS Ovule formation |
| description |
[EN] The gynoecium is critical for the reproduction of flowering plants as it contains the ovules and the tissues that foster pollen germination, growth, and guidance. These tissues, known as the reproductive tract (ReT), comprise the stigma, style, and transmitting tract (TT). The ReT and ovules originate from the carpel margin meristem (CMM) within the pistil. SHOOT MERISTEMLESS (STM) is a key transcription factor for meristem formation and maintenance. In all above-ground meristems, including the CMM, local STM downregulation is required for organ formation. However, how this downregulation is achieved in the CMM is unknown. Here, we have studied the role of HISTONE DEACETYLASE 19 (HDA19) in Arabidopsis (Arabidopsis thaliana) during ovule and ReT differentiation based on the observation that the hda19-3 mutant displays a reduced ovule number and fails to differentiate the TT properly. Fluorescence-activated cell sorting coupled with RNA-sequencing revealed that in the CMM of hda19-3 mutants, genes promoting organ development are downregulated while meristematic markers, including STM, are upregulated. HDA19 was essential to downregulate STM in the CMM, thereby allowing ovule formation and TT differentiation. STM is ectopically expressed in hda19-3 at intermediate stages of pistil development, and its downregulation by RNA interference alleviated the hda19-3 phenotype. Chromatin immunoprecipitation assays indicated that STM is a direct target of HDA19 during pistil development and that the transcription factor SEEDSTICK is also required to regulate STM via histone acetylation. Thus, we identified factors required for the downregulation of STM in the CMM, which is necessary for organogenesis and tissue differentiation. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/234136 |
| url |
https://riunet.upv.es/handle/10251/234136 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 FP7 612640 The physiology and genetics of fruit formation: from genes to networks European Commission https://doi.org/10.13039/501100000780 H2020 690946 Sexual Plant Reproduction ¿ Seed formation Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 POCI-01-0145-FEDER-027839 Swiss National Science Foundation https://doi.org/10.13039/501100001711 31003A_179553 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
American Society of Plant Physiologists |
| publisher.none.fl_str_mv |
American Society of Plant Physiologists |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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1869412297634480128 |
| spelling |
HISTONE DEACETYLASE19 Controls Ovule Number Determination and Transmitting Tract DifferentiationManrique-Urpí, Silvia|||0000-0001-6241-4143Bombarely ACavalleri, AlexGuazzotti, AndreaVillarino, Gonzalo H.Simonini, SaraHigashiyama, TetsuyaGrossniklaus, UeliMizzotti, ChiaraPereira, Ana MartaCoimbra, SilviaSankaranarayanan, SubramanianOnelli, ElisabettaMasiero, SimonaFranks, Robert G.Gynoecium developmentArabidopsis thalianaCarpel margin meristemHistone deacetylase 19SHOOT MERISTEMLESSOvule formation[EN] The gynoecium is critical for the reproduction of flowering plants as it contains the ovules and the tissues that foster pollen germination, growth, and guidance. These tissues, known as the reproductive tract (ReT), comprise the stigma, style, and transmitting tract (TT). The ReT and ovules originate from the carpel margin meristem (CMM) within the pistil. SHOOT MERISTEMLESS (STM) is a key transcription factor for meristem formation and maintenance. In all above-ground meristems, including the CMM, local STM downregulation is required for organ formation. However, how this downregulation is achieved in the CMM is unknown. Here, we have studied the role of HISTONE DEACETYLASE 19 (HDA19) in Arabidopsis (Arabidopsis thaliana) during ovule and ReT differentiation based on the observation that the hda19-3 mutant displays a reduced ovule number and fails to differentiate the TT properly. Fluorescence-activated cell sorting coupled with RNA-sequencing revealed that in the CMM of hda19-3 mutants, genes promoting organ development are downregulated while meristematic markers, including STM, are upregulated. HDA19 was essential to downregulate STM in the CMM, thereby allowing ovule formation and TT differentiation. STM is ectopically expressed in hda19-3 at intermediate stages of pistil development, and its downregulation by RNA interference alleviated the hda19-3 phenotype. Chromatin immunoprecipitation assays indicated that STM is a direct target of HDA19 during pistil development and that the transcription factor SEEDSTICK is also required to regulate STM via histone acetylation. Thus, we identified factors required for the downregulation of STM in the CMM, which is necessary for organogenesis and tissue differentiation.S.Man., A.C., and L.C. were funded by MIUR (PRIN2017). A.G. and L.C. were funded by H2020 Excellent Science (SEXSEED ID: 690946). S.Man. was funded by an EMBO short-term fellowship (2015). S.Man., S.Mas., and C.M. were funded by FP7 People: Marie-Curie Actions International Research Staff Exchange Scheme (FRUITLOOK ID: 612640). S.C. was funded by Fundação para a Ciência e a Tecnologia (Project POCI-01-0145-FEDER-027839). U.G. was funded by the Swiss National Science Foundation (grant 31003A_179553).American Society of Plant PhysiologistsDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionSwiss National Science FoundationEuropean Molecular Biology OrganizationFundação para a Ciência e a Tecnologia, PortugalMinistero dell'Istruzione, dell'Università e della RicercaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/234136reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 FP7 612640 The physiology and genetics of fruit formation: from genes to networksEuropean Commission https://doi.org/10.13039/501100000780 H2020 690946 Sexual Plant Reproduction ¿ Seed formationFundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 POCI-01-0145-FEDER-027839Swiss National Science Foundation https://doi.org/10.13039/501100001711 31003A_179553open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:riunet______::6b0a516ebfdf8c96bc387baf54e0ede32026-06-13T07:49:27Z |
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15,812429 |