Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis

Growing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low-density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a cohort of patient...

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Autores: Fernandez-Bravo, Sergio, Canyelles, Marina|||0000-0002-0278-5337, Martín-Blázquez, Ariadna, Borràs, Carla|||0000-0002-0998-7855, Nuñez-Borque, Emilio, Palacio-García, Lucia, Rodríguez Del Rio, Pablo, Betancor, Diana|||0000-0003-2765-3581, Gómez-López, Alicia, Sabaté Brescó, Marina, Laguna, José Julio, Mendez-Barbero, Nerea|||0000-0003-1620-3067, Rotllan, Noemi|||0000-0002-0587-8045, Escolà-Gil, Joan Carles|||0000-0001-9021-2485, Esteban, Vanesa|||0000-0002-7500-6277
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:309009
Acceso en línea:https://ddd.uab.cat/record/309009
https://dx.doi.org/urn:doi:10.1016/j.jaci.2024.03.031
Access Level:acceso abierto
Palabra clave:Anaphylaxis
High-density lipoprotein cholesterol
Macrophage cholesterol efflux
Vascular permeability
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spelling Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxisFernandez-Bravo, SergioCanyelles, Marina|||0000-0002-0278-5337Martín-Blázquez, AriadnaBorràs, Carla|||0000-0002-0998-7855Nuñez-Borque, EmilioPalacio-García, LuciaRodríguez Del Rio, PabloBetancor, Diana|||0000-0003-2765-3581Gómez-López, AliciaSabaté Brescó, MarinaLaguna, José JulioMendez-Barbero, Nerea|||0000-0003-1620-3067Rotllan, Noemi|||0000-0002-0587-8045Escolà-Gil, Joan Carles|||0000-0001-9021-2485Esteban, Vanesa|||0000-0002-7500-6277AnaphylaxisHigh-density lipoprotein cholesterolMacrophage cholesterol effluxVascular permeabilityGrowing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low-density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a cohort of patients presenting with hypersensitivity reactions. We further assessed the function of high-density lipoprotein particles as well as their involvement in the molecular mechanisms of anaphylaxis. Lipid profile determination was performed in paired (acute and baseline) serum samples from 153 patients. Thirty-eight experienced a non-anaphylactic reaction and 115 had an anaphylactic reaction (88 moderate and 27 severe). Lecithin cholesterol acyl transferase activity was assessed in patient sera, and we also evaluated macrophage cholesterol efflux in response to the serum samples. Last, the effect of anaphylactic-derived high-density lipoprotein (HDL) particles on the endothelial barrier was studied. Detailed methods are provided in the Methods section in this article's Online Repository available at www.jacionline.org. Serum samples from severe anaphylactic reactions show statistically significant low levels of HDL cholesterol, low-density lipoprotein cholesterol, and apolipoproteins A1 and B, which points to their possible role as biomarkers. Specifically, HDL particles play a protective role in cardiovascular diseases. Using functional human serum cell assays, we observed impaired capacity of apolipoprotein B-depleted serum to induce macrophage cholesterol efflux in severe anaphylactic reactions. In addition, purified HDL particles from human anaphylactic sera failed to stabilize and maintain the endothelial barrier. These results encourage further research on HDL functions in severe anaphylaxis, which may lead to new diagnostic and therapeutic strategies.Universitat Autònoma de Barcelona 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/309009https://dx.doi.org/urn:doi:10.1016/j.jaci.2024.03.031reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/00158Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/01126Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/00969Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0013Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0026Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0033open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3090092026-06-06T12:50:31Z
dc.title.none.fl_str_mv Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
spellingShingle Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
Fernandez-Bravo, Sergio
Anaphylaxis
High-density lipoprotein cholesterol
Macrophage cholesterol efflux
Vascular permeability
title_short Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_full Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_fullStr Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_full_unstemmed Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
title_sort Impaired high-density lipoprotein function and endothelial barrier stability in severe anaphylaxis
dc.creator.none.fl_str_mv Fernandez-Bravo, Sergio
Canyelles, Marina|||0000-0002-0278-5337
Martín-Blázquez, Ariadna
Borràs, Carla|||0000-0002-0998-7855
Nuñez-Borque, Emilio
Palacio-García, Lucia
Rodríguez Del Rio, Pablo
Betancor, Diana|||0000-0003-2765-3581
Gómez-López, Alicia
Sabaté Brescó, Marina
Laguna, José Julio
Mendez-Barbero, Nerea|||0000-0003-1620-3067
Rotllan, Noemi|||0000-0002-0587-8045
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Esteban, Vanesa|||0000-0002-7500-6277
author Fernandez-Bravo, Sergio
author_facet Fernandez-Bravo, Sergio
Canyelles, Marina|||0000-0002-0278-5337
Martín-Blázquez, Ariadna
Borràs, Carla|||0000-0002-0998-7855
Nuñez-Borque, Emilio
Palacio-García, Lucia
Rodríguez Del Rio, Pablo
Betancor, Diana|||0000-0003-2765-3581
Gómez-López, Alicia
Sabaté Brescó, Marina
Laguna, José Julio
Mendez-Barbero, Nerea|||0000-0003-1620-3067
Rotllan, Noemi|||0000-0002-0587-8045
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Esteban, Vanesa|||0000-0002-7500-6277
author_role author
author2 Canyelles, Marina|||0000-0002-0278-5337
Martín-Blázquez, Ariadna
Borràs, Carla|||0000-0002-0998-7855
Nuñez-Borque, Emilio
Palacio-García, Lucia
Rodríguez Del Rio, Pablo
Betancor, Diana|||0000-0003-2765-3581
Gómez-López, Alicia
Sabaté Brescó, Marina
Laguna, José Julio
Mendez-Barbero, Nerea|||0000-0003-1620-3067
Rotllan, Noemi|||0000-0002-0587-8045
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Esteban, Vanesa|||0000-0002-7500-6277
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Anaphylaxis
High-density lipoprotein cholesterol
Macrophage cholesterol efflux
Vascular permeability
topic Anaphylaxis
High-density lipoprotein cholesterol
Macrophage cholesterol efflux
Vascular permeability
description Growing evidence demonstrates the importance of high- and low-density lipoprotein cholesterol in certain immune and allergy-mediated diseases. Objective: This study aimed to evaluate levels of high- and low-density lipoprotein cholesterol and apolipoproteins A1 and B in sera from a cohort of patients presenting with hypersensitivity reactions. We further assessed the function of high-density lipoprotein particles as well as their involvement in the molecular mechanisms of anaphylaxis. Lipid profile determination was performed in paired (acute and baseline) serum samples from 153 patients. Thirty-eight experienced a non-anaphylactic reaction and 115 had an anaphylactic reaction (88 moderate and 27 severe). Lecithin cholesterol acyl transferase activity was assessed in patient sera, and we also evaluated macrophage cholesterol efflux in response to the serum samples. Last, the effect of anaphylactic-derived high-density lipoprotein (HDL) particles on the endothelial barrier was studied. Detailed methods are provided in the Methods section in this article's Online Repository available at www.jacionline.org. Serum samples from severe anaphylactic reactions show statistically significant low levels of HDL cholesterol, low-density lipoprotein cholesterol, and apolipoproteins A1 and B, which points to their possible role as biomarkers. Specifically, HDL particles play a protective role in cardiovascular diseases. Using functional human serum cell assays, we observed impaired capacity of apolipoprotein B-depleted serum to induce macrophage cholesterol efflux in severe anaphylactic reactions. In addition, purified HDL particles from human anaphylactic sera failed to stabilize and maintain the endothelial barrier. These results encourage further research on HDL functions in severe anaphylaxis, which may lead to new diagnostic and therapeutic strategies.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/309009
https://dx.doi.org/urn:doi:10.1016/j.jaci.2024.03.031
url https://ddd.uab.cat/record/309009
https://dx.doi.org/urn:doi:10.1016/j.jaci.2024.03.031
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/00158
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/01126
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI21/00969
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0013
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0026
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0006/0033
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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