RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis
[Background] Enteromyxosis caused by the intestinal myxozoan parasite Enteromyxum scophthalmi is a serious threat for turbot (Scophthalmus maximus, L.) aquaculture, causing severe catarrhal enteritis leading to a cachectic syndrome, with no therapeutic options available. There are still many aspects...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/125855 |
| Acceso en línea: | http://hdl.handle.net/10261/125855 |
| Access Level: | acceso abierto |
| Palabra clave: | RNA-seq Transcriptomes Turbot Enteromyxum scophthalmi Enteromyxosis Immune response Apoptosis Erythropoiesis Cytoskeleton Digestive function |
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oai:digital.csic.es:10261/125855 |
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RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosisRobledo, DiegoRonza, PaoloHarrison, Peter W.Losada, Ana PaulaBermúdez, RobertoPardo, Belén G.Redondo, Mª JoséSitjà-Bobadilla, AriadnaQuiroga, María IsabelMartínez, PaulinoRNA-seqTranscriptomesTurbotEnteromyxum scophthalmiEnteromyxosisImmune responseApoptosisErythropoiesisCytoskeletonDigestive function[Background] Enteromyxosis caused by the intestinal myxozoan parasite Enteromyxum scophthalmi is a serious threat for turbot (Scophthalmus maximus, L.) aquaculture, causing severe catarrhal enteritis leading to a cachectic syndrome, with no therapeutic options available. There are still many aspects of host-parasite interaction and disease pathogenesis that are yet to be elucidated, and to date, no analysis of the transcriptomic changes induced by E. scophthalmi in turbot organs has been conducted. In this study, RNA-seq technology was applied to head kidney, spleen and pyloric caeca of severely infected turbot with the aim of furthering our understanding of the pathogenetic mechanisms and turbot immune response against enteromyxosis.[Results] A huge amount of information was generated with more than 23,000 identified genes in the three organs, amongst which 4,762 were differently expressed (DE) between infected and control fish. Associate gene functions were studied based on gene ontology terms and available literature, and the most interesting DE genes were classified into five categories: 1) immune and defence response; 2) apoptosis and cell proliferation; 3) iron metabolism and erythropoiesis; 4) cytoskeleton and extracellular matrix and 5) metabolism and digestive function. The analysis of down-regulated genes of the first category revealed evidences of a connexion failure between innate and adaptive immune response, especially represented by a high number of DE interferon-related genes in the three organs. Furthermore, we found an intense activation of local immune response at intestinal level that appeared exacerbated, whereas in kidney and spleen genes involved in adaptive immune response were mainly down-regulated. The apoptotic machinery was only clearly activated in pyloric caeca, while kidney and spleen showed a marked depression of genes related to erythropoiesis, probably related to disorders in iron homeostasis. The genetic signature of the causes and consequences of cachexia was also demonstrated by the down-regulation of the genes encoding structural proteins and those involved in the digestive metabolism.[Conclusions] This transcriptomic study has enabled us to gain a better understanding of the pathogenesis of enteromyxosis and identify a large number of DE target genes that bring us closer to the development of strategies designed to effectively combat this pathogen.PR was supported by a predoctoral contract from the Universidad de Santiago de Compostela. This work was funded by the Spanish Ministry of Science and Innovation through the projects AGL2009-13282-C02-01 and -02.BioMed CentralUniversidad de Santiago de CompostelaMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520142015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/125855reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/1471-2164-15-1149Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1258552026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| title |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| spellingShingle |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis Robledo, Diego RNA-seq Transcriptomes Turbot Enteromyxum scophthalmi Enteromyxosis Immune response Apoptosis Erythropoiesis Cytoskeleton Digestive function |
| title_short |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| title_full |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| title_fullStr |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| title_full_unstemmed |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| title_sort |
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis |
| dc.creator.none.fl_str_mv |
Robledo, Diego Ronza, Paolo Harrison, Peter W. Losada, Ana Paula Bermúdez, Roberto Pardo, Belén G. Redondo, Mª José Sitjà-Bobadilla, Ariadna Quiroga, María Isabel Martínez, Paulino |
| author |
Robledo, Diego |
| author_facet |
Robledo, Diego Ronza, Paolo Harrison, Peter W. Losada, Ana Paula Bermúdez, Roberto Pardo, Belén G. Redondo, Mª José Sitjà-Bobadilla, Ariadna Quiroga, María Isabel Martínez, Paulino |
| author_role |
author |
| author2 |
Ronza, Paolo Harrison, Peter W. Losada, Ana Paula Bermúdez, Roberto Pardo, Belén G. Redondo, Mª José Sitjà-Bobadilla, Ariadna Quiroga, María Isabel Martínez, Paulino |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Santiago de Compostela Ministerio de Ciencia e Innovación (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
RNA-seq Transcriptomes Turbot Enteromyxum scophthalmi Enteromyxosis Immune response Apoptosis Erythropoiesis Cytoskeleton Digestive function |
| topic |
RNA-seq Transcriptomes Turbot Enteromyxum scophthalmi Enteromyxosis Immune response Apoptosis Erythropoiesis Cytoskeleton Digestive function |
| description |
[Background] Enteromyxosis caused by the intestinal myxozoan parasite Enteromyxum scophthalmi is a serious threat for turbot (Scophthalmus maximus, L.) aquaculture, causing severe catarrhal enteritis leading to a cachectic syndrome, with no therapeutic options available. There are still many aspects of host-parasite interaction and disease pathogenesis that are yet to be elucidated, and to date, no analysis of the transcriptomic changes induced by E. scophthalmi in turbot organs has been conducted. In this study, RNA-seq technology was applied to head kidney, spleen and pyloric caeca of severely infected turbot with the aim of furthering our understanding of the pathogenetic mechanisms and turbot immune response against enteromyxosis. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2015 2015 2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/125855 |
| url |
http://hdl.handle.net/10261/125855 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1186/1471-2164-15-1149 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
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1869412295185006592 |
| score |
15,81155 |