Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for chall...

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Detalles Bibliográficos
Autores: Iglesias-Caballero, M., Camarero-Serrano, S., Varona, S., Mas, V., Calvo, C., García, M.L., García Costa, Juan, Vázquez-Morón, S., Monzón, S., Campoy, A., Cuesta, I., Pozo, F., Casas, I.
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/21536
Acceso en línea:https://portalcientifico.sergas.gal//documentos/65911dbbae63c86e421b9c34
http://hdl.handle.net/20.500.11940/21536
Access Level:acceso abierto
Palabra clave:Humans
Respiratory Syncytial Virus Infections
Viral Fusion Proteins
Respiratory Syncytial Virus Vaccines
Respiratory Syncytial Virus, Human
Genomics
Whole Genome Sequencing
Antibodies, Viral
AS Ourense
CHUO
Descripción
Sumario:To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.

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