Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis

Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were...

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Autores: Rebollada Merino, Agustín Miguel, Martínez Alares, Irene, Duque, Clara, García-Seco Romero, María Teresa, Escacena, Cristina, Domínguez Rodríguez, Lucas José, Rodríguez Bertos, Antonio Manuel, García Benzaquén, Nerea
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102528
Acceso en línea:https://hdl.handle.net/20.500.14352/102528
Access Level:acceso abierto
Palabra clave:636.09
Veterinaria
3109 Ciencias Veterinarias
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spelling Detection of Brucella in Dermacentor Ticks of Wild Boar with BrucellosisRebollada Merino, Agustín MiguelMartínez Alares, IreneDuque, ClaraGarcía-Seco Romero, María TeresaEscacena, CristinaDomínguez Rodríguez, Lucas JoséRodríguez Bertos, Antonio ManuelGarcía Benzaquén, Nerea636.09Veterinaria3109 Ciencias VeterinariasBrucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 70.9%–81.8%), red deer (Cervus elaphus, n = 40; 17.4%, 95% CI 13.1%–22.9%), mouflon (Ovis orientalis musimon, n = 7; 3.06%, 95% CI 1.49%–6.17%), and fallow deer (Dama dama, n = 6; 2.62%, 95% CI 1.21%–5.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%–7.30%) tick pools (9/229) from 16.6% hunting reserves (7/41) screened yielded a positive PCR result for Brucella. All positive ticks were Dermacentor (Dermacentor marginatus or Dermacentor reticulatus) collected from wild boar. Ticks collected from wild boars were positive to Brucella in a relative percentage of 5.10% (95% CI = 1.61–11.4) in 2018 and of 7.59% (95% CI = 2.79–15.6) in 2021 (6-year prevalence of 5.17%, 9/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for Brucella in ticks. To conclude, Brucella spp. DNA can be detected in Dermacentor ticks parasitizing wild boars living in hunting reserves harboring Brucella spp.-seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife.HindawiDaniel DiazUniversidad Complutense de Madrid20242024-01-0120242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102528reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098658-B-C22 EL SISTEMA DE DOS COMPONENTES WZM%2FWZT DE BRUCELLA: ESTUDIOS MOLECULARES, INTERACCIONES PATOGENO-HOSPEDADOR EN OVINO Y APLICACIONES EN B. SUISopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1025282026-06-02T12:44:21Z
dc.title.none.fl_str_mv Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
title Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
spellingShingle Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
Rebollada Merino, Agustín Miguel
636.09
Veterinaria
3109 Ciencias Veterinarias
title_short Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
title_full Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
title_fullStr Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
title_full_unstemmed Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
title_sort Detection of Brucella in Dermacentor Ticks of Wild Boar with Brucellosis
dc.creator.none.fl_str_mv Rebollada Merino, Agustín Miguel
Martínez Alares, Irene
Duque, Clara
García-Seco Romero, María Teresa
Escacena, Cristina
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
García Benzaquén, Nerea
author Rebollada Merino, Agustín Miguel
author_facet Rebollada Merino, Agustín Miguel
Martínez Alares, Irene
Duque, Clara
García-Seco Romero, María Teresa
Escacena, Cristina
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
García Benzaquén, Nerea
author_role author
author2 Martínez Alares, Irene
Duque, Clara
García-Seco Romero, María Teresa
Escacena, Cristina
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
García Benzaquén, Nerea
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Daniel Diaz
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Veterinaria
3109 Ciencias Veterinarias
description Brucellosis is a sanitary and economically relevant disease affecting humans, livestock, and wildlife. Ticks have been suggested as vectors, long-term carriers, and amplifiers of Brucella. In this study, ticks from wildlife ungulate hosts living in hunting reserves of a central region of Spain were collected during a 6-year period, pooled, and screened for Brucella spp. by PCR. Aiming to correlate Brucella spp. DNA presence in ticks with Brucella spp. infections in wildlife ungulate hosts, liver samples from deceased wildlife ungulates coming from the hunting reserves showing a positive result for Brucella in ticks were tested using a commercial ELISA. In total, 229 tick pools from wild boar (Sus scrofa, n = 176; 76.8%, 95% CI 70.9%–81.8%), red deer (Cervus elaphus, n = 40; 17.4%, 95% CI 13.1%–22.9%), mouflon (Ovis orientalis musimon, n = 7; 3.06%, 95% CI 1.49%–6.17%), and fallow deer (Dama dama, n = 6; 2.62%, 95% CI 1.21%–5.60%) were analyzed. PCR results showed that 3.93% (95% CI 2.08%–7.30%) tick pools (9/229) from 16.6% hunting reserves (7/41) screened yielded a positive PCR result for Brucella. All positive ticks were Dermacentor (Dermacentor marginatus or Dermacentor reticulatus) collected from wild boar. Ticks collected from wild boars were positive to Brucella in a relative percentage of 5.10% (95% CI = 1.61–11.4) in 2018 and of 7.59% (95% CI = 2.79–15.6) in 2021 (6-year prevalence of 5.17%, 9/176). ELISA showed positive results in three wild boars coming from two out of seven hunting reserves (28.5%) with a positive PCR for Brucella in ticks. To conclude, Brucella spp. DNA can be detected in Dermacentor ticks parasitizing wild boars living in hunting reserves harboring Brucella spp.-seropositive wild boars. This study provides evidence that the contribution of arthropod vectors should be considered in the epidemiology of brucellosis in wildlife.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102528
url https://hdl.handle.net/20.500.14352/102528
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098658-B-C22 EL SISTEMA DE DOS COMPONENTES WZM%2FWZT DE BRUCELLA: ESTUDIOS MOLECULARES, INTERACCIONES PATOGENO-HOSPEDADOR EN OVINO Y APLICACIONES EN B. SUIS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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