GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation

Background: We report on the functional screening and identification of an active quorum quenching (QQ) gene in the Komagataeibacter europaeus strain CECT 8546, which is a member of the acetic acid bacteria (AAB). Results: Using a previously published screening protocol (Schipper et al., in Appl Env...

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Autores: Valera, María José, Mas Barón, Albert, Streit, Wolfgang R., Mateo Alesanco, Estibaliz
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/32327
Acceso en línea:http://hdl.handle.net/10810/32327
Access Level:acceso abierto
Palabra clave:homoserine lactone
acetic acid bacteria
vinegar
quorum quenching
genomic fosmid library
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spelling GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose FormationValera, María JoséMas Barón, AlbertStreit, Wolfgang R.Mateo Alesanco, Estibalizhomoserine lactoneacetic acid bacteriavinegarquorum quenchinggenomic fosmid libraryBackground: We report on the functional screening and identification of an active quorum quenching (QQ) gene in the Komagataeibacter europaeus strain CECT 8546, which is a member of the acetic acid bacteria (AAB). Results: Using a previously published screening protocol (Schipper et al., in Appl Environ Microbiol 75: 224-233, 2009. doi: 10.1128/AEM.01389-08) for QQ genes, we identified a single gene, designated gqqA, that interfered strongly with bacterial quorum sensing (QS) in various reporter strains. It encodes for a 281-amino acid protein with a molecular mass of 30 kDa. Although the GqqA protein is similar to predicted prephenate dehydratases, it does not complement Escherichia coli mutants of the pheA gene, thus indicating a potentially different function. Recombinant GqqA protein attenuated QS-dependent pyocyanin production and swarming motility in the Pseudomonas aeruginosa strain PAO1. Moreover, GqqA quenched the QS response of the Agrobacterium tumefaciens NTL4 and the Chromobacterium violaceum CV026 reporter strains. Interestingly, the addition of recombinant GqqA protein to growing cultures of the Komagataeibacter europaeus strain CECT 8546 altered the cellulose production phenotype of CECT 8546 and other AAB strains. In the presence of GqqA protein, cells were planktonic, and no visible cellulose biofilms formed. The addition of low levels of N-acylhomoserine lactones maintained the biofilm formation phenotype. Conclusions: Our data provide evidence for an interconnection between QS and AAB cellulose biofilm formation as well as QQ activity of the GqqA protein.This work was supported by BMBF within the ChemBiofilm Network at the University of Hamburg and by the Grant GL2010-22152-C03-02 from the Spanish Ministry of Science and Innovation. Collaboration between the two groups was possible through the fellowship AP2009-0843 from the Spanish Ministry of Education, Culture and Sports for M.J. Valera.Biomed Central201920192016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/32327reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL2010-22152-C03-02/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0482-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/oai:addi.ehu.eus:10810/323272026-06-18T09:23:17Z
dc.title.none.fl_str_mv GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
title GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
spellingShingle GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
Valera, María José
homoserine lactone
acetic acid bacteria
vinegar
quorum quenching
genomic fosmid library
title_short GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
title_full GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
title_fullStr GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
title_full_unstemmed GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
title_sort GqqA, a Novel Protein in Komagataeibacter Europaeus Involved in Bacterial Quorum Quenching and Cellulose Formation
dc.creator.none.fl_str_mv Valera, María José
Mas Barón, Albert
Streit, Wolfgang R.
Mateo Alesanco, Estibaliz
author Valera, María José
author_facet Valera, María José
Mas Barón, Albert
Streit, Wolfgang R.
Mateo Alesanco, Estibaliz
author_role author
author2 Mas Barón, Albert
Streit, Wolfgang R.
Mateo Alesanco, Estibaliz
author2_role author
author
author
dc.subject.none.fl_str_mv homoserine lactone
acetic acid bacteria
vinegar
quorum quenching
genomic fosmid library
topic homoserine lactone
acetic acid bacteria
vinegar
quorum quenching
genomic fosmid library
description Background: We report on the functional screening and identification of an active quorum quenching (QQ) gene in the Komagataeibacter europaeus strain CECT 8546, which is a member of the acetic acid bacteria (AAB). Results: Using a previously published screening protocol (Schipper et al., in Appl Environ Microbiol 75: 224-233, 2009. doi: 10.1128/AEM.01389-08) for QQ genes, we identified a single gene, designated gqqA, that interfered strongly with bacterial quorum sensing (QS) in various reporter strains. It encodes for a 281-amino acid protein with a molecular mass of 30 kDa. Although the GqqA protein is similar to predicted prephenate dehydratases, it does not complement Escherichia coli mutants of the pheA gene, thus indicating a potentially different function. Recombinant GqqA protein attenuated QS-dependent pyocyanin production and swarming motility in the Pseudomonas aeruginosa strain PAO1. Moreover, GqqA quenched the QS response of the Agrobacterium tumefaciens NTL4 and the Chromobacterium violaceum CV026 reporter strains. Interestingly, the addition of recombinant GqqA protein to growing cultures of the Komagataeibacter europaeus strain CECT 8546 altered the cellulose production phenotype of CECT 8546 and other AAB strains. In the presence of GqqA protein, cells were planktonic, and no visible cellulose biofilms formed. The addition of low levels of N-acylhomoserine lactones maintained the biofilm formation phenotype. Conclusions: Our data provide evidence for an interconnection between QS and AAB cellulose biofilm formation as well as QQ activity of the GqqA protein.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/32327
url http://hdl.handle.net/10810/32327
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL2010-22152-C03-02/
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0482-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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