Antibiotic resistance along an urban river impacted by treated wastewaters

Urban rivers are impacted ecosystems which may play an important role as reservoirs for antibiotic-resistant (AR) bacteria. The main objective of this study was to describe the prevalence of antibiotic resistance along a sewage-polluted urban river. Seven sites along the Zenne River (Belgium) were s...

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Detalhes bibliográficos
Autores: Proia, Lorenzo, Anzil, Adriana, Subirats Medina, Jessica, Borrego i Moré, Carles, Farré, Marinella, Llorca, Marta, Balcázar, José Luis, Servais, Pierre
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/25467
Acesso em linha:http://hdl.handle.net/10256/25467
Access Level:acceso abierto
Palavra-chave:Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Eliminació
Sewage disposal
Contaminants emergents en l'aigua
Emerging contaminants in water
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spelling Antibiotic resistance along an urban river impacted by treated wastewatersProia, LorenzoAnzil, AdrianaSubirats Medina, JessicaBorrego i Moré, CarlesFarré, MarinellaLlorca, MartaBalcázar, José LuisServais, PierreMedicaments -- Aspectes ambientalsDrugs -- Environmental aspectsAigües residuals -- EliminacióSewage disposalContaminants emergents en l'aiguaEmerging contaminants in waterUrban rivers are impacted ecosystems which may play an important role as reservoirs for antibiotic-resistant (AR) bacteria. The main objective of this study was to describe the prevalence of antibiotic resistance along a sewage-polluted urban river. Seven sites along the Zenne River (Belgium) were selected to study the prevalence of AR Escherichia coli and freshwater bacteria over a 1-year period. Culture-dependent methods were used to estimate E. coli and heterotrophic bacteria resistant to amoxicillin, sulfamethoxazole, nalidixic acid and tetracycline. The concentrations of these four antibiotics have been quantified in the studied river. The antibiotic resistance genes (ARGs), sul1, sul2, tetW, tetO, blaTEM and qnrS were also quantified in both particle-attached (PAB) and free-living (FLB) bacteria. Our results showed an effect of treated wastewaters release on the spread of antibiotic resistance along the river. Although an increase in the abundance of both AR E. coli and resistant heterotrophic bacteria was observed from upstream to downstream sites, the differences were only significant for AR E. coli. A significant positive regression was also found between AR E. coli and resistant heterotrophic bacteria. The concentration of ARGs increased from upstream to downstream sites for both particle-attached (PAB) and free-living bacteria (FLB). Particularly, a significant increase in the abundance of four among six ARGs analyzed was observed after crossing urban area. Although concentrations of tetracycline significantly correlated with tetracycline resistance genes, the antibiotic levels were likely too low to explain this correlation. The analysis of ARGs in different fractions revealed a significantly higher abundance in PAB compared to FLB for tetO and sul2 genes. This study demonstrated that urban activities may increase the spread of antibiotic resistance even in an already impacted river6Elsevierinfo2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/25467http://hdl.handle.net/10256/25467© Science of the Total Environment, 2018, vol. 628-629, p. 453-466Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2018.02.083info:eu-repo/semantics/altIdentifier/issn/0048-9697info:eu-repo/semantics/altIdentifier/eissn/1879-1026Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/254672026-05-29T05:05:01Z
dc.title.none.fl_str_mv Antibiotic resistance along an urban river impacted by treated wastewaters
title Antibiotic resistance along an urban river impacted by treated wastewaters
spellingShingle Antibiotic resistance along an urban river impacted by treated wastewaters
Proia, Lorenzo
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Eliminació
Sewage disposal
Contaminants emergents en l'aigua
Emerging contaminants in water
title_short Antibiotic resistance along an urban river impacted by treated wastewaters
title_full Antibiotic resistance along an urban river impacted by treated wastewaters
title_fullStr Antibiotic resistance along an urban river impacted by treated wastewaters
title_full_unstemmed Antibiotic resistance along an urban river impacted by treated wastewaters
title_sort Antibiotic resistance along an urban river impacted by treated wastewaters
dc.creator.none.fl_str_mv Proia, Lorenzo
Anzil, Adriana
Subirats Medina, Jessica
Borrego i Moré, Carles
Farré, Marinella
Llorca, Marta
Balcázar, José Luis
Servais, Pierre
author Proia, Lorenzo
author_facet Proia, Lorenzo
Anzil, Adriana
Subirats Medina, Jessica
Borrego i Moré, Carles
Farré, Marinella
Llorca, Marta
Balcázar, José Luis
Servais, Pierre
author_role author
author2 Anzil, Adriana
Subirats Medina, Jessica
Borrego i Moré, Carles
Farré, Marinella
Llorca, Marta
Balcázar, José Luis
Servais, Pierre
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Eliminació
Sewage disposal
Contaminants emergents en l'aigua
Emerging contaminants in water
topic Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Aigües residuals -- Eliminació
Sewage disposal
Contaminants emergents en l'aigua
Emerging contaminants in water
description Urban rivers are impacted ecosystems which may play an important role as reservoirs for antibiotic-resistant (AR) bacteria. The main objective of this study was to describe the prevalence of antibiotic resistance along a sewage-polluted urban river. Seven sites along the Zenne River (Belgium) were selected to study the prevalence of AR Escherichia coli and freshwater bacteria over a 1-year period. Culture-dependent methods were used to estimate E. coli and heterotrophic bacteria resistant to amoxicillin, sulfamethoxazole, nalidixic acid and tetracycline. The concentrations of these four antibiotics have been quantified in the studied river. The antibiotic resistance genes (ARGs), sul1, sul2, tetW, tetO, blaTEM and qnrS were also quantified in both particle-attached (PAB) and free-living (FLB) bacteria. Our results showed an effect of treated wastewaters release on the spread of antibiotic resistance along the river. Although an increase in the abundance of both AR E. coli and resistant heterotrophic bacteria was observed from upstream to downstream sites, the differences were only significant for AR E. coli. A significant positive regression was also found between AR E. coli and resistant heterotrophic bacteria. The concentration of ARGs increased from upstream to downstream sites for both particle-attached (PAB) and free-living bacteria (FLB). Particularly, a significant increase in the abundance of four among six ARGs analyzed was observed after crossing urban area. Although concentrations of tetracycline significantly correlated with tetracycline resistance genes, the antibiotic levels were likely too low to explain this correlation. The analysis of ARGs in different fractions revealed a significantly higher abundance in PAB compared to FLB for tetO and sul2 genes. This study demonstrated that urban activities may increase the spread of antibiotic resistance even in an already impacted river
publishDate 2018
dc.date.none.fl_str_mv 2018
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/25467
http://hdl.handle.net/10256/25467
url http://hdl.handle.net/10256/25467
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2018.02.083
info:eu-repo/semantics/altIdentifier/issn/0048-9697
info:eu-repo/semantics/altIdentifier/eissn/1879-1026
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Science of the Total Environment, 2018, vol. 628-629, p. 453-466
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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