Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs

African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on...

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Autores: Pérez-Núñez, Daniel, Sunwoo, Sun-Young, Sánchez, Elena G., Haley, Nicholas, García-Belmonte, Raquel, Nogal, Marisa, Morozov, Igor, Madden, Daniel, Gaudreault, Natasha N., Mur, Lina, Shivana, Vinay, Richt, Jüergen A., Revilla Novella, Yolanda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/214533
Acceso en línea:http://hdl.handle.net/10261/214533
Access Level:acceso abierto
Palabra clave:African swine fever virus
Heterologous DNA-protein immunization
Plasmid-expressed antigen
Recombinant protein
Immune response
Immunopathology
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spelling Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigsPérez-Núñez, DanielSunwoo, Sun-YoungSánchez, Elena G.Haley, NicholasGarcía-Belmonte, RaquelNogal, MarisaMorozov, IgorMadden, DanielGaudreault, Natasha N.Mur, LinaShivana, VinayRicht, Jüergen A.Revilla Novella, YolandaAfrican swine fever virusHeterologous DNA-protein immunizationPlasmid-expressed antigenRecombinant proteinImmune responseImmunopathologyAfrican swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.U.S. Department of Homeland Security under Grant Award Number DHS-2010-ST-061-AG0001 for the Center of Excellence for Emerging and Zoonotic Animal Disease (CEEZAD) and the State of Kansas National Bio and Agro-Defense Facility (NBAF)US Department of Homeland SecurityCenter of Excellence for Emerging and Zoonotic Animal Diseases (US)National Bio and Agro-Defense Facility (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020182020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/214533reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.vetimm.2018.11.018Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2145332026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
title Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
spellingShingle Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
Pérez-Núñez, Daniel
African swine fever virus
Heterologous DNA-protein immunization
Plasmid-expressed antigen
Recombinant protein
Immune response
Immunopathology
title_short Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
title_full Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
title_fullStr Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
title_full_unstemmed Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
title_sort Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs
dc.creator.none.fl_str_mv Pérez-Núñez, Daniel
Sunwoo, Sun-Young
Sánchez, Elena G.
Haley, Nicholas
García-Belmonte, Raquel
Nogal, Marisa
Morozov, Igor
Madden, Daniel
Gaudreault, Natasha N.
Mur, Lina
Shivana, Vinay
Richt, Jüergen A.
Revilla Novella, Yolanda
author Pérez-Núñez, Daniel
author_facet Pérez-Núñez, Daniel
Sunwoo, Sun-Young
Sánchez, Elena G.
Haley, Nicholas
García-Belmonte, Raquel
Nogal, Marisa
Morozov, Igor
Madden, Daniel
Gaudreault, Natasha N.
Mur, Lina
Shivana, Vinay
Richt, Jüergen A.
Revilla Novella, Yolanda
author_role author
author2 Sunwoo, Sun-Young
Sánchez, Elena G.
Haley, Nicholas
García-Belmonte, Raquel
Nogal, Marisa
Morozov, Igor
Madden, Daniel
Gaudreault, Natasha N.
Mur, Lina
Shivana, Vinay
Richt, Jüergen A.
Revilla Novella, Yolanda
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv US Department of Homeland Security
Center of Excellence for Emerging and Zoonotic Animal Diseases (US)
National Bio and Agro-Defense Facility (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv African swine fever virus
Heterologous DNA-protein immunization
Plasmid-expressed antigen
Recombinant protein
Immune response
Immunopathology
topic African swine fever virus
Heterologous DNA-protein immunization
Plasmid-expressed antigen
Recombinant protein
Immune response
Immunopathology
description African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/214533
url http://hdl.handle.net/10261/214533
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2018.11.018
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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