Protein nanoparticles are nontoxic, tuneable cell stressors

de Pinho Favaro, Marianna T.|||0000-0003-2942-247X; Sánchez-García, Laura|||0000-0002-8420-1701; Sánchez Chardi, Alejandro|||0000-0002-8789-1883; Roldán, Mònica; Unzueta Elorza, Ugutz|||0000-0001-5119-2266; Serna, Naroa|||0000-0001-5682-8198; Cano-Garrido, Olivia|||0000-0002-5504-2131; Azzoni, Adriano Rodrigues|||0000-0003-0696-4663; Ferrer-Miralles, Neus|||0000-0003-2981-3913; Villaverde, Antonio|||0000-0002-2615-4521; Vázquez, Esther|||0000-0003-1052-0424

Protein nanoparticles are nontoxic, tuneable cell stressors

Aim: nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. - Results: by repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generat...

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Autores:	de Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247X, Sánchez-García, Laura\|\|\|0000-0002-8420-1701, Sánchez Chardi, Alejandro\|\|\|0000-0002-8789-1883, Roldán, Mònica, Unzueta Elorza, Ugutz\|\|\|0000-0001-5119-2266, Serna, Naroa\|\|\|0000-0001-5682-8198, Cano-Garrido, Olivia\|\|\|0000-0002-5504-2131, Azzoni, Adriano Rodrigues\|\|\|0000-0003-0696-4663, Ferrer-Miralles, Neus\|\|\|0000-0003-2981-3913, Villaverde, Antonio\|\|\|0000-0002-2615-4521, Vázquez, Esther\|\|\|0000-0003-1052-0424
Tipo de recurso:	artículo
Fecha de publicación:	2018
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:236694
Acceso en línea:	https://ddd.uab.cat/record/236694 https://dx.doi.org/urn:doi:10.2217/nnm-2017-0294
Access Level:	acceso abierto
Palabra clave:	Molecular therapy Nanoparticles Recombinant proteins Self-assembling Stressor

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spelling	Protein nanoparticles are nontoxic, tuneable cell stressorsde Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247XSánchez-García, Laura\|\|\|0000-0002-8420-1701Sánchez Chardi, Alejandro\|\|\|0000-0002-8789-1883Roldán, MònicaUnzueta Elorza, Ugutz\|\|\|0000-0001-5119-2266Serna, Naroa\|\|\|0000-0001-5682-8198Cano-Garrido, Olivia\|\|\|0000-0002-5504-2131Azzoni, Adriano Rodrigues\|\|\|0000-0003-0696-4663Ferrer-Miralles, Neus\|\|\|0000-0003-2981-3913Villaverde, Antonio\|\|\|0000-0002-2615-4521Vázquez, Esther\|\|\|0000-0003-1052-0424Molecular therapyNanoparticlesRecombinant proteinsSelf-assemblingStressorAim: nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. - Results: by repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition. These materials, once exposed to cultured cells, are differentially internalized in absence of toxicity and trigger distinctive cell adaptive responses, monitored by the emission of tubular filopodia and enhanced drug sensitivity. - Conclusion: the capability to rapidly modulate such cell responses by conventional protein engineering reveals protein nanoparticles as tuneable, versatile and potent cell stressors for cell-targeted conditioning. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/236694https://dx.doi.org/urn:doi:10.2217/nnm-2017-0294reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-132Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/FIB100063Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00272open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2366942026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Protein nanoparticles are nontoxic, tuneable cell stressors
title	Protein nanoparticles are nontoxic, tuneable cell stressors
spellingShingle	Protein nanoparticles are nontoxic, tuneable cell stressors de Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247X Molecular therapy Nanoparticles Recombinant proteins Self-assembling Stressor
title_short	Protein nanoparticles are nontoxic, tuneable cell stressors
title_full	Protein nanoparticles are nontoxic, tuneable cell stressors
title_fullStr	Protein nanoparticles are nontoxic, tuneable cell stressors
title_full_unstemmed	Protein nanoparticles are nontoxic, tuneable cell stressors
title_sort	Protein nanoparticles are nontoxic, tuneable cell stressors
dc.creator.none.fl_str_mv	de Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247X Sánchez-García, Laura\|\|\|0000-0002-8420-1701 Sánchez Chardi, Alejandro\|\|\|0000-0002-8789-1883 Roldán, Mònica Unzueta Elorza, Ugutz\|\|\|0000-0001-5119-2266 Serna, Naroa\|\|\|0000-0001-5682-8198 Cano-Garrido, Olivia\|\|\|0000-0002-5504-2131 Azzoni, Adriano Rodrigues\|\|\|0000-0003-0696-4663 Ferrer-Miralles, Neus\|\|\|0000-0003-2981-3913 Villaverde, Antonio\|\|\|0000-0002-2615-4521 Vázquez, Esther\|\|\|0000-0003-1052-0424
author	de Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247X
author_facet	de Pinho Favaro, Marianna T.\|\|\|0000-0003-2942-247X Sánchez-García, Laura\|\|\|0000-0002-8420-1701 Sánchez Chardi, Alejandro\|\|\|0000-0002-8789-1883 Roldán, Mònica Unzueta Elorza, Ugutz\|\|\|0000-0001-5119-2266 Serna, Naroa\|\|\|0000-0001-5682-8198 Cano-Garrido, Olivia\|\|\|0000-0002-5504-2131 Azzoni, Adriano Rodrigues\|\|\|0000-0003-0696-4663 Ferrer-Miralles, Neus\|\|\|0000-0003-2981-3913 Villaverde, Antonio\|\|\|0000-0002-2615-4521 Vázquez, Esther\|\|\|0000-0003-1052-0424
author_role	author
author2	Sánchez-García, Laura\|\|\|0000-0002-8420-1701 Sánchez Chardi, Alejandro\|\|\|0000-0002-8789-1883 Roldán, Mònica Unzueta Elorza, Ugutz\|\|\|0000-0001-5119-2266 Serna, Naroa\|\|\|0000-0001-5682-8198 Cano-Garrido, Olivia\|\|\|0000-0002-5504-2131 Azzoni, Adriano Rodrigues\|\|\|0000-0003-0696-4663 Ferrer-Miralles, Neus\|\|\|0000-0003-2981-3913 Villaverde, Antonio\|\|\|0000-0002-2615-4521 Vázquez, Esther\|\|\|0000-0003-1052-0424
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Molecular therapy Nanoparticles Recombinant proteins Self-assembling Stressor
topic	Molecular therapy Nanoparticles Recombinant proteins Self-assembling Stressor
description	Aim: nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. - Results: by repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition. These materials, once exposed to cultured cells, are differentially internalized in absence of toxicity and trigger distinctive cell adaptive responses, monitored by the emission of tubular filopodia and enhanced drug sensitivity. - Conclusion: the capability to rapidly modulate such cell responses by conventional protein engineering reveals protein nanoparticles as tuneable, versatile and potent cell stressors for cell-targeted conditioning.
publishDate	2018
dc.date.none.fl_str_mv	2 2018-01-01 2018 2018-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/236694 https://dx.doi.org/urn:doi:10.2217/nnm-2017-0294
url	https://ddd.uab.cat/record/236694 https://dx.doi.org/urn:doi:10.2217/nnm-2017-0294
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-132 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/FIB100063 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PI15/00272
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Protein nanoparticles are nontoxic, tuneable cell stressors

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