Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide

Nitric oxide (NO) decreases cellular oxygen (O-2) consumption by competitively inhibiting cytochrome c oxidase. Here, we show that endogenously released endothelial NO, either basal or stimulated, can modulate O-2 consumption both throughout the thickness of conductance vessels and in the microcircu...

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Detalhes bibliográficos
Autores: Victor, VM, Nunez, C, D'Ocon, P, Taylor, CT, Esplugues, JV, Moncada, S
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10615
Acesso em linha:https://fisabio.portalinvestigacion.com/publicaciones/10615
Access Level:acceso abierto
Palavra-chave:nitric oxide
endothelium
oxygen consumption
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spelling Regulation of Oxygen Distribution in Tissues by Endothelial Nitric OxideVictor, VMNunez, CD'Ocon, PTaylor, CTEsplugues, JVMoncada, Snitric oxideendotheliumoxygen consumptionNitric oxide (NO) decreases cellular oxygen (O-2) consumption by competitively inhibiting cytochrome c oxidase. Here, we show that endogenously released endothelial NO, either basal or stimulated, can modulate O-2 consumption both throughout the thickness of conductance vessels and in the microcirculation. Furthermore, we have shown that such modulation regulates O-2 distribution to the surrounding tissues. We have demonstrated these effects by measuring O-2 consumption in blood vessels in a hypoxic chamber and O-2 distribution in the microcirculation using the fluorescent oxygen-probe Ru(phen)(3)(2+). Removal of NO by physical or pharmacological means, or in eNOS(-/-) mice, abolishes this regulatory mechanism. Our results indicate that, in addition to its well-known effect on the regulation of vascular tone, endothelial NO plays a major role in facilitating the distribution of O-2, an action which is crucial for the adaptation of tissues, including the vessel wall itself, to hypoxia. It is possible that changes in the distribution of O-2 throughout the vessel wall may be implicated in the origin of vascular pathologies such as atherosclerosis. (Circ Res. 2009;104:1178-1183.)LIPPINCOTT WILLIAMS & WILKINS2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10615CIRCULATION RESEARCHISSN: 00097330ISSNe: 15244571reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p106152026-06-11T12:45:17Z
dc.title.none.fl_str_mv Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
title Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
spellingShingle Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
Victor, VM
nitric oxide
endothelium
oxygen consumption
title_short Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
title_full Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
title_fullStr Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
title_full_unstemmed Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
title_sort Regulation of Oxygen Distribution in Tissues by Endothelial Nitric Oxide
dc.creator.none.fl_str_mv Victor, VM
Nunez, C
D'Ocon, P
Taylor, CT
Esplugues, JV
Moncada, S
author Victor, VM
author_facet Victor, VM
Nunez, C
D'Ocon, P
Taylor, CT
Esplugues, JV
Moncada, S
author_role author
author2 Nunez, C
D'Ocon, P
Taylor, CT
Esplugues, JV
Moncada, S
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv nitric oxide
endothelium
oxygen consumption
topic nitric oxide
endothelium
oxygen consumption
description Nitric oxide (NO) decreases cellular oxygen (O-2) consumption by competitively inhibiting cytochrome c oxidase. Here, we show that endogenously released endothelial NO, either basal or stimulated, can modulate O-2 consumption both throughout the thickness of conductance vessels and in the microcirculation. Furthermore, we have shown that such modulation regulates O-2 distribution to the surrounding tissues. We have demonstrated these effects by measuring O-2 consumption in blood vessels in a hypoxic chamber and O-2 distribution in the microcirculation using the fluorescent oxygen-probe Ru(phen)(3)(2+). Removal of NO by physical or pharmacological means, or in eNOS(-/-) mice, abolishes this regulatory mechanism. Our results indicate that, in addition to its well-known effect on the regulation of vascular tone, endothelial NO plays a major role in facilitating the distribution of O-2, an action which is crucial for the adaptation of tissues, including the vessel wall itself, to hypoxia. It is possible that changes in the distribution of O-2 throughout the vessel wall may be implicated in the origin of vascular pathologies such as atherosclerosis. (Circ Res. 2009;104:1178-1183.)
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10615
url https://fisabio.portalinvestigacion.com/publicaciones/10615
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv LIPPINCOTT WILLIAMS & WILKINS
publisher.none.fl_str_mv LIPPINCOTT WILLIAMS & WILKINS
dc.source.none.fl_str_mv CIRCULATION RESEARCH
ISSN: 00097330
ISSNe: 15244571
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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