Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification

Neural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of ce...

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Detalles Bibliográficos
Autores: Hoijman Kleinman, Esteban, Fargas, L., Blader, Patrick, Alsina, Berta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/185266
Acceso en línea:https://hdl.handle.net/2445/185266
Access Level:acceso abierto
Palabra clave:Biologia del desenvolupament
Morfogènesi
Cèl·lules mare
Neurociències
Developmental biology
Morphogenesis
Stem cells
Neurosciences
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spelling Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specificationHoijman Kleinman, EstebanFargas, L.Blader, PatrickAlsina, BertaBiologia del desenvolupamentMorfogènesiCèl·lules mareNeurociènciesDevelopmental biologyMorphogenesisStem cellsNeurosciencesNeural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of cell behaviours with dynamic quantification of proneural expression to uncover the construction of the zebrafish otic neurogenic domain. We identify pioneer cells expressing neurog1 outside the otic epithelium that migrate and ingress into the epithelialising placode to become the first otic neuronal progenitors. Subsequently, neighbouring cells express neurog1 inside the placode, and apical symmetric divisions amplify the specified pool. Interestingly, pioneer cells delaminate shortly after ingression. Ablation experiments reveal that pioneer cells promote neurog1 expression in other otic cells. Finally, ingression relies on the epithelialisation timing controlled by FGF activity. We propose a novel view for otic neurogenesis integrating cell dynamics whereby ingression of pioneer cells instructs neuronal specification.eLife Sciences2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/185266Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.7554/eLife.25543eLife, 2017https://doi.org/10.7554/eLife.25543cc-by (c) Hoijman Kleinman, Esteban et al., 2017https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1852662026-05-27T06:46:51Z
dc.title.none.fl_str_mv Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
title Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
spellingShingle Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
Hoijman Kleinman, Esteban
Biologia del desenvolupament
Morfogènesi
Cèl·lules mare
Neurociències
Developmental biology
Morphogenesis
Stem cells
Neurosciences
title_short Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
title_full Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
title_fullStr Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
title_full_unstemmed Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
title_sort Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification
dc.creator.none.fl_str_mv Hoijman Kleinman, Esteban
Fargas, L.
Blader, Patrick
Alsina, Berta
author Hoijman Kleinman, Esteban
author_facet Hoijman Kleinman, Esteban
Fargas, L.
Blader, Patrick
Alsina, Berta
author_role author
author2 Fargas, L.
Blader, Patrick
Alsina, Berta
author2_role author
author
author
dc.subject.none.fl_str_mv Biologia del desenvolupament
Morfogènesi
Cèl·lules mare
Neurociències
Developmental biology
Morphogenesis
Stem cells
Neurosciences
topic Biologia del desenvolupament
Morfogènesi
Cèl·lules mare
Neurociències
Developmental biology
Morphogenesis
Stem cells
Neurosciences
description Neural patterning involves regionalised cell specification. Recent studies indicate that cell dynamics play instrumental roles in neural pattern refinement and progression, but the impact of cell behaviour and morphogenesis on neural specification is not understood. Here we combine 4D analysis of cell behaviours with dynamic quantification of proneural expression to uncover the construction of the zebrafish otic neurogenic domain. We identify pioneer cells expressing neurog1 outside the otic epithelium that migrate and ingress into the epithelialising placode to become the first otic neuronal progenitors. Subsequently, neighbouring cells express neurog1 inside the placode, and apical symmetric divisions amplify the specified pool. Interestingly, pioneer cells delaminate shortly after ingression. Ablation experiments reveal that pioneer cells promote neurog1 expression in other otic cells. Finally, ingression relies on the epithelialisation timing controlled by FGF activity. We propose a novel view for otic neurogenesis integrating cell dynamics whereby ingression of pioneer cells instructs neuronal specification.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/185266
url https://hdl.handle.net/2445/185266
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.7554/eLife.25543
eLife, 2017
https://doi.org/10.7554/eLife.25543
dc.rights.none.fl_str_mv cc-by (c) Hoijman Kleinman, Esteban et al., 2017
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Hoijman Kleinman, Esteban et al., 2017
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences
publisher.none.fl_str_mv eLife Sciences
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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