Early anther ablation triggers parthenocarpic fruit development in tomato

[EN] Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that pro...

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Autores: Medina Herranz, Mónica, Roque Mesa, Edelin Marta, Rodríguez-Concepción, Manuel, Pineda Chaza, Benito José|||0000-0001-9176-701X, Cañas Clemente, Luís Antonio|||0000-0003-4177-4777, BELTRAN PORTER, JOSE PIO, Gómez Mena, María Concepción|||0000-0002-1272-3631
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/78884
Acceso en línea:https://riunet.upv.es/handle/10251/78884
Access Level:acceso abierto
Palabra clave:Fruit development
Tomato
Male sterility
Parthenocarpy
Nutrient value
GENETICA
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network_name_str España
repository_id_str
spelling Early anther ablation triggers parthenocarpic fruit development in tomatoMedina Herranz, MónicaRoque Mesa, Edelin MartaRodríguez-Concepción, ManuelPineda Chaza, Benito José|||0000-0001-9176-701XCañas Clemente, Luís Antonio|||0000-0003-4177-4777BELTRAN PORTER, JOSE PIOGómez Mena, María Concepción|||0000-0002-1272-3631Fruit developmentTomatoMale sterilityParthenocarpyNutrient valueGENETICA[EN] Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that produce parthenocarpic fruits in two tomato cultivars (Micro-Tom and Moneymaker). We generated male-sterility using the cytotoxic gene barnase targeted to the anthers with the PsEND1 anther-specific promoter. The ovaries of these plants grew in the absence of fertilization producing seedless, parthenocarpic fruits. Early anther ablation is essential to trigger the developing of the transgenic ovaries into fruits, in the absence of the signals usually generated during pollination and fertilization. Ovaries are fully functional and can be manually pollinated to obtain seeds. The transgenic plants obtained in the commercial cultivar Moneymaker show that the parthenocarpic development of the fruit does not have negative consequences in fruit quality. Throughout metabolomic analyses of the tomato fruits, we have identified two elite lines which showed increased levels of several health promoting metabolites and volatile compounds. Thus, early anther ablation can be considered a useful tool to promote fruit set and to obtain seedless and good quality fruits in tomato plants. These plants are also useful parental lines to be used in hybrid breeding approaches.This work was supported by grants from the Spanish Ministerio de Ciencia e Innovacion (MICINN, AGL2009-07617 to M.M.) and the Ramon y Cajal Program (RYC-2007-00627 to C.G.-M.). We thank Teresa Caballero, Aurora Medina, Rosa Rodriguez and Jose Luis Rambla for technical assistance with metabolomic analysis and Rafael Martinez for expert plant care.Wiley Open AccessInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Ciencia e InnovaciónMinisterio de Educación y CienciaRepositorio Institucional de la Universitat Politècnica de València Riunet20132013-08-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/78884reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2009-07617 Analisis Genetico De La Androesterilidad Como Herramienta Para La Mejora Del Cuajado Y La Calidad Del Fruto De Variedades Comerciales De TomateMinisterio de Educación y Ciencia https://doi.org/10.13039/501100008743 RYC-2007-00627 RYC-2007-00627open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/788842026-06-13T07:49:27Z
dc.title.none.fl_str_mv Early anther ablation triggers parthenocarpic fruit development in tomato
title Early anther ablation triggers parthenocarpic fruit development in tomato
spellingShingle Early anther ablation triggers parthenocarpic fruit development in tomato
Medina Herranz, Mónica
Fruit development
Tomato
Male sterility
Parthenocarpy
Nutrient value
GENETICA
title_short Early anther ablation triggers parthenocarpic fruit development in tomato
title_full Early anther ablation triggers parthenocarpic fruit development in tomato
title_fullStr Early anther ablation triggers parthenocarpic fruit development in tomato
title_full_unstemmed Early anther ablation triggers parthenocarpic fruit development in tomato
title_sort Early anther ablation triggers parthenocarpic fruit development in tomato
dc.creator.none.fl_str_mv Medina Herranz, Mónica
Roque Mesa, Edelin Marta
Rodríguez-Concepción, Manuel
Pineda Chaza, Benito José|||0000-0001-9176-701X
Cañas Clemente, Luís Antonio|||0000-0003-4177-4777
BELTRAN PORTER, JOSE PIO
Gómez Mena, María Concepción|||0000-0002-1272-3631
author Medina Herranz, Mónica
author_facet Medina Herranz, Mónica
Roque Mesa, Edelin Marta
Rodríguez-Concepción, Manuel
Pineda Chaza, Benito José|||0000-0001-9176-701X
Cañas Clemente, Luís Antonio|||0000-0003-4177-4777
BELTRAN PORTER, JOSE PIO
Gómez Mena, María Concepción|||0000-0002-1272-3631
author_role author
author2 Roque Mesa, Edelin Marta
Rodríguez-Concepción, Manuel
Pineda Chaza, Benito José|||0000-0001-9176-701X
Cañas Clemente, Luís Antonio|||0000-0003-4177-4777
BELTRAN PORTER, JOSE PIO
Gómez Mena, María Concepción|||0000-0002-1272-3631
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Ciencia e Innovación
Ministerio de Educación y Ciencia
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Fruit development
Tomato
Male sterility
Parthenocarpy
Nutrient value
GENETICA
topic Fruit development
Tomato
Male sterility
Parthenocarpy
Nutrient value
GENETICA
description [EN] Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male-sterile transgenic plants that produce parthenocarpic fruits in two tomato cultivars (Micro-Tom and Moneymaker). We generated male-sterility using the cytotoxic gene barnase targeted to the anthers with the PsEND1 anther-specific promoter. The ovaries of these plants grew in the absence of fertilization producing seedless, parthenocarpic fruits. Early anther ablation is essential to trigger the developing of the transgenic ovaries into fruits, in the absence of the signals usually generated during pollination and fertilization. Ovaries are fully functional and can be manually pollinated to obtain seeds. The transgenic plants obtained in the commercial cultivar Moneymaker show that the parthenocarpic development of the fruit does not have negative consequences in fruit quality. Throughout metabolomic analyses of the tomato fruits, we have identified two elite lines which showed increased levels of several health promoting metabolites and volatile compounds. Thus, early anther ablation can be considered a useful tool to promote fruit set and to obtain seedless and good quality fruits in tomato plants. These plants are also useful parental lines to be used in hybrid breeding approaches.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-08-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/78884
url https://riunet.upv.es/handle/10251/78884
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2009-07617 Analisis Genetico De La Androesterilidad Como Herramienta Para La Mejora Del Cuajado Y La Calidad Del Fruto De Variedades Comerciales De Tomate
Ministerio de Educación y Ciencia https://doi.org/10.13039/501100008743 RYC-2007-00627 RYC-2007-00627
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley Open Access
publisher.none.fl_str_mv Wiley Open Access
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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