Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction
Respiratory disorders caused by allergy have been associated to bronchiolar inflammation leading to life-threatening airway narrowing. However, whether airway allergy causes alveolar dysfunction contributing to the pathology of allergic asthma remains unaddressed. To explore whether airway allergy c...
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/362150 |
| Acceso en línea: | http://hdl.handle.net/10261/362150 https://api.elsevier.com/content/abstract/scopus_id/85159961552 |
| Access Level: | acceso abierto |
| Palabra clave: | airway allergy inflammation allergic asthma alveolar dysfunction alveolar macrophages (AM) monocytes pneumocyte hypertrophy surfactant dysfunction |
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Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| title |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| spellingShingle |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction Feo-Lucas, Lidia airway allergy inflammation allergic asthma alveolar dysfunction alveolar macrophages (AM) monocytes pneumocyte hypertrophy surfactant dysfunction |
| title_short |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| title_full |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| title_fullStr |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| title_full_unstemmed |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| title_sort |
Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunction |
| dc.creator.none.fl_str_mv |
Feo-Lucas, Lidia Godio, Cristina Minguito de la Escalera, María Álvarez Ladrón, Natalia Villarrubia, Laura H. Vega-Pérez, Adrián González-Cintado, Leticia Domínguez-Andrés, Jorge García-Fojeda, Belén Montero-Fernández, Carlos Casals, Cristina Autilio, Chiara Perez-Gil, J. Crainiciuc, Georgiana Hidalgo, Andrés López-Bravo, María |
| author |
Feo-Lucas, Lidia |
| author_facet |
Feo-Lucas, Lidia Godio, Cristina Minguito de la Escalera, María Álvarez Ladrón, Natalia Villarrubia, Laura H. Vega-Pérez, Adrián González-Cintado, Leticia Domínguez-Andrés, Jorge García-Fojeda, Belén Montero-Fernández, Carlos Casals, Cristina Autilio, Chiara Perez-Gil, J. Crainiciuc, Georgiana Hidalgo, Andrés López-Bravo, María |
| author_role |
author |
| author2 |
Godio, Cristina Minguito de la Escalera, María Álvarez Ladrón, Natalia Villarrubia, Laura H. Vega-Pérez, Adrián González-Cintado, Leticia Domínguez-Andrés, Jorge García-Fojeda, Belén Montero-Fernández, Carlos Casals, Cristina Autilio, Chiara Perez-Gil, J. Crainiciuc, Georgiana Hidalgo, Andrés López-Bravo, María |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Economía y Competitividad (España) Ministerio de Ciencia e Innovación (España) Comunidad de Madrid Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
airway allergy inflammation allergic asthma alveolar dysfunction alveolar macrophages (AM) monocytes pneumocyte hypertrophy surfactant dysfunction |
| topic |
airway allergy inflammation allergic asthma alveolar dysfunction alveolar macrophages (AM) monocytes pneumocyte hypertrophy surfactant dysfunction |
| description |
Respiratory disorders caused by allergy have been associated to bronchiolar inflammation leading to life-threatening airway narrowing. However, whether airway allergy causes alveolar dysfunction contributing to the pathology of allergic asthma remains unaddressed. To explore whether airway allergy causes alveolar dysfunction that might contribute to the pathology of allergic asthma, alveolar structural and functional alterations were analyzed during house dust mite (HDM)-induced airway allergy in mice, by flow cytometry, light and electron microscopy, monocyte transfer experiments, assessment of intra-alveolarly-located cells, analysis of alveolar macrophage regeneration in Cx3cr1 cre:R26-yfp chimeras, analysis of surfactant-associated proteins, and study of lung surfactant biophysical properties by captive bubble surfactometry. Our results demonstrate that HDM-induced airway allergic reactions caused severe alveolar dysfunction, leading to alveolar macrophage death, pneumocyte hypertrophy and surfactant dysfunction. SP-B/C proteins were reduced in allergic lung surfactant, that displayed a reduced efficiency to form surface-active films, increasing the risk of atelectasis. Original alveolar macrophages were replaced by monocyte-derived alveolar macrophages, that persisted at least two months after the resolution of allergy. Monocyte to alveolar macrophage transition occurred through an intermediate stage of pre-alveolar macrophage and was paralleled with translocation into the alveolar space, Siglec-F upregulation, and downregulation of CX3CR1. These data support that the severe respiratory disorders caused by asthmatic reactions not only result from bronchiolar inflammation, but additionally from alveolar dysfunction compromising an efficient gas exchange. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/362150 https://api.elsevier.com/content/abstract/scopus_id/85159961552 |
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http://hdl.handle.net/10261/362150 https://api.elsevier.com/content/abstract/scopus_id/85159961552 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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1869412220568338432 |
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Airway allergy causes alveolar macrophage death, profound alveolar disorganization and surfactant dysfunctionFeo-Lucas, LidiaGodio, CristinaMinguito de la Escalera, MaríaÁlvarez Ladrón, NataliaVillarrubia, Laura H.Vega-Pérez, AdriánGonzález-Cintado, LeticiaDomínguez-Andrés, JorgeGarcía-Fojeda, BelénMontero-Fernández, CarlosCasals, CristinaAutilio, ChiaraPerez-Gil, J.Crainiciuc, GeorgianaHidalgo, AndrésLópez-Bravo, Maríaairway allergy inflammationallergic asthmaalveolar dysfunctionalveolar macrophages (AM)monocytespneumocyte hypertrophysurfactant dysfunctionRespiratory disorders caused by allergy have been associated to bronchiolar inflammation leading to life-threatening airway narrowing. However, whether airway allergy causes alveolar dysfunction contributing to the pathology of allergic asthma remains unaddressed. To explore whether airway allergy causes alveolar dysfunction that might contribute to the pathology of allergic asthma, alveolar structural and functional alterations were analyzed during house dust mite (HDM)-induced airway allergy in mice, by flow cytometry, light and electron microscopy, monocyte transfer experiments, assessment of intra-alveolarly-located cells, analysis of alveolar macrophage regeneration in Cx3cr1 cre:R26-yfp chimeras, analysis of surfactant-associated proteins, and study of lung surfactant biophysical properties by captive bubble surfactometry. Our results demonstrate that HDM-induced airway allergic reactions caused severe alveolar dysfunction, leading to alveolar macrophage death, pneumocyte hypertrophy and surfactant dysfunction. SP-B/C proteins were reduced in allergic lung surfactant, that displayed a reduced efficiency to form surface-active films, increasing the risk of atelectasis. Original alveolar macrophages were replaced by monocyte-derived alveolar macrophages, that persisted at least two months after the resolution of allergy. Monocyte to alveolar macrophage transition occurred through an intermediate stage of pre-alveolar macrophage and was paralleled with translocation into the alveolar space, Siglec-F upregulation, and downregulation of CX3CR1. These data support that the severe respiratory disorders caused by asthmatic reactions not only result from bronchiolar inflammation, but additionally from alveolar dysfunction compromising an efficient gas exchange.This work was supported by the Ministerio de Economía y Competitividad (Grant SAF2015-69905 to CaA and Grant SAF2015-65307-R to CC), Ministerio de Ciencia e Innovación (Grants PGC2018-101899-B-100 and PID2021-122748OB-I00 to CaA, Grants RTI2018-094355‐B‐I00 and PID2021-123044OB-I00 to CC and Grant PID2021-124932OB-I00 to JP-G), and Comunidad de Madrid (Grant P2018/NMT4389 to JP-G).Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia e Innovación (España)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/362150https://api.elsevier.com/content/abstract/scopus_id/85159961552reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//SAF2015-65307-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-101899-B-I00info:eu-repo/grantAgreement/MINECO//SAF2015-69905-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122748OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094355-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-123044OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124932OB-I00Frontiers in immunologyapplication/pdfhttps://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1125984/fullSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3621502026-05-22T06:33:51Z |
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