Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity

Reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined pluripotency and self-renewal factors has taken stem cell technology to the forefront of regenerative medicine. However, a number of challenges remain in the field including efficient protocols and the threat of canc...

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Autores: Álvarez Palomo, Ana Belén, McLenachan, Samuel, Requena Osete, Jordi, Menchón Najas, Cristina, Barrot i Feixat, Carme, Chen, Fred K., Munné Bosch, Sergi, Edel, Michael John
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/66417
Acesso em linha:https://hdl.handle.net/2445/66417
Access Level:acceso abierto
Palavra-chave:Hormones vegetals
Cèl·lules mare embrionàries
Citoquines
Tumors
Ratolins (Animals de laboratori)
Plant hormones
Embryonic stem cells
Cytokines
Mice (Laboratory animals)
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spelling Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicityÁlvarez Palomo, Ana BelénMcLenachan, SamuelRequena Osete, JordiMenchón Najas, CristinaBarrot i Feixat, CarmeChen, Fred K.Munné Bosch, SergiEdel, Michael JohnHormones vegetalsCèl·lules mare embrionàriesCitoquinesTumorsRatolins (Animals de laboratori)Plant hormonesEmbryonic stem cellsCytokinesTumorsMice (Laboratory animals)Reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined pluripotency and self-renewal factors has taken stem cell technology to the forefront of regenerative medicine. However, a number of challenges remain in the field including efficient protocols and the threat of cancer. Reprogramming of plant somatic cells to plant embryonic stem cells using a combination of two plant hormones was discovered in 1957 and has been a routine university laboratory practical for over 30 years. The plant hormones responsible for cell reprogramming to pluripotency, indole-3-acetic acid (IAA) and isopentenyl adenosine (IPA), are present in human cells, leading to the exciting possibility that plant hormones might reprogram mammalian cells without genetic factors. We found that plant hormones on their own could not reprogram mammalian cells but increase the efficiency of the early formation of iPS cells combined with three defined genetic factors during the first 3 weeks of reprogramming by accelerating the cell cycle and regulating pluripotency genes. Moreover, the cytokinin IPA, a known human anticancer agent, reduced the threat of cancer of iPS cell in vitro by regulating key cancer and stem cell-related genes, most notably c-Myc and Igf-1. In conclusion, the plant hormones, auxin and cytokinin, are new small chemicals useful for enhancing early reprogramming efficiency of mammalian cells and reducing the threat of cancer from iPS cells. These findings suggest a novel role for plant hormones in the biology of mammalian cell plasticityMary Ann Liebert, Inc.2015201520142015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/66417Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1089/scd.2013.0292Stem Cells and Development, 2014, vol. 23, num. 6, p. 586-593http://dx.doi.org/10.1089/scd.2013.0292(c) Mary Ann Liebert, Inc., 2014info:eu-repo/semantics/openAccessoai:recercat.cat:2445/664172026-05-29T05:05:01Z
dc.title.none.fl_str_mv Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
title Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
spellingShingle Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
Álvarez Palomo, Ana Belén
Hormones vegetals
Cèl·lules mare embrionàries
Citoquines
Tumors
Ratolins (Animals de laboratori)
Plant hormones
Embryonic stem cells
Cytokines
Tumors
Mice (Laboratory animals)
title_short Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
title_full Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
title_fullStr Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
title_full_unstemmed Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
title_sort Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity
dc.creator.none.fl_str_mv Álvarez Palomo, Ana Belén
McLenachan, Samuel
Requena Osete, Jordi
Menchón Najas, Cristina
Barrot i Feixat, Carme
Chen, Fred K.
Munné Bosch, Sergi
Edel, Michael John
author Álvarez Palomo, Ana Belén
author_facet Álvarez Palomo, Ana Belén
McLenachan, Samuel
Requena Osete, Jordi
Menchón Najas, Cristina
Barrot i Feixat, Carme
Chen, Fred K.
Munné Bosch, Sergi
Edel, Michael John
author_role author
author2 McLenachan, Samuel
Requena Osete, Jordi
Menchón Najas, Cristina
Barrot i Feixat, Carme
Chen, Fred K.
Munné Bosch, Sergi
Edel, Michael John
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hormones vegetals
Cèl·lules mare embrionàries
Citoquines
Tumors
Ratolins (Animals de laboratori)
Plant hormones
Embryonic stem cells
Cytokines
Tumors
Mice (Laboratory animals)
topic Hormones vegetals
Cèl·lules mare embrionàries
Citoquines
Tumors
Ratolins (Animals de laboratori)
Plant hormones
Embryonic stem cells
Cytokines
Tumors
Mice (Laboratory animals)
description Reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined pluripotency and self-renewal factors has taken stem cell technology to the forefront of regenerative medicine. However, a number of challenges remain in the field including efficient protocols and the threat of cancer. Reprogramming of plant somatic cells to plant embryonic stem cells using a combination of two plant hormones was discovered in 1957 and has been a routine university laboratory practical for over 30 years. The plant hormones responsible for cell reprogramming to pluripotency, indole-3-acetic acid (IAA) and isopentenyl adenosine (IPA), are present in human cells, leading to the exciting possibility that plant hormones might reprogram mammalian cells without genetic factors. We found that plant hormones on their own could not reprogram mammalian cells but increase the efficiency of the early formation of iPS cells combined with three defined genetic factors during the first 3 weeks of reprogramming by accelerating the cell cycle and regulating pluripotency genes. Moreover, the cytokinin IPA, a known human anticancer agent, reduced the threat of cancer of iPS cell in vitro by regulating key cancer and stem cell-related genes, most notably c-Myc and Igf-1. In conclusion, the plant hormones, auxin and cytokinin, are new small chemicals useful for enhancing early reprogramming efficiency of mammalian cells and reducing the threat of cancer from iPS cells. These findings suggest a novel role for plant hormones in the biology of mammalian cell plasticity
publishDate 2014
dc.date.none.fl_str_mv 2014
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/66417
url https://hdl.handle.net/2445/66417
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1089/scd.2013.0292
Stem Cells and Development, 2014, vol. 23, num. 6, p. 586-593
http://dx.doi.org/10.1089/scd.2013.0292
dc.rights.none.fl_str_mv (c) Mary Ann Liebert, Inc., 2014
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Mary Ann Liebert, Inc., 2014
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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