Tubulin modifications in human gametes : from the oocytes spindle to the sperm flagellum : Characterization of tubulin post translational modifications in female meiosis and sperm pathologies
This thesis aimed to characterize the tubulin PTM profile of human oocytes and spermatozoa. Tubulin rich structures play critical roles in the cellular behavior of human gametes. Mutations in tubulin or related proteins can affect oocyte maturation and flagellum motility. We first focused on tubulin...
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| Format: | doctoral thesis |
| Status: | Published version |
| Publication Date: | 2021 |
| Country: | España |
| Institution: | CBUC, CESCA |
| Repository: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/670643 |
| Online Access: | http://hdl.handle.net/10803/670643 |
| Access Level: | Open access |
| Keyword: | Oocytes Tubulin Microtubules Post-translational modifications Spermatozoa Ovocitos Tubulina Microtúbulos Modificaciones postraduccionales Espermatozoide 575 |
| Summary: | This thesis aimed to characterize the tubulin PTM profile of human oocytes and spermatozoa. Tubulin rich structures play critical roles in the cellular behavior of human gametes. Mutations in tubulin or related proteins can affect oocyte maturation and flagellum motility. We first focused on tubulin post-translational modifications (PTMs) in the oocyte spindle and sperm flagellum. We characterized the PTM spindle profile of MII oocytes cultured in vitro and matured in vivo, and compared PTM enzyme transcript levels with two additional groups: GV and failed to mature oocytes. Further determination of the transcripts’ translational fate was performed using the cytoplasmic polyadenylation element code with verification experiments on Xenopus oocytes. Additionally, we sought to deteremine the pattern and levels of tubulin PTMs along the sperm tail and correlate these profiles with pathologies like asthenozoospermia and teratozoospermia. |
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