Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain

7 Pág.

Detalles Bibliográficos
Autores: Fayos, Manena, Sacristán, Carlos, Velarde, R., Ewbank, Ana Carolina, Sastre, Natalia, De La Torre, Ana I., Iglesias, Irene, González, Beatriz, Sacristán, Irene
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/368041
Acceso en línea:http://hdl.handle.net/10261/368041
https://api.elsevier.com/content/abstract/scopus_id/85202077333
Access Level:acceso abierto
Palabra clave:Candidatus Mycoplasma haematoparvum
Carnivores
Hemoplasmas
Iberian wolf
Mycoplasma haemocanis
id ES_83f5598bd9d2fa68a9c8d05de216be55
oai_identifier_str oai:digital.csic.es:10261/368041
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
title Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
spellingShingle Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
Fayos, Manena
Candidatus Mycoplasma haematoparvum
Carnivores
Hemoplasmas
Iberian wolf
Mycoplasma haemocanis
title_short Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
title_full Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
title_fullStr Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
title_full_unstemmed Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
title_sort Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, Spain
dc.creator.none.fl_str_mv Fayos, Manena
Sacristán, Carlos
Velarde, R.
Ewbank, Ana Carolina
Sastre, Natalia
De La Torre, Ana I.
Iglesias, Irene
González, Beatriz
Sacristán, Irene
author Fayos, Manena
author_facet Fayos, Manena
Sacristán, Carlos
Velarde, R.
Ewbank, Ana Carolina
Sastre, Natalia
De La Torre, Ana I.
Iglesias, Irene
González, Beatriz
Sacristán, Irene
author_role author
author2 Sacristán, Carlos
Velarde, R.
Ewbank, Ana Carolina
Sastre, Natalia
De La Torre, Ana I.
Iglesias, Irene
González, Beatriz
Sacristán, Irene
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gobierno de Cantabria
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Fayos, Manena [0000-0001-7093-7497]
Sacristán, Carlos [0000-0002-6111-6301]
Velarde, R. [0000-0003-3332-6405]
Ewbank, Ana Carolina [0000-0002-5617-9287]
Sastre, Natalia [0000-0002-0758-9124]
De La Torre, Ana I. [0000-0003-4560-5196]
Iglesias, Irene [0000-0003-3472-6873]
Sacristán, Irene [0000-0002-4169-4884]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Candidatus Mycoplasma haematoparvum
Carnivores
Hemoplasmas
Iberian wolf
Mycoplasma haemocanis
topic Candidatus Mycoplasma haematoparvum
Carnivores
Hemoplasmas
Iberian wolf
Mycoplasma haemocanis
description 7 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/368041
https://api.elsevier.com/content/abstract/scopus_id/85202077333
url http://hdl.handle.net/10261/368041
https://api.elsevier.com/content/abstract/scopus_id/85202077333
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI//JDC2022-048632-I
info:eu-repo/grantAgreement/AEI//FJC-2020-046311-I
info:eu-repo/grantAgreement/AEI//IJC2020-046019-I
Centro de Investigación en Sanidad Animal (CISA)
https://doi.org/10.1016/j.meegid.2024.105659
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Detection and characterization of hemotropic Mycoplasmas in Iberian wolves (Canis lupus signatus) of Cantabria, SpainFayos, ManenaSacristán, CarlosVelarde, R.Ewbank, Ana CarolinaSastre, NataliaDe La Torre, Ana I.Iglesias, IreneGonzález, BeatrizSacristán, IreneCandidatus Mycoplasma haematoparvumCarnivoresHemoplasmasIberian wolfMycoplasma haemocanis7 Pág.Hemoplasmas (hemotropic mycoplasmas) are uncultivable wall-less bacteria able to infect mammalian erythrocytes. Hemoplasmas can cause anemia, especially in immunocompromised hosts, predisposing to secondary infections and even leading to death. Between 2017 and 2023, spleen samples of 131 wild Iberian wolves (Canis lupus signatus) of Cantabria (Spain) were screened for Mycoplasma spp. using a real-time PCR able to amplify a 360 bp fragment of the 16S rRNA gene and confirmed by direct Sanger sequencing. Additional conventional PCRs were performed to screen for coinfections by different Mycoplasma species and to discriminate between Mycoplasma haemocanis/haemofelis (Mhc/Mhf). Overall, 24/131 (18.3%) animals were PCR-positive. Biological and environmental factors potentially promoting hemoplasma infection in this species were analyzed. Two different hemoplasma species were detected: Mhc/Mhf (18/131; 13.7%) and Candidatus Mycoplasma haematoparvum (CMhp) (3/131; 2.3%), each with one nucleotide sequence type (ntST); three other sequences were not classified. No Mhc/Mhf and CMhp coinfection were observed. The 12 Mhc/Mhf suitable for ribonuclease P RNA sequencing were confirmed as Mhc. Mhc ntST was 100% identical to a Mhc sequence previously obtained in domestic dogs (Canis lupus familiaris), and in wild Iberian wolves of northwestern Spain (Asturias and Galicia) at a similar prevalence to the one found herein, suggesting a high Mhc genetic homogeneity in this wild population. CMhp ntST was 100% identical to CMhp sequences from domestic dogs. To our knowledge, this is the first description of CMhp in the Iberian wolf. The high genetic similarity observed in Mhc and CMhp sequences, as well as their high similarity with domestic dog sequences, suggest its recent introduction, a high level of intraspecific transmission within the wild wolf population, and likely, interspecific transmission between wolves and domestic dogs.The study was funded by Dirección General de Montes y Biodiversidad - Consejería de Desarrollo Rural, Ganadería, Pesca y Alimentación de Cantabria. Ana Carolina Ewbank and Irene Sacristán are recipients of Juan de la Cierva Formación fellowships (JDC2022–048632-I and FJC2020–046311–I, respectively). Carlos Sacristán is recipient of a Juan de la Cierva incorporación fellowship (IJC2020–046019-I). The Juan de la Cierva formación and incorporación grants were funded by Agencia Estatal de Investigación/Ministerio de Ciencia, Innovación y Universidades.Peer reviewedElsevierGobierno de CantabriaAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Fayos, Manena [0000-0001-7093-7497]Sacristán, Carlos [0000-0002-6111-6301]Velarde, R. [0000-0003-3332-6405]Ewbank, Ana Carolina [0000-0002-5617-9287]Sastre, Natalia [0000-0002-0758-9124]De La Torre, Ana I. [0000-0003-4560-5196]Iglesias, Irene [0000-0003-3472-6873]Sacristán, Irene [0000-0002-4169-4884]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/368041https://api.elsevier.com/content/abstract/scopus_id/85202077333reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//JDC2022-048632-Iinfo:eu-repo/grantAgreement/AEI//FJC-2020-046311-Iinfo:eu-repo/grantAgreement/AEI//IJC2020-046019-ICentro de Investigación en Sanidad Animal (CISA)https://doi.org/10.1016/j.meegid.2024.105659Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3680412026-05-22T06:33:51Z
score 15,811543

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