Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach

[EN] Polycaprolactone (PCL) scaffolds have demonstrated an effectiveness in articular cartilage regeneration due to their biomechanical properties. On the other hand, alginate hydrogels generate a 3D environment with great chondrogenic potential. Our aim is to generate a mixed PCL/alginate scaffold...

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Autores: Milián, Lara, Oliver-Ferrándiz, Maria, Peregrín, Ignacio, Sancho-Tello, Maria, Martín-de-Llano, Jose Javier, Carda, Carmen, Mata, Manuel, Martínez-Ramos, Cristina|||0000-0002-6540-4714
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/221165
Acceso en línea:https://riunet.upv.es/handle/10251/221165
Access Level:acceso abierto
Palabra clave:PCL
Alginate
Cartilage regeneration
Chondrocyte
Tissue engineering
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spelling Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological ApproachMilián, LaraOliver-Ferrándiz, MariaPeregrín, IgnacioSancho-Tello, MariaMartín-de-Llano, Jose JavierCarda, CarmenMata, ManuelMartínez-Ramos, Cristina|||0000-0002-6540-4714PCLAlginateCartilage regenerationChondrocyteTissue engineering[EN] Polycaprolactone (PCL) scaffolds have demonstrated an effectiveness in articular cartilage regeneration due to their biomechanical properties. On the other hand, alginate hydrogels generate a 3D environment with great chondrogenic potential. Our aim is to generate a mixed PCL/alginate scaffold that combines the chondrogenic properties of the two biomaterials. Porous PCL scaffolds were manufactured using a modified salt-leaching method and embedded in a culture medium or alginate in the presence or absence of chondrocytes. The chondrogenic capacity was studied in vitro. Type II collagen and aggrecan were measured by immunofluorescence, cell morphology by F-actin fluorescence staining and gene expression of COL1A1, COL2A1, ACAN, COL10A1, VEGF, RUNX1 and SOX6 by reverse transcription polymerase chain reaction (RT-PCR). The biocompatibility of the scaffolds was determined in vivo using athymic nude mice and assessed by histopathological and morphometric analysis. Alginate improved the chondrogenic potential of PCL in vitro by increasing the expression of type II collagen and aggrecan, as well as other markers related to chondrogenesis. All scaffolds showed good biocompatibility in the in vivo model. The presence of cells in the scaffolds induced an increase in vascularization of the PCL/alginate scaffolds. The results presented here reinforce the benefits of the combined use of PCL and alginate for the regeneration of articular cartilage.This research was funded by the Ministry of Economy and Competitiveness of the Spanish Government, grant number PID2019-106099RB-C42 (M.M. and J.J.M.-d.-L.) and by the local government of the Comunitat Valenciana (Spain), grant number PROMETEO/2020/069 (C.C.). CIBER-BBNis funded by the Spanish VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and the Instituto de Salud Carlos III, with assistance from the European Regional Development Fund.MDPICentro de Biomateriales e Ingeniería TisularGeneralitat ValencianaInstituto de Salud Carlos IIIAgencia Estatal de InvestigaciónEuropean Regional Development FundCentro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y NanomedicinaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-04-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/221165reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C42 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DEL MICROENTORNO DEL TUMOR (TME) Y LA TRANSICION EPITELIO MESENQUIMA (EMT) EN CANCER DE PULMONGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F069open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2211652026-06-13T07:49:27Z
dc.title.none.fl_str_mv Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
title Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
spellingShingle Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
Milián, Lara
PCL
Alginate
Cartilage regeneration
Chondrocyte
Tissue engineering
title_short Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
title_full Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
title_fullStr Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
title_full_unstemmed Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
title_sort Alginate Improves the Chondrogenic Capacity of 3D PCL Scaffolds In Vitro: A Histological Approach
dc.creator.none.fl_str_mv Milián, Lara
Oliver-Ferrándiz, Maria
Peregrín, Ignacio
Sancho-Tello, Maria
Martín-de-Llano, Jose Javier
Carda, Carmen
Mata, Manuel
Martínez-Ramos, Cristina|||0000-0002-6540-4714
author Milián, Lara
author_facet Milián, Lara
Oliver-Ferrándiz, Maria
Peregrín, Ignacio
Sancho-Tello, Maria
Martín-de-Llano, Jose Javier
Carda, Carmen
Mata, Manuel
Martínez-Ramos, Cristina|||0000-0002-6540-4714
author_role author
author2 Oliver-Ferrándiz, Maria
Peregrín, Ignacio
Sancho-Tello, Maria
Martín-de-Llano, Jose Javier
Carda, Carmen
Mata, Manuel
Martínez-Ramos, Cristina|||0000-0002-6540-4714
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Biomateriales e Ingeniería Tisular
Generalitat Valenciana
Instituto de Salud Carlos III
Agencia Estatal de Investigación
European Regional Development Fund
Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv PCL
Alginate
Cartilage regeneration
Chondrocyte
Tissue engineering
topic PCL
Alginate
Cartilage regeneration
Chondrocyte
Tissue engineering
description [EN] Polycaprolactone (PCL) scaffolds have demonstrated an effectiveness in articular cartilage regeneration due to their biomechanical properties. On the other hand, alginate hydrogels generate a 3D environment with great chondrogenic potential. Our aim is to generate a mixed PCL/alginate scaffold that combines the chondrogenic properties of the two biomaterials. Porous PCL scaffolds were manufactured using a modified salt-leaching method and embedded in a culture medium or alginate in the presence or absence of chondrocytes. The chondrogenic capacity was studied in vitro. Type II collagen and aggrecan were measured by immunofluorescence, cell morphology by F-actin fluorescence staining and gene expression of COL1A1, COL2A1, ACAN, COL10A1, VEGF, RUNX1 and SOX6 by reverse transcription polymerase chain reaction (RT-PCR). The biocompatibility of the scaffolds was determined in vivo using athymic nude mice and assessed by histopathological and morphometric analysis. Alginate improved the chondrogenic potential of PCL in vitro by increasing the expression of type II collagen and aggrecan, as well as other markers related to chondrogenesis. All scaffolds showed good biocompatibility in the in vivo model. The presence of cells in the scaffolds induced an increase in vascularization of the PCL/alginate scaffolds. The results presented here reinforce the benefits of the combined use of PCL and alginate for the regeneration of articular cartilage.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/221165
url https://riunet.upv.es/handle/10251/221165
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C42 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DEL MICROENTORNO DEL TUMOR (TME) Y LA TRANSICION EPITELIO MESENQUIMA (EMT) EN CANCER DE PULMON
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2020%2F069
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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