Vertical organization of microbial communities in Salineta hypersaline wetland, Spain

Microbial communities inhabiting hypersaline wetlands, well adapted to the environmental fluctuations due to flooding and desiccation events, play a key role in the biogeochemical cycles, ensuring ecosystem service. To better understand the ecosystem functioning, we studied soil microbial communitie...

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Autores: Bourhane, Zeina, Cagnon, Christine, Castañeda, Carmen, Rodríguez Ochoa, Rafael, Álvaro-Fuentes, Jorge, Cravo-Laureau, Cristiana, Duran, Robert
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/464353
Acceso en línea:https://doi.org/10.3389/fmicb.2023.869907
https://hdl.handle.net/10459.1/464353
Access Level:acceso abierto
Palabra clave:Ephemeral hypersaline lakes
Hypersaline ecosystem
Functional redundancy
Reverse redox gradient
Archaeal biomarkers
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spelling Vertical organization of microbial communities in Salineta hypersaline wetland, SpainBourhane, ZeinaCagnon, ChristineCastañeda, CarmenRodríguez Ochoa, RafaelÁlvaro-Fuentes, JorgeCravo-Laureau, CristianaDuran, RobertEphemeral hypersaline lakesHypersaline ecosystemFunctional redundancyReverse redox gradientArchaeal biomarkersMicrobial communities inhabiting hypersaline wetlands, well adapted to the environmental fluctuations due to flooding and desiccation events, play a key role in the biogeochemical cycles, ensuring ecosystem service. To better understand the ecosystem functioning, we studied soil microbial communities of Salineta wetland (NE Spain) in dry and wet seasons in three different landscape stations representing situations characteristic of ephemeral saline lakes: S1 soil usually submerged, S2 soil intermittently flooded, and S3 soil with halophytes. Microbial community composition was determined according to different redox layers by 16S rRNA gene barcoding. We observed reversed redox gradient, negative at the surface and positive in depth, which was identified by PERMANOVA as the main factor explaining microbial distribution. The Pseudomonadota, Gemmatimonadota, Bacteroidota, Desulfobacterota, and Halobacteriota phyla were dominant in all stations. Linear discriminant analysis effect size (LEfSe) revealed that the upper soil surface layer was characterized by the predominance of operational taxonomic units (OTUs) affiliated to strictly or facultative anaerobic halophilic bacteria and archaea while the subsurface soil layer was dominated by an OTU affiliated to Roseibaca, an aerobic alkali-tolerant bacterium. In addition, the potential functional capabilities, inferred by PICRUSt2 analysis, involved in carbon, nitrogen, and sulfur cycles were similar in all samples, irrespective of the redox stratification, suggesting functional redundancy. Our findings show microbial community changes according to water flooding conditions, which represent useful information for biomonitoring and management of these wetlands whose extreme aridity and salinity conditions are exposed to irreversible changes due to human activities.We acknowledge the European program ERANET-MED through the AQUASALT (NMED-0003-01) project, the French Research Agency (ANR) through the ANR-17-NMED-0003-01, and the Spanish Research Agency (AEI) through the PCI2018-092999 project.Frontiers Media2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fmicb.2023.869907https://hdl.handle.net/10459.1/464353reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2018-092999Reproducció del document publicat a https://doi.org/10.3389/fmicb.2023.869907Frontiers in Microbiology, 2023, vol. 14cc-by (c) Zeina Bourhane et al., 2023Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4643532026-06-24T12:42:17Z
dc.title.none.fl_str_mv Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
title Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
spellingShingle Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
Bourhane, Zeina
Ephemeral hypersaline lakes
Hypersaline ecosystem
Functional redundancy
Reverse redox gradient
Archaeal biomarkers
title_short Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
title_full Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
title_fullStr Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
title_full_unstemmed Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
title_sort Vertical organization of microbial communities in Salineta hypersaline wetland, Spain
dc.creator.none.fl_str_mv Bourhane, Zeina
Cagnon, Christine
Castañeda, Carmen
Rodríguez Ochoa, Rafael
Álvaro-Fuentes, Jorge
Cravo-Laureau, Cristiana
Duran, Robert
author Bourhane, Zeina
author_facet Bourhane, Zeina
Cagnon, Christine
Castañeda, Carmen
Rodríguez Ochoa, Rafael
Álvaro-Fuentes, Jorge
Cravo-Laureau, Cristiana
Duran, Robert
author_role author
author2 Cagnon, Christine
Castañeda, Carmen
Rodríguez Ochoa, Rafael
Álvaro-Fuentes, Jorge
Cravo-Laureau, Cristiana
Duran, Robert
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ephemeral hypersaline lakes
Hypersaline ecosystem
Functional redundancy
Reverse redox gradient
Archaeal biomarkers
topic Ephemeral hypersaline lakes
Hypersaline ecosystem
Functional redundancy
Reverse redox gradient
Archaeal biomarkers
description Microbial communities inhabiting hypersaline wetlands, well adapted to the environmental fluctuations due to flooding and desiccation events, play a key role in the biogeochemical cycles, ensuring ecosystem service. To better understand the ecosystem functioning, we studied soil microbial communities of Salineta wetland (NE Spain) in dry and wet seasons in three different landscape stations representing situations characteristic of ephemeral saline lakes: S1 soil usually submerged, S2 soil intermittently flooded, and S3 soil with halophytes. Microbial community composition was determined according to different redox layers by 16S rRNA gene barcoding. We observed reversed redox gradient, negative at the surface and positive in depth, which was identified by PERMANOVA as the main factor explaining microbial distribution. The Pseudomonadota, Gemmatimonadota, Bacteroidota, Desulfobacterota, and Halobacteriota phyla were dominant in all stations. Linear discriminant analysis effect size (LEfSe) revealed that the upper soil surface layer was characterized by the predominance of operational taxonomic units (OTUs) affiliated to strictly or facultative anaerobic halophilic bacteria and archaea while the subsurface soil layer was dominated by an OTU affiliated to Roseibaca, an aerobic alkali-tolerant bacterium. In addition, the potential functional capabilities, inferred by PICRUSt2 analysis, involved in carbon, nitrogen, and sulfur cycles were similar in all samples, irrespective of the redox stratification, suggesting functional redundancy. Our findings show microbial community changes according to water flooding conditions, which represent useful information for biomonitoring and management of these wetlands whose extreme aridity and salinity conditions are exposed to irreversible changes due to human activities.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fmicb.2023.869907
https://hdl.handle.net/10459.1/464353
url https://doi.org/10.3389/fmicb.2023.869907
https://hdl.handle.net/10459.1/464353
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2018-092999
Reproducció del document publicat a https://doi.org/10.3389/fmicb.2023.869907
Frontiers in Microbiology, 2023, vol. 14
dc.rights.none.fl_str_mv cc-by (c) Zeina Bourhane et al., 2023
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Zeina Bourhane et al., 2023
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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