AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata)
The intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitr...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/101368 |
| Acceso en línea: | http://hdl.handle.net/10261/101368 |
| Access Level: | acceso abierto |
| Palabra clave: | Osmoregulation Arginine vasotocin Sea bream Salinity Water absorption |
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AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata)Martos-Sitcha, Juan AntonioGregório, Silvia FilipaCarvalho, Edison Samir M.Canario, Adelino V. M.Power, Deborah M.Mancera, Juan MiguelMartínez-Rodríguez, GonzaloFuentes, JuanOsmoregulationArginine vasotocinSea breamSalinityWater absorptionThe intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitro the putative role of AVT in intestinal ion transport in the sea bream (Sparus aurata). A cDNA encoding part of an AVT receptor was isolated and phylogenetic analysis revealed it clustered with the V1a2-type receptor clade. V1a2 transcripts were expressed throughout the gastrointestinal tract, from esophagus to rectum, and were most abundant in the rectum regardless of long-term exposure to external salinities of 12, 35 or 55p.p.t. Basolateral addition of AVT (10-6M) to the anterior intestine and rectum of sea bream adapted to 12, 35 or 55p.p.t. mounted in Ussing chambers produced rapid salinity and region dependent responses in short circuit current (Isc), always in the absorptive direction. In addition, AVT stimulation of absorptive Isc conformed to a dose-response curve, with significant effects achieved at 10-8M, which corresponds to physiological values of plasma AVT for this species. The effect of AVT on intestinal Isc was insensitive to the CFTR selective inhibitor NPPB (200μM) applied apically, but was completely abolished in the presence of apical bumetanide (200μM). We propose a role for AVT in the regulation of ion absorption in the intestine of the sea bream mediated by an absorptive bumetanide-sensitive mechanism, likely NKCC2. © 2013 Elsevier Inc.JAM-S is funded by Ministry of Education (Spain) through the program “Formación de Profesorado Universitario” (Ref: AP2008-01194). This work was partially supported by Ministry of Science and Education, Spain by Project AGL2010-14876 to JMM and by the Ministry of Science and Higher Education and European Social Funds through the Portuguese National Science Foundation by Project PTDC/MAR/104008/2008 to JF.Peer ReviewedElsevierMinisterio de Educación y Ciencia (España)European CommissionFundação para a Ciência e a Tecnologia (Portugal)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2014201420132014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/101368reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.ygcen.2013.07.017Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1013682026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| title |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| spellingShingle |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) Martos-Sitcha, Juan Antonio Osmoregulation Arginine vasotocin Sea bream Salinity Water absorption |
| title_short |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| title_full |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| title_fullStr |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| title_full_unstemmed |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| title_sort |
AVT is involved in the regulation of ion transport in the intestine of the sea bream (Sparus aurata) |
| dc.creator.none.fl_str_mv |
Martos-Sitcha, Juan Antonio Gregório, Silvia Filipa Carvalho, Edison Samir M. Canario, Adelino V. M. Power, Deborah M. Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo Fuentes, Juan |
| author |
Martos-Sitcha, Juan Antonio |
| author_facet |
Martos-Sitcha, Juan Antonio Gregório, Silvia Filipa Carvalho, Edison Samir M. Canario, Adelino V. M. Power, Deborah M. Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo Fuentes, Juan |
| author_role |
author |
| author2 |
Gregório, Silvia Filipa Carvalho, Edison Samir M. Canario, Adelino V. M. Power, Deborah M. Mancera, Juan Miguel Martínez-Rodríguez, Gonzalo Fuentes, Juan |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Educación y Ciencia (España) European Commission Fundação para a Ciência e a Tecnologia (Portugal) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Osmoregulation Arginine vasotocin Sea bream Salinity Water absorption |
| topic |
Osmoregulation Arginine vasotocin Sea bream Salinity Water absorption |
| description |
The intestine of marine fish plays a crucial role in ion homeostasis by selective processing of ingested fluid. Although arginine vasotocin (AVT) is suggested to play a role in ion regulation in fish, its action in the intestine has not been demonstrated. Thus, the present study investigated in vitro the putative role of AVT in intestinal ion transport in the sea bream (Sparus aurata). A cDNA encoding part of an AVT receptor was isolated and phylogenetic analysis revealed it clustered with the V1a2-type receptor clade. V1a2 transcripts were expressed throughout the gastrointestinal tract, from esophagus to rectum, and were most abundant in the rectum regardless of long-term exposure to external salinities of 12, 35 or 55p.p.t. Basolateral addition of AVT (10-6M) to the anterior intestine and rectum of sea bream adapted to 12, 35 or 55p.p.t. mounted in Ussing chambers produced rapid salinity and region dependent responses in short circuit current (Isc), always in the absorptive direction. In addition, AVT stimulation of absorptive Isc conformed to a dose-response curve, with significant effects achieved at 10-8M, which corresponds to physiological values of plasma AVT for this species. The effect of AVT on intestinal Isc was insensitive to the CFTR selective inhibitor NPPB (200μM) applied apically, but was completely abolished in the presence of apical bumetanide (200μM). We propose a role for AVT in the regulation of ion absorption in the intestine of the sea bream mediated by an absorptive bumetanide-sensitive mechanism, likely NKCC2. © 2013 Elsevier Inc. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013 2014 2014 2014 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
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article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/101368 |
| url |
http://hdl.handle.net/10261/101368 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1016/j.ygcen.2013.07.017 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869412159184699392 |
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15.81155 |