Microfluidics for engineering of lipid-based nanoparticles
Lipid-based nanoparticles are wide used in biomedical applications due to their biocompatibility and versatility. Therefore, using the right fabrication method is key to ensuring their quality and functionality. This thesis compares already stablished extrusion method with the novel microfluidic sys...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/452214 |
| Acceso en línea: | https://hdl.handle.net/2117/452214 |
| Access Level: | acceso abierto |
| Palabra clave: | Liposomes Nanoparticles Microfluidics Nanopartícules Microfluídica Àrees temàtiques de la UPC::Enginyeria dels materials::Assaig de materials |
| Sumario: | Lipid-based nanoparticles are wide used in biomedical applications due to their biocompatibility and versatility. Therefore, using the right fabrication method is key to ensuring their quality and functionality. This thesis compares already stablished extrusion method with the novel microfluidic system, Helix Biotech NOVA, for liposome preparation, evaluating size, polydispersity, zeta potential, and lipid yield across three different phosphatidylcholine formulations. Extrusion produced consistent liposomes averaging 130–140 nm with low polydispersity and was compatible with biomimetic vesicle fabrication using cell lysate, yielding stable vesicles with comparable protein loading. Microfluidics enabled rapid, single-step synthesis of smaller liposomes (<100 nm) with similar lipid yield. These results indicate that while extrusion is a reliable method with good reproducibility, microfluidics offers a scalable and efficient alternative for producing small liposomes. Overall, this work advances understanding of fabrication techniques for liposome fabrication. |
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