Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence

In this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE w...

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Detalles Bibliográficos
Autores: García-Cañas, Virginia, González García, Ramón, Cifuentes, Alejandro
Tipo de recurso: artículo
Fecha de publicación:2002
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/12110
Acceso en línea:http://hdl.handle.net/10261/12110
Access Level:acceso abierto
Palabra clave:DNA
Capillary gel electrophoresis
Polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
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spelling Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescenceGarcía-Cañas, VirginiaGonzález García, RamónCifuentes, AlejandroDNACapillary gel electrophoresisPolymerase chain reactiontransgenic maizeBt maizeGMOsUVLIFIn this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.Peer reviewedAmerican Chemical Society200920092002info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_650188 bytestext/plainhttp://hdl.handle.net/10261/12110reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://pubs.acs.org/doi/abs/10.1021/jf011033ginfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/121102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
title Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
spellingShingle Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
García-Cañas, Virginia
DNA
Capillary gel electrophoresis
Polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
title_short Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
title_full Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
title_fullStr Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
title_full_unstemmed Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
title_sort Detection of genetically modified maize by PCR and capillary gel electrophoresis with UV detection and laser induced fluorescence
dc.creator.none.fl_str_mv García-Cañas, Virginia
González García, Ramón
Cifuentes, Alejandro
author García-Cañas, Virginia
author_facet García-Cañas, Virginia
González García, Ramón
Cifuentes, Alejandro
author_role author
author2 González García, Ramón
Cifuentes, Alejandro
author2_role author
author
dc.subject.none.fl_str_mv DNA
Capillary gel electrophoresis
Polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
topic DNA
Capillary gel electrophoresis
Polymerase chain reaction
transgenic maize
Bt maize
GMOs
UV
LIF
description In this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.
publishDate 2002
dc.date.none.fl_str_mv 2002
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/12110
url http://hdl.handle.net/10261/12110
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://pubs.acs.org/doi/abs/10.1021/jf011033g
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 88 bytes
text/plain
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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