The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes

Malaria mosquitoes acoustically detect mating partners through their flight tones in noisy swarms. Here, we study the auditory role of GABA, one of the main efferent signaling molecules in the mosquito ear, and its interactions with octopamine. We show that GABA signals through the GABAA receptor re...

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Autores: Ellis, David, Alampounti, Alexandros C., Suppermpool, Anya, Georgiades, Marcos, Freeman, Elizabeth Anna, Bagi, Judit, Tytheridge, Scott, Terrazas Duque, Daniela, Albert, Joerg T., Andrés, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/403625
Acceso en línea:http://hdl.handle.net/10261/403625
https://api.elsevier.com/content/abstract/scopus_id/105013480263
Access Level:acceso abierto
Palabra clave:Entomology
Molecular biology
Neuroscience
Pharmacology
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spelling The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoesEllis, DavidAlampounti, Alexandros C.Suppermpool, AnyaGeorgiades, MarcosFreeman, Elizabeth AnnaBagi, JuditTytheridge, ScottTerrazas Duque, DanielaAlbert, Joerg T.Andrés, MartaEntomologyMolecular biologyNeurosciencePharmacologyMalaria mosquitoes acoustically detect mating partners through their flight tones in noisy swarms. Here, we study the auditory role of GABA, one of the main efferent signaling molecules in the mosquito ear, and its interactions with octopamine. We show that GABA signals through the GABAA receptor resistance to Dieldrin (RDL), an insecticide target. Using picrotoxin to antagonize RDL receptors, we report multilevel auditory effects of GABA. Mechanically, picrotoxin promotes changes in the ear's anatomy and mechanical state. Because these effects are abolished in octopamine receptor AgOctβ2 knockouts, they appear to be octopamine dependent. Electrically, picrotoxin increases the spontaneous firing of the antennal nerve and enhances mechanically evoked responses. These effects are partially present in AgOctβ2 mutants, suggesting that RDL modulates electrical responses via octopamine-independent pathways. Together, our experiments show an important role of RDL in controlling malaria mosquito auditory sensitivity and implicate RDL in mating of natural malaria mosquito populations.This work received funding from UK Research and Innovation under the Future Leaders Fellowship scheme (grant reference MR/S015493/1 and MR/Y011732/1 to M.A.), a UCL Global Challenge Research Fund, a UCL Engagement Fund, a Wellcome ISSF grant (204841/Z/16/Z), a State Research Agency ATRAE Programme grant (ATR2023-145654), and Plan Generación de Conocimiento 2024 (PID2023-146360OA-I00) to M.A. This work was supported by a grant from the Biotechnology and Biological Sciences Research Council, UK (BBSRC, BB/V007866/1 to J.T.A.), a grant from The Human Frontier Science Program (HFSP grant RGP0033/2021 to J.T.A.), and from the European Union’s Horizon 2020 research and innovation programme (H2020-ERC-2014- CoG/648709/Clock Mechanics, to J.T.A.) D.A.E. is supported by a Marie Skłodowska Curie Actions Global Postdoctoral Fellowship, underwritten by the UK Research and Innovation (EP/Z001994/1: HORIZON-MSCA-2023-PF-01-01). M.A. is part of the CSIC’s GlobalHealth Plaform (PTI Salud Global).Peer reviewedElsevierUK Research and InnovationUniversity College LondonAgencia Estatal de Investigación (España)Biotechnology and Biological Sciences Research Council (UK)Human Frontier Science ProgramEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/403625https://api.elsevier.com/content/abstract/scopus_id/105013480263reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146360OA-I00info:eu-repo/grantAgreement/EC/H2020/648709The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2025.113264https://doi.org/10.1016/j.isci.2025.113264Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4036252026-05-22T06:33:51Z
dc.title.none.fl_str_mv The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
title The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
spellingShingle The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
Ellis, David
Entomology
Molecular biology
Neuroscience
Pharmacology
title_short The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
title_full The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
title_fullStr The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
title_full_unstemmed The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
title_sort The GABAA receptor RDL modulates the auditory sensitivity of malaria mosquitoes
dc.creator.none.fl_str_mv Ellis, David
Alampounti, Alexandros C.
Suppermpool, Anya
Georgiades, Marcos
Freeman, Elizabeth Anna
Bagi, Judit
Tytheridge, Scott
Terrazas Duque, Daniela
Albert, Joerg T.
Andrés, Marta
author Ellis, David
author_facet Ellis, David
Alampounti, Alexandros C.
Suppermpool, Anya
Georgiades, Marcos
Freeman, Elizabeth Anna
Bagi, Judit
Tytheridge, Scott
Terrazas Duque, Daniela
Albert, Joerg T.
Andrés, Marta
author_role author
author2 Alampounti, Alexandros C.
Suppermpool, Anya
Georgiades, Marcos
Freeman, Elizabeth Anna
Bagi, Judit
Tytheridge, Scott
Terrazas Duque, Daniela
Albert, Joerg T.
Andrés, Marta
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UK Research and Innovation
University College London
Agencia Estatal de Investigación (España)
Biotechnology and Biological Sciences Research Council (UK)
Human Frontier Science Program
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Entomology
Molecular biology
Neuroscience
Pharmacology
topic Entomology
Molecular biology
Neuroscience
Pharmacology
description Malaria mosquitoes acoustically detect mating partners through their flight tones in noisy swarms. Here, we study the auditory role of GABA, one of the main efferent signaling molecules in the mosquito ear, and its interactions with octopamine. We show that GABA signals through the GABAA receptor resistance to Dieldrin (RDL), an insecticide target. Using picrotoxin to antagonize RDL receptors, we report multilevel auditory effects of GABA. Mechanically, picrotoxin promotes changes in the ear's anatomy and mechanical state. Because these effects are abolished in octopamine receptor AgOctβ2 knockouts, they appear to be octopamine dependent. Electrically, picrotoxin increases the spontaneous firing of the antennal nerve and enhances mechanically evoked responses. These effects are partially present in AgOctβ2 mutants, suggesting that RDL modulates electrical responses via octopamine-independent pathways. Together, our experiments show an important role of RDL in controlling malaria mosquito auditory sensitivity and implicate RDL in mating of natural malaria mosquito populations.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/403625
https://api.elsevier.com/content/abstract/scopus_id/105013480263
url http://hdl.handle.net/10261/403625
https://api.elsevier.com/content/abstract/scopus_id/105013480263
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146360OA-I00
info:eu-repo/grantAgreement/EC/H2020/648709
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2025.113264
https://doi.org/10.1016/j.isci.2025.113264
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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