Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools

Mumps is a vaccine-preventable disease caused by the mumps virus (MuV). However, MuV has re-emerged in many countries with high vaccine coverage. The World Health Organization (WHO) recommends molecular surveillance based on sequencing of the small hydrophobic (SH) gene. Additionally, the combined u...

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Detalles Bibliográficos
Autores: Gavilán, Ana M, Perán-Ramos, Paula, Sanz, Juan Carlos, García-Comas, Luis, Pérez-Abeledo, Marta, Castellanos-Nadal, Ana Maria, Berciano, Jose Miguel, Lopez-Perea, Noemi, Masa-Calles, Josefa, Echevarria, Juan Emilio, Fernandez-Garcia, Aurora
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17179
Acceso en línea:http://hdl.handle.net/20.500.12105/17179
Access Level:acceso abierto
Palabra clave:Mumps virus
Mumps
Humans
Phylogeny
Disease Outbreaks
Genotype
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spelling Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular ToolsGavilán, Ana MPerán-Ramos, PaulaSanz, Juan CarlosGarcía-Comas, LuisPérez-Abeledo, MartaCastellanos-Nadal, Ana MariaBerciano, Jose MiguelLopez-Perea, NoemiMasa-Calles, JosefaEchevarria, Juan EmilioFernandez-Garcia, AuroraMumps virusMumpsHumansPhylogenyDisease OutbreaksGenotypeMumps is a vaccine-preventable disease caused by the mumps virus (MuV). However, MuV has re-emerged in many countries with high vaccine coverage. The World Health Organization (WHO) recommends molecular surveillance based on sequencing of the small hydrophobic (SH) gene. Additionally, the combined use of SH and non-coding regions (NCR) has been described in different studies, proving to be a useful complement marker to discriminate general patterns of circulation at national and international levels. The aim of this work is to test local-level usefulness of the combination of SH and MF-NCR sequencing in tracing hidden transmission clusters and chains during the last epidemic wave (2015-2020) in Spain. A database with 903 cases from the Autonomous Community of Madrid was generated by the integration of microbiological and epidemiological data. Of these, 453 representative cases were genotyped. Eight different SH variants and thirty-four SH haplotypes were detected. Local MuV circulation showed the same temporal pattern previously described at a national level. Only two of the thirteen previously identified outbreaks were caused by more than one variant/haplotype. Geographical representation of SH variants allowed the identification of several previously undetected clusters, which were analysed phylogenetically by the combination of SH and MF-NCR, in a total of 90 cases. MF-NCR was not able to improve the discrimination of geographical clusters based on SH sequencing, showing limited resolution for outbreak investigations.Multidisciplinary Digital Publishing Institute (MDPI)Centro de Investigación Biomédica en Red - CIBERESP (Epidemiología y Salud Pública)Instituto de Salud Carlos III20242024-01-1620232023-12-1320232023-12-13research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/20.500.12105/17179reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/171792026-06-12T12:43:37Z
dc.title.none.fl_str_mv Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
title Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
spellingShingle Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
Gavilán, Ana M
Mumps virus
Mumps
Humans
Phylogeny
Disease Outbreaks
Genotype
title_short Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
title_full Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
title_fullStr Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
title_full_unstemmed Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
title_sort Investigating Local Patterns of Mumps Virus Circulation, Using a Combination of Molecular Tools
dc.creator.none.fl_str_mv Gavilán, Ana M
Perán-Ramos, Paula
Sanz, Juan Carlos
García-Comas, Luis
Pérez-Abeledo, Marta
Castellanos-Nadal, Ana Maria
Berciano, Jose Miguel
Lopez-Perea, Noemi
Masa-Calles, Josefa
Echevarria, Juan Emilio
Fernandez-Garcia, Aurora
author Gavilán, Ana M
author_facet Gavilán, Ana M
Perán-Ramos, Paula
Sanz, Juan Carlos
García-Comas, Luis
Pérez-Abeledo, Marta
Castellanos-Nadal, Ana Maria
Berciano, Jose Miguel
Lopez-Perea, Noemi
Masa-Calles, Josefa
Echevarria, Juan Emilio
Fernandez-Garcia, Aurora
author_role author
author2 Perán-Ramos, Paula
Sanz, Juan Carlos
García-Comas, Luis
Pérez-Abeledo, Marta
Castellanos-Nadal, Ana Maria
Berciano, Jose Miguel
Lopez-Perea, Noemi
Masa-Calles, Josefa
Echevarria, Juan Emilio
Fernandez-Garcia, Aurora
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Investigación Biomédica en Red - CIBERESP (Epidemiología y Salud Pública)
Instituto de Salud Carlos III
dc.subject.none.fl_str_mv Mumps virus
Mumps
Humans
Phylogeny
Disease Outbreaks
Genotype
topic Mumps virus
Mumps
Humans
Phylogeny
Disease Outbreaks
Genotype
description Mumps is a vaccine-preventable disease caused by the mumps virus (MuV). However, MuV has re-emerged in many countries with high vaccine coverage. The World Health Organization (WHO) recommends molecular surveillance based on sequencing of the small hydrophobic (SH) gene. Additionally, the combined use of SH and non-coding regions (NCR) has been described in different studies, proving to be a useful complement marker to discriminate general patterns of circulation at national and international levels. The aim of this work is to test local-level usefulness of the combination of SH and MF-NCR sequencing in tracing hidden transmission clusters and chains during the last epidemic wave (2015-2020) in Spain. A database with 903 cases from the Autonomous Community of Madrid was generated by the integration of microbiological and epidemiological data. Of these, 453 representative cases were genotyped. Eight different SH variants and thirty-four SH haplotypes were detected. Local MuV circulation showed the same temporal pattern previously described at a national level. Only two of the thirteen previously identified outbreaks were caused by more than one variant/haplotype. Geographical representation of SH variants allowed the identification of several previously undetected clusters, which were analysed phylogenetically by the combination of SH and MF-NCR, in a total of 90 cases. MF-NCR was not able to improve the discrimination of geographical clusters based on SH sequencing, showing limited resolution for outbreak investigations.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-12-13
2023
2023-12-13
2024
2024-01-16
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/17179
url http://hdl.handle.net/20.500.12105/17179
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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