HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity

(This article belongs to the Special Issue Molecular and Neuromuscular Mechanisms in Skeletal Muscle Aging)

Detalles Bibliográficos
Autores: Pérez-Castro, Miguel Ángel, Hernández-Rasco, Francisco, Alonso-Bellido, Isabel María, Letrán-Sánchez, María S, Pérez-Villegas, Eva María, Vitallé, Joana, Real, Luis Miguel, Ruiz-Mateos, Ezequiel, Venero, José Luis, Tabares, Lucía, Carrión, Ángel Manuel, Armengol, José Ángel, Bachiller, Sara, Ruiz, Rocío
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/391188
Acceso en línea:http://hdl.handle.net/10261/391188
https://api.elsevier.com/content/abstract/scopus_id/85216088496
Access Level:acceso abierto
Palabra clave:Autophagy
Neuromuscular junction
Proteasome
Synapses
Vesicles
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oai_identifier_str oai:digital.csic.es:10261/391188
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
title HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
spellingShingle HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
Pérez-Castro, Miguel Ángel
Autophagy
Neuromuscular junction
Proteasome
Synapses
Vesicles
title_short HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
title_full HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
title_fullStr HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
title_full_unstemmed HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
title_sort HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal Activity
dc.creator.none.fl_str_mv Pérez-Castro, Miguel Ángel
Hernández-Rasco, Francisco
Alonso-Bellido, Isabel María
Letrán-Sánchez, María S
Pérez-Villegas, Eva María
Vitallé, Joana
Real, Luis Miguel
Ruiz-Mateos, Ezequiel
Venero, José Luis
Tabares, Lucía
Carrión, Ángel Manuel
Armengol, José Ángel
Bachiller, Sara
Ruiz, Rocío
author Pérez-Castro, Miguel Ángel
author_facet Pérez-Castro, Miguel Ángel
Hernández-Rasco, Francisco
Alonso-Bellido, Isabel María
Letrán-Sánchez, María S
Pérez-Villegas, Eva María
Vitallé, Joana
Real, Luis Miguel
Ruiz-Mateos, Ezequiel
Venero, José Luis
Tabares, Lucía
Carrión, Ángel Manuel
Armengol, José Ángel
Bachiller, Sara
Ruiz, Rocío
author_role author
author2 Hernández-Rasco, Francisco
Alonso-Bellido, Isabel María
Letrán-Sánchez, María S
Pérez-Villegas, Eva María
Vitallé, Joana
Real, Luis Miguel
Ruiz-Mateos, Ezequiel
Venero, José Luis
Tabares, Lucía
Carrión, Ángel Manuel
Armengol, José Ángel
Bachiller, Sara
Ruiz, Rocío
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
European Commission
Pérez-Castro, Miguel Ángel [0000-0003-2850-3470]
Alonso-Bellido, Isabel María [0000-0002-7475-8653]
Vitallé, Joana [0000-0002-5292-1851]
Ruiz-Mateos, Ezequiel [0000-0001-6747-7813]
Venero, José Luis [0000-0003-1137-8706]
Tabares, Lucía [0000-0002-4027-4044]
Armengol, José Ángel [0000-0002-9452-7828]
Bachiller, Sara [0000-0002-9000-3787]
Ruiz, Rocío [0000-0001-5142-9972]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Autophagy
Neuromuscular junction
Proteasome
Synapses
Vesicles
topic Autophagy
Neuromuscular junction
Proteasome
Synapses
Vesicles
description (This article belongs to the Special Issue Molecular and Neuromuscular Mechanisms in Skeletal Muscle Aging)
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/391188
https://api.elsevier.com/content/abstract/scopus_id/85216088496
url http://hdl.handle.net/10261/391188
https://api.elsevier.com/content/abstract/scopus_id/85216088496
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124096OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109569GB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ijms26020793
https://dx.doi.org/10.3390/ijms26020793
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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spelling HERC1 E3 Ubiquitin Ligase Is Necessary for Autophagy Processes and for the Maintenance and Homeostasis of Vesicles in Motor Nerve Terminals, but Not for Proteasomal ActivityPérez-Castro, Miguel ÁngelHernández-Rasco, FranciscoAlonso-Bellido, Isabel MaríaLetrán-Sánchez, María SPérez-Villegas, Eva MaríaVitallé, JoanaReal, Luis MiguelRuiz-Mateos, EzequielVenero, José LuisTabares, LucíaCarrión, Ángel ManuelArmengol, José ÁngelBachiller, SaraRuiz, RocíoAutophagyNeuromuscular junctionProteasomeSynapsesVesicles(This article belongs to the Special Issue Molecular and Neuromuscular Mechanisms in Skeletal Muscle Aging)The ubiquitin proteasome system (UPS) is implicated in protein homeostasis. One of the proteins involved in this system is HERC1 E3 ubiquitin ligase, which was associated with several processes including the normal development and neurotransmission at the neuromuscular junction (NMJ), autophagy in projection neurons, myelination of the peripheral nervous system, among others. The tambaleante (tbl) mouse model carries the spontaneous mutation Gly483Glu substitution in the HERC1 E3 protein. Using this model, we analyzed the implication of HERC1 E3 ubiquitin ligase in the activity of UPS, autophagy, and synaptic homeostasis in brain and muscle tissues. Regarding UPS, no differences were found in its activity nor in the specific gene expression in both brain and muscle tissues from tbl compared with the control littermates. Furthermore, the use of the specific UPS inhibitor (MG-132), did not alter the evoked neurotransmitter release in the levator auris longus (LAL) muscle. Interestingly, the expression of the autophagy-related gene p62 was significantly increased in the muscle of tbl compared to the control littermates. Indeed, impaired evoked neurotransmitter release was observed with the autophagy inhibitor Wortmannin. Finally, altered levels of Clathrin and Synaptophysin were detected in muscle tissues. Altogether, our findings show that HERC1 E3 ubiquitin ligase mutation found in tbl mice alters autophagy and vesicular recycling without affecting proteasomal function.This research was funded by the Ministerio de Ciencia e Innovación (PID2021-124096OB-I00) and Junta de Andalucía, Consejería de Economía y Conocimiento (P18-RT-1372). S.B. was supported by Ramón y Cajal 2021 research grant (RYC-2021-031661-I, funded by MCIN/AEI/10.13039/501100011033 and by European Union NextGenerationEU/PRTR). J.Á.A. and E.M.P.-V. were funded by the MINECO grant PID2019-109569GB-I00.Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Junta de AndalucíaEuropean CommissionPérez-Castro, Miguel Ángel [0000-0003-2850-3470]Alonso-Bellido, Isabel María [0000-0002-7475-8653]Vitallé, Joana [0000-0002-5292-1851]Ruiz-Mateos, Ezequiel [0000-0001-6747-7813]Venero, José Luis [0000-0003-1137-8706]Tabares, Lucía [0000-0002-4027-4044]Armengol, José Ángel [0000-0002-9452-7828]Bachiller, Sara [0000-0002-9000-3787]Ruiz, Rocío [0000-0001-5142-9972]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/391188https://api.elsevier.com/content/abstract/scopus_id/85216088496reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124096OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109569GB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ijms26020793https://dx.doi.org/10.3390/ijms26020793Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3911882026-05-22T06:33:51Z
score 15,812429

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