Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse

With the aim of studying delta-like protein 1 (DLK1) with respect to the relationship between adipocyte leptin and adenohypophyseal hormones, we carried out an immunohistochemical study analysing the presence of receptors for these hormones in the pituitary and adipose cells of male wild-type (WT) m...

Descripción completa

Detalles Bibliográficos
Autores: Rodríguez Bello, Aixa, Puertas-Avendaño, Ricardo A., González-Gómez, María Julia, González-Gómez, Miriam, Laborda, Jorge, Damas, Carmen, Ruiz-Hidalgo, María José, Díaz Delgado, Carmen
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/15491
Acceso en línea:http://hdl.handle.net/10578/15491
Access Level:acceso abierto
Palabra clave:Anterior pituitary
Hormone receptors
Leptin receptor
Leptin
id ES_82e2f9fe35fa1b9728eb08f54a55afa2
oai_identifier_str oai:ruidera.uclm.es:10578/15491
network_acronym_str ES
network_name_str España
repository_id_str
spelling Delta-like protein 1 in the pituitary-adipose axis in the adult male mouseRodríguez Bello, AixaPuertas-Avendaño, Ricardo A.González-Gómez, María JuliaGonzález-Gómez, MiriamLaborda, JorgeDamas, CarmenRuiz-Hidalgo, María JoséDíaz Delgado, CarmenAnterior pituitaryHormone receptorsLeptin receptorLeptinWith the aim of studying delta-like protein 1 (DLK1) with respect to the relationship between adipocyte leptin and adenohypophyseal hormones, we carried out an immunohistochemical study analysing the presence of receptors for these hormones in the pituitary and adipose cells of male wild-type (WT) mice (Dlk1+/+) compared to knockout (KO) mice (Dlk1−/−). The mRNA expression of these molecules was also determined using the reverse transcriptase-polymerase chain reaction. The results obtained showed that, in WT adipose cells, all of the adenohypophyseal hormone receptors were present, with a higher mRNA expression for growth hormone (GH) receptor and thyroid-stimulating hormone (TSH) receptor. Of the total cells in the anterior pituitary lobe, 17.09±0.9% were leptin receptor (LEPR) immunoreactive (-IR), mainly in GH-IR and prolactin (PRL)-IR cells (41.5±3.8%; 13.5±1.7%, respectively). In Dlk1−/− mice, adipocyte cells showed a significant increase in the TSH receptor mRNA expression level. Moreover, the percentage of LEPR-IR GH cells showed a statistically significant increase compared to controls, from 41.5±3.8% to 53.1±4.0%. By contrast, only 3.0±0.6% of LEP-IR anterior pituitary cells were detected in Dlk1 KO mice, as opposed to 6.8±1.1% observed in WT mice. The results suggest that relationships exist between adipocytes and pituitary GH, PRL and TSH cells, in addition to an influence with respect to the synthesis and release of pituitary leptin, particularly in PRL cells.Wiley201720172017info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10578/15491reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/154912026-05-27T07:36:41Z
dc.title.none.fl_str_mv Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
title Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
spellingShingle Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
Rodríguez Bello, Aixa
Anterior pituitary
Hormone receptors
Leptin receptor
Leptin
title_short Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
title_full Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
title_fullStr Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
title_full_unstemmed Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
title_sort Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
dc.creator.none.fl_str_mv Rodríguez Bello, Aixa
Puertas-Avendaño, Ricardo A.
González-Gómez, María Julia
González-Gómez, Miriam
Laborda, Jorge
Damas, Carmen
Ruiz-Hidalgo, María José
Díaz Delgado, Carmen
author Rodríguez Bello, Aixa
author_facet Rodríguez Bello, Aixa
Puertas-Avendaño, Ricardo A.
González-Gómez, María Julia
González-Gómez, Miriam
Laborda, Jorge
Damas, Carmen
Ruiz-Hidalgo, María José
Díaz Delgado, Carmen
author_role author
author2 Puertas-Avendaño, Ricardo A.
González-Gómez, María Julia
González-Gómez, Miriam
Laborda, Jorge
Damas, Carmen
Ruiz-Hidalgo, María José
Díaz Delgado, Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anterior pituitary
Hormone receptors
Leptin receptor
Leptin
topic Anterior pituitary
Hormone receptors
Leptin receptor
Leptin
description With the aim of studying delta-like protein 1 (DLK1) with respect to the relationship between adipocyte leptin and adenohypophyseal hormones, we carried out an immunohistochemical study analysing the presence of receptors for these hormones in the pituitary and adipose cells of male wild-type (WT) mice (Dlk1+/+) compared to knockout (KO) mice (Dlk1−/−). The mRNA expression of these molecules was also determined using the reverse transcriptase-polymerase chain reaction. The results obtained showed that, in WT adipose cells, all of the adenohypophyseal hormone receptors were present, with a higher mRNA expression for growth hormone (GH) receptor and thyroid-stimulating hormone (TSH) receptor. Of the total cells in the anterior pituitary lobe, 17.09±0.9% were leptin receptor (LEPR) immunoreactive (-IR), mainly in GH-IR and prolactin (PRL)-IR cells (41.5±3.8%; 13.5±1.7%, respectively). In Dlk1−/− mice, adipocyte cells showed a significant increase in the TSH receptor mRNA expression level. Moreover, the percentage of LEPR-IR GH cells showed a statistically significant increase compared to controls, from 41.5±3.8% to 53.1±4.0%. By contrast, only 3.0±0.6% of LEP-IR anterior pituitary cells were detected in Dlk1 KO mice, as opposed to 6.8±1.1% observed in WT mice. The results suggest that relationships exist between adipocytes and pituitary GH, PRL and TSH cells, in addition to an influence with respect to the synthesis and release of pituitary leptin, particularly in PRL cells.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10578/15491
url http://hdl.handle.net/10578/15491
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412078865874944
score 15.300719

Similares en LA Referencia