Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries

Tesis por compendio de publicaciones

Detalles Bibliográficos
Autor: Pereira, Eliana Andreia Pires Castilho
Tipo de recurso: tesis doctoral
Fecha de publicación:2016
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/133016
Acceso en línea:http://hdl.handle.net/10366/133016
Access Level:acceso abierto
Palabra clave:PHARMACY
Tesis y disertaciones académicas
Universidad de Salamanca (España)
Tesis Doctoral
Academic dissertations
Farmacología
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spelling Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industriesPereira, Eliana Andreia Pires CastilhoPHARMACYTesis y disertaciones académicasUniversidad de Salamanca (España)Tesis DoctoralAcademic dissertationsFarmacologíaTesis por compendio de publicaciones[EN] Medicinal plants and their extracts or isolated compounds have various applications, especially as food additives and as health promoters, as nutraceuticals and ingredients in formulations of functional foods, for pharmaceutical and food industries. However, medicinal plants may be contaminated by soil, water, air and powder, not only during the growth process but also during harvest and drying. Microbial contaminations reduce their quality and shelf life and pose a threat to public health, as they may involve the presence of pathogenic bacteria. Irradiation is a simple, modern and clean physical non-thermal method of processing that allows significant reduction in the microbial load, which is being increasingly applied as a feasible technology for different purposes: disinfestation, decontamination, sterilization or shelf-life extension of food products. It is authorized in several countries, including the European Union (Directive 1999/2/EC). In the present work, the feasibility of using gamma and e-beam (EB) radiation for preservation and decontamination of several aromatic and medicinal dried plants was evaluated, verifying that the main physico-chemical characteristics and relevant molecules were, in general, satisfactorily preserved, as also microbiological safety. The nutritional value was determined by official methodologies for food analysis; free sugars were analysed by HPLC-RI, fatty acids by GC-FID, organic acids by UFLC-DAD, tocopherols by HPLC-fluorescence, and phenolic compounds by HPLC-DAD-ESI/MS. The antioxidant properties were evaluated through free radicals scavenging activity, reducing power and inhibition of lipid peroxidation in brain homogenates assays. Cytotoxic properties were tested in tumor (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cell lines. Mycotoxin levels were determined by HPLC-fluorescence after immunoaffinity column (IAC) cleanup. Irradiation proved to produce heterogeneous effects on chemical, nutritional and bioactive variables, preserving or degrading molecules, and also increasing the bioavailability of some compounds. The preservation of sensible compounds over time (12 to 18 months) was not observed equally in all species submitted to irradiation, however, in general, EB stood out as the best processing option for the studied materials.Ferreira, Isabel Cristina Fernandes RodriguesSantos Buelga, CelestinoBento, Albino António201720172016info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10366/133016reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)Inglésinfo:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1330162026-06-07T06:28:51Z
dc.title.none.fl_str_mv Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
title Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
spellingShingle Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
Pereira, Eliana Andreia Pires Castilho
PHARMACY
Tesis y disertaciones académicas
Universidad de Salamanca (España)
Tesis Doctoral
Academic dissertations
Farmacología
title_short Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
title_full Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
title_fullStr Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
title_full_unstemmed Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
title_sort Evaluation of the effects of radiation on the chemical composition and bioactivity of the plants used in the pharmaceutical and/or food industries
dc.creator.none.fl_str_mv Pereira, Eliana Andreia Pires Castilho
author Pereira, Eliana Andreia Pires Castilho
author_facet Pereira, Eliana Andreia Pires Castilho
author_role author
dc.contributor.none.fl_str_mv Ferreira, Isabel Cristina Fernandes Rodrigues
Santos Buelga, Celestino
Bento, Albino António
dc.subject.none.fl_str_mv PHARMACY
Tesis y disertaciones académicas
Universidad de Salamanca (España)
Tesis Doctoral
Academic dissertations
Farmacología
topic PHARMACY
Tesis y disertaciones académicas
Universidad de Salamanca (España)
Tesis Doctoral
Academic dissertations
Farmacología
description Tesis por compendio de publicaciones
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/133016
url http://hdl.handle.net/10366/133016
dc.language.none.fl_str_mv
Inglés
language_invalid_str_mv
Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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