Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3

Unveiling the protective immune response to visceral leishmaniasis is critical for a rational design of vaccines aimed at reducing the impact caused by this fatal, if left untreated, vector-borne disease. In this study we sought to determine the role of the basic leucine zipper transcription factor...

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Detalhes bibliográficos
Autores: Soto, Manuel, Ramírez, Laura, Solana, José Carlos, Cook, Emma C. L., Hernández-García, Elena, Charro-Zanca, Sara, Redondo-Urzainqui, Ana, Reguera, Rosa M., Balaña-Fouce, Rafael, Iborra, Salvador
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/238792
Acesso em linha:http://hdl.handle.net/10261/238792
Access Level:acceso abierto
Palavra-chave:Leishmania, Batf3
Visceral leishmaniasis
Dendritic cells
Batf3 DC+
Th1 responses
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spelling Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3Soto, ManuelRamírez, LauraSolana, José CarlosCook, Emma C. L.Hernández-García, ElenaCharro-Zanca, SaraRedondo-Urzainqui, AnaReguera, Rosa M.Balaña-Fouce, RafaelIborra, SalvadorLeishmania, Batf3Visceral leishmaniasisDendritic cellsBatf3 DC+Th1 responsesUnveiling the protective immune response to visceral leishmaniasis is critical for a rational design of vaccines aimed at reducing the impact caused by this fatal, if left untreated, vector-borne disease. In this study we sought to determine the role of the basic leucine zipper transcription factor ATF-like 3 (Batf3) in the evolution of infection with Leishmania infantum, the causative agent of human visceral leishmaniasis in the Mediterranean Basin and Latin America. For that, Batf3-deficient mice in C57BL/6 background were infected with an L. infantum strain expressing the luciferase gene. Bioluminescent imaging, as well as in vitro parasite titration, demonstrated that Batf3-deficient mice were unable to control hepatic parasitosis as opposed to wild-type C57BL/6 mice. The impaired microbicide capacities of L. infantum-infected macrophages from Batf3-deficient mice mainly correlated with a reduction of parasite-specific IFN-γ production. Our results reinforce the implication of Batf3 in the generation of type 1 immunity against infectious diseases.Ministerio de Ciencia e Innovación FISPI11/00095 and FISPI14/00366 (FEDER FUNDING) and the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). SI is funded by RYC-2016-19463 and RTI2018-343 094484-B-I00 from Ministerio de Ciencia e Innovación (FEDER FUNDING). Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSOMinisterio de Ciencia e Innovación (España)Instituto de Salud Carlos IIIFundación Ramón ArecesBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/238792reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fimmu.2020.590934Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2387922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
title Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
spellingShingle Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
Soto, Manuel
Leishmania, Batf3
Visceral leishmaniasis
Dendritic cells
Batf3 DC+
Th1 responses
title_short Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
title_full Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
title_fullStr Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
title_full_unstemmed Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
title_sort Resistance to Experimental Visceral Leishmaniasis in Mice Infected With Leishmania infantum Requires Batf3
dc.creator.none.fl_str_mv Soto, Manuel
Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández-García, Elena
Charro-Zanca, Sara
Redondo-Urzainqui, Ana
Reguera, Rosa M.
Balaña-Fouce, Rafael
Iborra, Salvador
author Soto, Manuel
author_facet Soto, Manuel
Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández-García, Elena
Charro-Zanca, Sara
Redondo-Urzainqui, Ana
Reguera, Rosa M.
Balaña-Fouce, Rafael
Iborra, Salvador
author_role author
author2 Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández-García, Elena
Charro-Zanca, Sara
Redondo-Urzainqui, Ana
Reguera, Rosa M.
Balaña-Fouce, Rafael
Iborra, Salvador
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Instituto de Salud Carlos III
Fundación Ramón Areces
Banco Santander
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Leishmania, Batf3
Visceral leishmaniasis
Dendritic cells
Batf3 DC+
Th1 responses
topic Leishmania, Batf3
Visceral leishmaniasis
Dendritic cells
Batf3 DC+
Th1 responses
description Unveiling the protective immune response to visceral leishmaniasis is critical for a rational design of vaccines aimed at reducing the impact caused by this fatal, if left untreated, vector-borne disease. In this study we sought to determine the role of the basic leucine zipper transcription factor ATF-like 3 (Batf3) in the evolution of infection with Leishmania infantum, the causative agent of human visceral leishmaniasis in the Mediterranean Basin and Latin America. For that, Batf3-deficient mice in C57BL/6 background were infected with an L. infantum strain expressing the luciferase gene. Bioluminescent imaging, as well as in vitro parasite titration, demonstrated that Batf3-deficient mice were unable to control hepatic parasitosis as opposed to wild-type C57BL/6 mice. The impaired microbicide capacities of L. infantum-infected macrophages from Batf3-deficient mice mainly correlated with a reduction of parasite-specific IFN-γ production. Our results reinforce the implication of Batf3 in the generation of type 1 immunity against infectious diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/238792
url http://hdl.handle.net/10261/238792
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fimmu.2020.590934
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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