Exploring viral contamination in urban groundwater and runoff

The reliance of the global population on urban aquifers is steadily increasing, and urban aquifers are susceptible to pathogenic contamination through sources such as sewer leakage or urban runoff. However, there is insufficient monitoring of groundwater quality in urban areas. In this study, quanti...

Descripción completa

Detalles Bibliográficos
Autores: Itarte, Marta, Forés Gil, Eva, Martínez-Puchol, Sandra, Scheiber, Laura, Vázquez Suñé, Enric, Bofill Mas, Silvia, Rusiñol Arantegui, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/224717
Acceso en línea:https://hdl.handle.net/2445/224717
Access Level:acceso abierto
Palabra clave:Hidrologia urbana
Microbiologia aquàtica
Urban hydrology
Water microbiology
id ES_82cbfecb1465cabab37cdf4ff3e1c332
oai_identifier_str oai:diposit.ub.edu:2445/224717
network_acronym_str ES
network_name_str España
repository_id_str
spelling Exploring viral contamination in urban groundwater and runoffItarte, MartaForés Gil, EvaMartínez-Puchol, SandraScheiber, LauraVázquez Suñé, EnricBofill Mas, SilviaRusiñol Arantegui, MartaHidrologia urbanaMicrobiologia aquàticaUrban hydrologyWater microbiologyThe reliance of the global population on urban aquifers is steadily increasing, and urban aquifers are susceptible to pathogenic contamination through sources such as sewer leakage or urban runoff. However, there is insufficient monitoring of groundwater quality in urban areas. In this study, quantitative polymerase chain reaction (qPCR) was employed to evaluate the presence of human fecal viral indicators and viral pathogens in urban wastewater (n = 13) and groundwater (n = 12) samples from four locations in Barcelona with different degrees of urbanization, as well as in runoff samples (n = 2). Additionally, a target enrichment sequencing (TES) approach was utilized to explore the viral diversity within groundwater and runoff samples, offering insights into viral contamination and potential virus transmission routes in urban areas. Human adenovirus (HAdV) was identified in all wastewater samples, 67 % (8/12) of groundwater samples, and one runoff sample by qPCR indicating human viral fecal contamination. The viral pathogen Norovirus genogroup GI (NoV GI) was detected in wastewater and two winter groundwater samples from highly and medium urbanized areas. NoV genogroup GII (NoV GII), Enterovirus (EV) and SARS-CoV-2 were exclusively detected in wastewater. Human and other vertebrate viruses were detected in groundwater and runoff samples using TES. This study gives insights about the virome present in urban water sources, emphasizing the need for thorough monitoring and deeper understanding to address emerging public health concerns.Elsevier B.V.2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/224717Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2024.174238Science of the Total Environment, 2024, vol. 946https://doi.org/10.1016/j.scitotenv.2024.174238cc-by-nc-nd (c) Itarte, Marta et al., 2024http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2247172026-05-27T06:46:51Z
dc.title.none.fl_str_mv Exploring viral contamination in urban groundwater and runoff
title Exploring viral contamination in urban groundwater and runoff
spellingShingle Exploring viral contamination in urban groundwater and runoff
Itarte, Marta
Hidrologia urbana
Microbiologia aquàtica
Urban hydrology
Water microbiology
title_short Exploring viral contamination in urban groundwater and runoff
title_full Exploring viral contamination in urban groundwater and runoff
title_fullStr Exploring viral contamination in urban groundwater and runoff
title_full_unstemmed Exploring viral contamination in urban groundwater and runoff
title_sort Exploring viral contamination in urban groundwater and runoff
dc.creator.none.fl_str_mv Itarte, Marta
Forés Gil, Eva
Martínez-Puchol, Sandra
Scheiber, Laura
Vázquez Suñé, Enric
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
author Itarte, Marta
author_facet Itarte, Marta
Forés Gil, Eva
Martínez-Puchol, Sandra
Scheiber, Laura
Vázquez Suñé, Enric
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
author_role author
author2 Forés Gil, Eva
Martínez-Puchol, Sandra
Scheiber, Laura
Vázquez Suñé, Enric
Bofill Mas, Silvia
Rusiñol Arantegui, Marta
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hidrologia urbana
Microbiologia aquàtica
Urban hydrology
Water microbiology
topic Hidrologia urbana
Microbiologia aquàtica
Urban hydrology
Water microbiology
description The reliance of the global population on urban aquifers is steadily increasing, and urban aquifers are susceptible to pathogenic contamination through sources such as sewer leakage or urban runoff. However, there is insufficient monitoring of groundwater quality in urban areas. In this study, quantitative polymerase chain reaction (qPCR) was employed to evaluate the presence of human fecal viral indicators and viral pathogens in urban wastewater (n = 13) and groundwater (n = 12) samples from four locations in Barcelona with different degrees of urbanization, as well as in runoff samples (n = 2). Additionally, a target enrichment sequencing (TES) approach was utilized to explore the viral diversity within groundwater and runoff samples, offering insights into viral contamination and potential virus transmission routes in urban areas. Human adenovirus (HAdV) was identified in all wastewater samples, 67 % (8/12) of groundwater samples, and one runoff sample by qPCR indicating human viral fecal contamination. The viral pathogen Norovirus genogroup GI (NoV GI) was detected in wastewater and two winter groundwater samples from highly and medium urbanized areas. NoV genogroup GII (NoV GII), Enterovirus (EV) and SARS-CoV-2 were exclusively detected in wastewater. Human and other vertebrate viruses were detected in groundwater and runoff samples using TES. This study gives insights about the virome present in urban water sources, emphasizing the need for thorough monitoring and deeper understanding to address emerging public health concerns.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/224717
url https://hdl.handle.net/2445/224717
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.scitotenv.2024.174238
Science of the Total Environment, 2024, vol. 946
https://doi.org/10.1016/j.scitotenv.2024.174238
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Itarte, Marta et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Itarte, Marta et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412072509407233
score 15,812429

Similares en LA Referencia