A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction

We have established for the first time a mouse model of cannabinoid addiction using WIN 55,212-2 intravenous self-administration (0.0125 mg/kg/infusion) in C57Bl/6J mice. This model allows to evaluate the addiction criteria by grouping them into 1) persistence of response during a period of non-avai...

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Autores: Cajiao-Manrique, María del Mar, Maldonado, Rafael|||0000-0002-4359-8773, Martín-García, Elena|||0000-0002-4487-3028
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:275134
Acceso en línea:https://ddd.uab.cat/record/275134
https://dx.doi.org/urn:doi:10.3389/fphar.2023.1143365
Access Level:acceso abierto
Palabra clave:Cannabinoid addiction
Mouse model
WIN 55,212-2 self-administration
Persistence of response
Motivation
Compulsive-like behavior
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spelling A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addictionCajiao-Manrique, María del MarMaldonado, Rafael|||0000-0002-4359-8773Martín-García, Elena|||0000-0002-4487-3028Cannabinoid addictionMouse modelWIN 55,212-2 self-administrationPersistence of responseMotivationCompulsive-like behaviorWe have established for the first time a mouse model of cannabinoid addiction using WIN 55,212-2 intravenous self-administration (0.0125 mg/kg/infusion) in C57Bl/6J mice. This model allows to evaluate the addiction criteria by grouping them into 1) persistence of response during a period of non-availability of the drug, 2) motivation for WIN 55,212-2 with a progressive ratio, and 3) compulsivity when the reward is associated with a punishment such as an electric foot-shock, in agreement with the Diagnostic and Statistical Manual of Mental Disorders 5th edition (DSM-5). This model also allows to measure two parameters that have been related with the DSM-5 diagnostic criteria of craving, resistance to extinction and reinstatement, and two phenotypic traits suggested as predisposing factors, impulsivity and sensitivity to reward. We found that 35.6% of mice developed the criteria of cannabinoid addiction, allowing to differentiate between resilient and vulnerable mice. Therefore, we have established a novel and reliable model to study the neurobiological correlates underlying the resilience or vulnerability to develop cannabinoid addiction. This model included the chemogenetic inhibition of neuronal activity in the medial prefrontal cortex to the nucleus accumbens pathway to assess the neurobiological substrate of cannabinoid addiction. This model will shed light on the neurobiological substrate underlying cannabinoid addiction. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/275134https://dx.doi.org/urn:doi:10.3389/fphar.2023.1143365reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-120029GB-I00Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD21/0009/0019European Commission https://doi.org/10.13039/501100000780 848099European Commission https://doi.org/10.13039/501100000780 848068Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0017/0020Ministerio de Sanidad, Servicios Sociales e Igualdad https://doi.org/10.13039/501100003751 PNSD-2021I076Ministerio de Sanidad, Servicios Sociales e Igualdad https://doi.org/10.13039/501100003751 PNSD-2019I006Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2021-122073-2AAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-669"la Caixa" Foundation https://doi.org/10.13039/100010434 LCF/PR/HR22/52420017open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2751342026-06-06T12:50:31Z
dc.title.none.fl_str_mv A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
title A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
spellingShingle A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
Cajiao-Manrique, María del Mar
Cannabinoid addiction
Mouse model
WIN 55,212-2 self-administration
Persistence of response
Motivation
Compulsive-like behavior
title_short A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
title_full A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
title_fullStr A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
title_full_unstemmed A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
title_sort A male mouse model of WIN 55,212-2 self-administration to study cannabinoid addiction
dc.creator.none.fl_str_mv Cajiao-Manrique, María del Mar
Maldonado, Rafael|||0000-0002-4359-8773
Martín-García, Elena|||0000-0002-4487-3028
author Cajiao-Manrique, María del Mar
author_facet Cajiao-Manrique, María del Mar
Maldonado, Rafael|||0000-0002-4359-8773
Martín-García, Elena|||0000-0002-4487-3028
author_role author
author2 Maldonado, Rafael|||0000-0002-4359-8773
Martín-García, Elena|||0000-0002-4487-3028
author2_role author
author
dc.subject.none.fl_str_mv Cannabinoid addiction
Mouse model
WIN 55,212-2 self-administration
Persistence of response
Motivation
Compulsive-like behavior
topic Cannabinoid addiction
Mouse model
WIN 55,212-2 self-administration
Persistence of response
Motivation
Compulsive-like behavior
description We have established for the first time a mouse model of cannabinoid addiction using WIN 55,212-2 intravenous self-administration (0.0125 mg/kg/infusion) in C57Bl/6J mice. This model allows to evaluate the addiction criteria by grouping them into 1) persistence of response during a period of non-availability of the drug, 2) motivation for WIN 55,212-2 with a progressive ratio, and 3) compulsivity when the reward is associated with a punishment such as an electric foot-shock, in agreement with the Diagnostic and Statistical Manual of Mental Disorders 5th edition (DSM-5). This model also allows to measure two parameters that have been related with the DSM-5 diagnostic criteria of craving, resistance to extinction and reinstatement, and two phenotypic traits suggested as predisposing factors, impulsivity and sensitivity to reward. We found that 35.6% of mice developed the criteria of cannabinoid addiction, allowing to differentiate between resilient and vulnerable mice. Therefore, we have established a novel and reliable model to study the neurobiological correlates underlying the resilience or vulnerability to develop cannabinoid addiction. This model included the chemogenetic inhibition of neuronal activity in the medial prefrontal cortex to the nucleus accumbens pathway to assess the neurobiological substrate of cannabinoid addiction. This model will shed light on the neurobiological substrate underlying cannabinoid addiction.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/275134
https://dx.doi.org/urn:doi:10.3389/fphar.2023.1143365
url https://ddd.uab.cat/record/275134
https://dx.doi.org/urn:doi:10.3389/fphar.2023.1143365
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-120029GB-I00
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD21/0009/0019
European Commission https://doi.org/10.13039/501100000780 848099
European Commission https://doi.org/10.13039/501100000780 848068
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 RD16/0017/0020
Ministerio de Sanidad, Servicios Sociales e Igualdad https://doi.org/10.13039/501100003751 PNSD-2021I076
Ministerio de Sanidad, Servicios Sociales e Igualdad https://doi.org/10.13039/501100003751 PNSD-2019I006
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2021-122073-2A
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-669
"la Caixa" Foundation https://doi.org/10.13039/100010434 LCF/PR/HR22/52420017
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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