Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line

There has been an increase in the use of botanicals as skin photoprotective agents. Pomegranate (Punica granatum L.) is well known for its high concentration of polyphenolic compounds and for its antioxidant and anti-inflammatory properties. The aim of this study was to analyse the photoprotection p...

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Autores: Baccarin, Thaisa, Mitjans Arnal, Montserrat, Ramos López, David, Lemos-Senna, Elenara, Vinardell Martínez-Hidalgo, Ma. Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/96620
Acceso en línea:https://hdl.handle.net/2445/96620
Access Level:acceso abierto
Palabra clave:Litràcies
Olis vegetals
Emulsions (Farmàcia)
Protectors solars
Lythraceae
Vegetable oils
Emulsions (Pharmacy)
Sunscreens (Cosmetics)
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network_name_str España
repository_id_str
spelling Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell lineBaccarin, ThaisaMitjans Arnal, MontserratRamos López, DavidLemos-Senna, ElenaraVinardell Martínez-Hidalgo, Ma. PilarLitràciesOlis vegetalsEmulsions (Farmàcia)Protectors solarsLythraceaeVegetable oilsEmulsions (Pharmacy)Sunscreens (Cosmetics)There has been an increase in the use of botanicals as skin photoprotective agents. Pomegranate (Punica granatum L.) is well known for its high concentration of polyphenolic compounds and for its antioxidant and anti-inflammatory properties. The aim of this study was to analyse the photoprotection provided by Punica granatum seed oil nanoemulsion entrapping the polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in the keratinocyte HaCaT cell line. For this purpose, HaCaT cells were pretreated for 1 h with nanoemulsions in a serum-free medium and then irradiated with UVB (90<br>200 mJ/cm2) rays. Fluorescence microscopy analysis provided information about the cellular internalization of the nanodroplets. We also determined the in vitro SPF of the nanoemulsions and evaluated their phototoxicity using the 3T3 Neutral Red Uptake Phototoxicity Test. The nanoemulsions were able to protect the cells' DNA against UVB-induced damage in a concentration dependent manner. Nanodroplets were internalized by the cells but a higher proportion was detected along the cell membrane. The SPF obtained (~25) depended on the concentration of the ethyl acetate fraction and pomegranate seed oil in the nanoemulsion. The photoprotective formulations were classified as non-phototoxic. In conclusion, nanoemulsions entrapping the polyphenol-rich ethyl acetate fraction show potential for use as a sunscreen product.Elsevier B.V.2016201720152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfhttps://hdl.handle.net/2445/96620Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: http://dx.doi.org/10.1016/j.jphotobiol.2015.09.005Journal of Photochemistry and Photobiology B-Biology, 2015, vol. 153, p. 127-136http://dx.doi.org/10.1016/j.jphotobiol.2015.09.005cc-by-nc-nd (c) Elsevier B.V., 2015http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/966202026-05-29T05:05:01Z
dc.title.none.fl_str_mv Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
title Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
spellingShingle Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
Baccarin, Thaisa
Litràcies
Olis vegetals
Emulsions (Farmàcia)
Protectors solars
Lythraceae
Vegetable oils
Emulsions (Pharmacy)
Sunscreens (Cosmetics)
title_short Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
title_full Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
title_fullStr Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
title_full_unstemmed Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
title_sort Photoprotection by Punica granatum seed oil nanoemulsion entrapping polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in human keratinocyte (HaCaT) cell line
dc.creator.none.fl_str_mv Baccarin, Thaisa
Mitjans Arnal, Montserrat
Ramos López, David
Lemos-Senna, Elenara
Vinardell Martínez-Hidalgo, Ma. Pilar
author Baccarin, Thaisa
author_facet Baccarin, Thaisa
Mitjans Arnal, Montserrat
Ramos López, David
Lemos-Senna, Elenara
Vinardell Martínez-Hidalgo, Ma. Pilar
author_role author
author2 Mitjans Arnal, Montserrat
Ramos López, David
Lemos-Senna, Elenara
Vinardell Martínez-Hidalgo, Ma. Pilar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Litràcies
Olis vegetals
Emulsions (Farmàcia)
Protectors solars
Lythraceae
Vegetable oils
Emulsions (Pharmacy)
Sunscreens (Cosmetics)
topic Litràcies
Olis vegetals
Emulsions (Farmàcia)
Protectors solars
Lythraceae
Vegetable oils
Emulsions (Pharmacy)
Sunscreens (Cosmetics)
description There has been an increase in the use of botanicals as skin photoprotective agents. Pomegranate (Punica granatum L.) is well known for its high concentration of polyphenolic compounds and for its antioxidant and anti-inflammatory properties. The aim of this study was to analyse the photoprotection provided by Punica granatum seed oil nanoemulsion entrapping the polyphenol-rich ethyl acetate fraction against UVB-induced DNA damage in the keratinocyte HaCaT cell line. For this purpose, HaCaT cells were pretreated for 1 h with nanoemulsions in a serum-free medium and then irradiated with UVB (90<br>200 mJ/cm2) rays. Fluorescence microscopy analysis provided information about the cellular internalization of the nanodroplets. We also determined the in vitro SPF of the nanoemulsions and evaluated their phototoxicity using the 3T3 Neutral Red Uptake Phototoxicity Test. The nanoemulsions were able to protect the cells' DNA against UVB-induced damage in a concentration dependent manner. Nanodroplets were internalized by the cells but a higher proportion was detected along the cell membrane. The SPF obtained (~25) depended on the concentration of the ethyl acetate fraction and pomegranate seed oil in the nanoemulsion. The photoprotective formulations were classified as non-phototoxic. In conclusion, nanoemulsions entrapping the polyphenol-rich ethyl acetate fraction show potential for use as a sunscreen product.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/96620
url https://hdl.handle.net/2445/96620
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.jphotobiol.2015.09.005
Journal of Photochemistry and Photobiology B-Biology, 2015, vol. 153, p. 127-136
http://dx.doi.org/10.1016/j.jphotobiol.2015.09.005
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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