Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency

Producción Científica

Detalles Bibliográficos
Autores: Galindo, Sara, López Paniagua, Marina, De La Mata Sampedro, Ana, Herreras Cantalapiedra, José María, García Vázquez, Carmen, Marceñido, Beatriz, Rey, Esther, Higuera Barón, Celia, Calonge, Margarita, Nieto Miguel, Teresa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/80765
Acceso en línea:https://doi.org/10.1016/j.exer.2025.110737
https://uvadoc.uva.es/handle/10324/80765
Access Level:acceso abierto
Palabra clave:Cornea
Corneal epithelium
Limbus
Limbal stem cell deficiency
Mesenchymal stem cells
Regeneration
Stem cell transplantation
3201.09 Oftalmología
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spelling Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiencyGalindo, SaraLópez Paniagua, MarinaDe La Mata Sampedro, AnaHerreras Cantalapiedra, José MaríaGarcía Vázquez, CarmenMarceñido, BeatrizRey, EstherHiguera Barón, CeliaCalonge, MargaritaNieto Miguel, TeresaCorneaCorneal epitheliumLimbusLimbal stem cell deficiencyMesenchymal stem cellsRegenerationStem cell transplantation3201.09 OftalmologíaProducción CientíficaOcular limbal stem cell deficiency (LSCD) occurs because of corneal epithelial stem cell destruction or dysfunction at the limbal niche. LSCD can cause corneal blindness, and the current therapy based on limbal stem cell transplantation is continuously improving. The aim of this work was to compare the safety and efficacy of human mesenchymal stem cells (hMSCs) derived from bone marrow (hBM-MSCs) and adipose tissue (hAT-MSCs) when transplanted to a rabbit model of LSCD. Both hMSC types expressed the corneal and limbal epithelial cell markers CK3, CK12, ZO-1, and ABCG2 under standard culture conditions. A few hBM-MSCs expressed CK7 and E- cadherin, while hAT-MSCs expressed more CK7 but no E-cadherin. The hMSCs were seeded onto amniotic membranes and transplanted onto the ocular surface of a LSCD rabbit model. Both hMSC types were well tolerated without immunosuppression and were primarily located in the superior limbal stroma eight weeks post- transplantation. The hBM-MSC–treated group showed less superficial neovascularization, while the hAT- MSC–treated group showed less conjunctival invasion and fewer corneal stromal blood vessels. Compared to the untreated LSCD group, both hMSC-treated groups had less corneal opacity, less corneal and limbal stromal inflammation, and more corneal epithelial layers that partially recovered the corneal and limbal epithelial markers CK3, CK15, and p63. Overall, transplantation of hBM-MSCs and hAT-MSCs in a rabbit LSCD model reduced the development of corneal opacity, neovascularization, inflammation, and partially restored corneal and limbal tissue structure and epithelial cell phenotypes. Therefore, both types of hMSCs could become valid alternatives for LSCD treatment.Ministerio de Economía y Competitividad y el Fondo Europeo de Desarrollo Regional, España (subvención SAF2015-63594-R MINECO/FEDER, UE)Ministerio de Ciencia e Innovación (subvención PID2019-105525RB-100 AEI/10.13039/501100011033)Instituto Nacional de Salud Carlos III, CIBER-BBN (CB06/01/003 MICINN/FEDER, UE)Junta de Castilla y León (Consejería de Educación) y el Fondo Europeo de Desarrollo Regional (subvención CLU-2023-1-04)Elsevier2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.exer.2025.110737https://uvadoc.uva.es/handle/10324/80765reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S001448352500510Xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/807652026-06-13T12:44:47Z
dc.title.none.fl_str_mv Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
title Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
spellingShingle Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
Galindo, Sara
Cornea
Corneal epithelium
Limbus
Limbal stem cell deficiency
Mesenchymal stem cells
Regeneration
Stem cell transplantation
3201.09 Oftalmología
title_short Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
title_full Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
title_fullStr Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
title_full_unstemmed Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
title_sort Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
dc.creator.none.fl_str_mv Galindo, Sara
López Paniagua, Marina
De La Mata Sampedro, Ana
Herreras Cantalapiedra, José María
García Vázquez, Carmen
Marceñido, Beatriz
Rey, Esther
Higuera Barón, Celia
Calonge, Margarita
Nieto Miguel, Teresa
author Galindo, Sara
author_facet Galindo, Sara
López Paniagua, Marina
De La Mata Sampedro, Ana
Herreras Cantalapiedra, José María
García Vázquez, Carmen
Marceñido, Beatriz
Rey, Esther
Higuera Barón, Celia
Calonge, Margarita
Nieto Miguel, Teresa
author_role author
author2 López Paniagua, Marina
De La Mata Sampedro, Ana
Herreras Cantalapiedra, José María
García Vázquez, Carmen
Marceñido, Beatriz
Rey, Esther
Higuera Barón, Celia
Calonge, Margarita
Nieto Miguel, Teresa
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cornea
Corneal epithelium
Limbus
Limbal stem cell deficiency
Mesenchymal stem cells
Regeneration
Stem cell transplantation
3201.09 Oftalmología
topic Cornea
Corneal epithelium
Limbus
Limbal stem cell deficiency
Mesenchymal stem cells
Regeneration
Stem cell transplantation
3201.09 Oftalmología
description Producción Científica
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.exer.2025.110737
https://uvadoc.uva.es/handle/10324/80765
url https://doi.org/10.1016/j.exer.2025.110737
https://uvadoc.uva.es/handle/10324/80765
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S001448352500510X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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