First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

[EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of thi...

Descripción completa

Detalles Bibliográficos
Autores: Asturiano, Juan F.|||0000-0002-6441-5294, Pérez Igualada, Luz María|||0000-0002-6678-7446, Sorensen, S.R., Lauesen, P., Tomkiewicz, J.
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/104774
Acceso en línea:https://riunet.upv.es/handle/10251/104774
Access Level:acceso abierto
Palabra clave:Cryopreservation
Spermatozoa
Fertilization
Eel larvae
Anguilla anguilla
PRODUCCION ANIMAL
id ES_81d0bc6a00d9cf0e6f0a1c1588d85708
oai_identifier_str oai:riunet.upv.es:10251/104774
network_acronym_str ES
network_name_str España
repository_id_str
spelling First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacityAsturiano, Juan F.|||0000-0002-6441-5294Pérez Igualada, Luz María|||0000-0002-6678-7446Sorensen, S.R.Lauesen, P.Tomkiewicz, J.CryopreservationSpermatozoaFertilizationEel larvaeAnguilla anguillaPRODUCCION ANIMAL[EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. This study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested whether a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20 degrees C, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20 degrees C). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (cryolarvae') were obtained 55h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.This study was part of the project: 'Reproduction of European Eel: Towards a Self-sustained Aquaculture' (PRO-EEL) European Community's 7th Framework Programme under the Theme 2 'Food, Agriculture and Fisheries, and Biotechnology', Grant Agreement no245257. Juan F. Asturiano and Luz Perez had a grant to staying in Denmark from Programa de Apoyo a la Investigacion y Desarrollo (PAID-00-11) of the Universitat Politecnica de Valencia. We thank Christian Graver and Lars B. Tybjerg for assistance duringBlackwell PublishingDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/104774reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 FP7 245257 Reproduction of European Eel: Towards a Self-sustained Aquacultureopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1047742026-06-13T07:49:27Z
dc.title.none.fl_str_mv First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
title First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
spellingShingle First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
Asturiano, Juan F.|||0000-0002-6441-5294
Cryopreservation
Spermatozoa
Fertilization
Eel larvae
Anguilla anguilla
PRODUCCION ANIMAL
title_short First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
title_full First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
title_fullStr First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
title_full_unstemmed First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
title_sort First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity
dc.creator.none.fl_str_mv Asturiano, Juan F.|||0000-0002-6441-5294
Pérez Igualada, Luz María|||0000-0002-6678-7446
Sorensen, S.R.
Lauesen, P.
Tomkiewicz, J.
author Asturiano, Juan F.|||0000-0002-6441-5294
author_facet Asturiano, Juan F.|||0000-0002-6441-5294
Pérez Igualada, Luz María|||0000-0002-6678-7446
Sorensen, S.R.
Lauesen, P.
Tomkiewicz, J.
author_role author
author2 Pérez Igualada, Luz María|||0000-0002-6678-7446
Sorensen, S.R.
Lauesen, P.
Tomkiewicz, J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Ciencia Animal
Instituto Universitario de Ciencia y Tecnología Animal
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
European Commission
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Cryopreservation
Spermatozoa
Fertilization
Eel larvae
Anguilla anguilla
PRODUCCION ANIMAL
topic Cryopreservation
Spermatozoa
Fertilization
Eel larvae
Anguilla anguilla
PRODUCCION ANIMAL
description [EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. This study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested whether a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20 degrees C, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20 degrees C). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (cryolarvae') were obtained 55h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/104774
url https://riunet.upv.es/handle/10251/104774
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 FP7 245257 Reproduction of European Eel: Towards a Self-sustained Aquaculture
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412001459994625
score 15,301603

Similares en LA Referencia