Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus
The brucellae are facultative intracellular pathogens of mammals that are transmitted by contact with infected animals or contaminated materials. Several major lipidic components of the brucella cell envelope are imperfectly recognized by innate immunity, thus contributing to virulence. These compon...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/29633 |
| Acceso en línea: | https://hdl.handle.net/10171/29633 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacterial proteins metabolism Brucella abortus enzymology Cyclopropanes metabolism Fatty acids metabolism Methyltransferases metabolism Female |
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Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortusPalacios-Chaves, L. (Leyre)|||/items/1d32ef4f-a851-47eb-81bd-4988530d4c50Zuñiga-Ripa, A. (Amaia)|||/items/9aa6da2e-e507-4319-a12c-092436f70febGutierrez, A. (Ana)|||/items/0581ba9a-d0f2-4374-bf7c-1723eeedcc22Gil-Ramirez, Y. (Yolanda)|||/items/f834f1d2-8f4c-4ad8-ae9b-53314fbe1eb0Conde-Álvarez, R. (Raquel)|||/items/7a805559-3962-434b-9df9-ec243d61b5aaMoriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7bIriarte-Cilveti, M. (Maite)|||/items/5b20b123-3967-4f07-b577-13d13aa57363Bacterial proteins metabolismBrucella abortus enzymologyCyclopropanes metabolismFatty acids metabolismMethyltransferases metabolismFemaleThe brucellae are facultative intracellular pathogens of mammals that are transmitted by contact with infected animals or contaminated materials. Several major lipidic components of the brucella cell envelope are imperfectly recognized by innate immunity, thus contributing to virulence. These components carry large proportions of acyl chains of lactobacillic acid, a long chain cyclopropane fatty acid (CFA). CFAs result from addition of a methylene group to unsaturated acyl chains and contribute to resistance to acidity, dryness and high osmolarity in many bacteria and to virulence in mycobacteria. We examined the role of lactobacillic acid in Brucella abortus virulence by creating a mutant in ORF BAB1_0476, the putative CFA synthase gene. The mutant did not incorporate [(14)C]methyl groups into lipids, lacked CFAs and synthesized the unsaturated precursors, proving that BAB1_0476 actually encodes a CFA synthase. BAB1_0476 promoter-luxAB fusion studies showed that CFA synthase expression was promoted by acid pH and high osmolarity. The mutant was not attenuated in macrophages or mice, strongly suggesting that CFAs are not essential for B. abortus intracellular life. However, when the mutant was tested under high osmolarity on agar and acid pH, two conditions likely to occur on contaminated materials and fomites, they showed reduced ability to grow or survive. Since CFA synthesis entails high ATP expenses and brucellae produce large proportions of lactobacillic acyl chains, we speculate that the CFA synthase has been conserved because it is useful for survival extracellularly, thus facilitating persistence in contaminated materials and transmission to new hosts.Society for General MicrobiologyDadun. Depósito Académico Digital Universidad de Navarra20132013-08-3020122012-01-0120122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/29633reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/296332026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| title |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| spellingShingle |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus Palacios-Chaves, L. (Leyre)|||/items/1d32ef4f-a851-47eb-81bd-4988530d4c50 Bacterial proteins metabolism Brucella abortus enzymology Cyclopropanes metabolism Fatty acids metabolism Methyltransferases metabolism Female |
| title_short |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| title_full |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| title_fullStr |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| title_full_unstemmed |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| title_sort |
Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus |
| dc.creator.none.fl_str_mv |
Palacios-Chaves, L. (Leyre)|||/items/1d32ef4f-a851-47eb-81bd-4988530d4c50 Zuñiga-Ripa, A. (Amaia)|||/items/9aa6da2e-e507-4319-a12c-092436f70feb Gutierrez, A. (Ana)|||/items/0581ba9a-d0f2-4374-bf7c-1723eeedcc22 Gil-Ramirez, Y. (Yolanda)|||/items/f834f1d2-8f4c-4ad8-ae9b-53314fbe1eb0 Conde-Álvarez, R. (Raquel)|||/items/7a805559-3962-434b-9df9-ec243d61b5aa Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b Iriarte-Cilveti, M. (Maite)|||/items/5b20b123-3967-4f07-b577-13d13aa57363 |
| author |
Palacios-Chaves, L. (Leyre)|||/items/1d32ef4f-a851-47eb-81bd-4988530d4c50 |
| author_facet |
Palacios-Chaves, L. (Leyre)|||/items/1d32ef4f-a851-47eb-81bd-4988530d4c50 Zuñiga-Ripa, A. (Amaia)|||/items/9aa6da2e-e507-4319-a12c-092436f70feb Gutierrez, A. (Ana)|||/items/0581ba9a-d0f2-4374-bf7c-1723eeedcc22 Gil-Ramirez, Y. (Yolanda)|||/items/f834f1d2-8f4c-4ad8-ae9b-53314fbe1eb0 Conde-Álvarez, R. (Raquel)|||/items/7a805559-3962-434b-9df9-ec243d61b5aa Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b Iriarte-Cilveti, M. (Maite)|||/items/5b20b123-3967-4f07-b577-13d13aa57363 |
| author_role |
author |
| author2 |
Zuñiga-Ripa, A. (Amaia)|||/items/9aa6da2e-e507-4319-a12c-092436f70feb Gutierrez, A. (Ana)|||/items/0581ba9a-d0f2-4374-bf7c-1723eeedcc22 Gil-Ramirez, Y. (Yolanda)|||/items/f834f1d2-8f4c-4ad8-ae9b-53314fbe1eb0 Conde-Álvarez, R. (Raquel)|||/items/7a805559-3962-434b-9df9-ec243d61b5aa Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b Iriarte-Cilveti, M. (Maite)|||/items/5b20b123-3967-4f07-b577-13d13aa57363 |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Bacterial proteins metabolism Brucella abortus enzymology Cyclopropanes metabolism Fatty acids metabolism Methyltransferases metabolism Female |
| topic |
Bacterial proteins metabolism Brucella abortus enzymology Cyclopropanes metabolism Fatty acids metabolism Methyltransferases metabolism Female |
| description |
The brucellae are facultative intracellular pathogens of mammals that are transmitted by contact with infected animals or contaminated materials. Several major lipidic components of the brucella cell envelope are imperfectly recognized by innate immunity, thus contributing to virulence. These components carry large proportions of acyl chains of lactobacillic acid, a long chain cyclopropane fatty acid (CFA). CFAs result from addition of a methylene group to unsaturated acyl chains and contribute to resistance to acidity, dryness and high osmolarity in many bacteria and to virulence in mycobacteria. We examined the role of lactobacillic acid in Brucella abortus virulence by creating a mutant in ORF BAB1_0476, the putative CFA synthase gene. The mutant did not incorporate [(14)C]methyl groups into lipids, lacked CFAs and synthesized the unsaturated precursors, proving that BAB1_0476 actually encodes a CFA synthase. BAB1_0476 promoter-luxAB fusion studies showed that CFA synthase expression was promoted by acid pH and high osmolarity. The mutant was not attenuated in macrophages or mice, strongly suggesting that CFAs are not essential for B. abortus intracellular life. However, when the mutant was tested under high osmolarity on agar and acid pH, two conditions likely to occur on contaminated materials and fomites, they showed reduced ability to grow or survive. Since CFA synthesis entails high ATP expenses and brucellae produce large proportions of lactobacillic acyl chains, we speculate that the CFA synthase has been conserved because it is useful for survival extracellularly, thus facilitating persistence in contaminated materials and transmission to new hosts. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2012-01-01 2012 2012-01-01 2013 2013-08-30 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/29633 |
| url |
https://hdl.handle.net/10171/29633 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Society for General Microbiology |
| publisher.none.fl_str_mv |
Society for General Microbiology |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869411999606112256 |
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15,300719 |