Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade

[EN] Aldosterone administration in rats results in several cardiac alterations. Previous studies have demonstrated that proanthocyanidins, phenolic bioactive compounds, have cardioprotective effects. We studied the potential beneficial effects of the proanthocyanidin-rich almond skin extract (PASE)...

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Authors: Martín Fernández, Beatriz, Heras Jiménez, Natalia de las, Valero Muñoz, María, Ballesteros Rodrigálvarez, Sandra María, Yao, Yi-Zhou, Stanton, Peter Gordon, Fuller, Peter J., Lahera Juliá, Vicente
Format: article
Status:Published version
Publication Date:2014
Country:España
Institution:Universidad de León
Repository:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24575
Online Access:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111104
https://hdl.handle.net/10612/24575
Access Level:Open access
Keyword:Biología
Bioquímica
Medicina. Salud
Veterinaria
Aldosterone
Hypertension
Immediate-Early Proteins
Mineralocorticoid Receptor Antagonists
Plant Extracts
Proanthocyanidins
Cardiac hypertrophy
2407 Biología Celular
3209.07 Fitofármacos
3207.04 Patología Cardiovascular
id ES_81619d1ebb3a2cb3e411232cedfda3d7
oai_identifier_str oai:buleria.unileon.es:10612/24575
network_acronym_str ES
network_name_str España
repository_id_str
spelling Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor BlockadeMartín Fernández, BeatrizHeras Jiménez, Natalia de lasValero Muñoz, MaríaBallesteros Rodrigálvarez, Sandra MaríaYao, Yi-ZhouStanton, Peter GordonFuller, Peter J.Lahera Juliá, VicenteBiologíaBioquímicaMedicina. SaludVeterinariaAldosteroneHypertensionImmediate-Early ProteinsMineralocorticoid Receptor AntagonistsPlant ExtractsProanthocyanidinsCardiac hypertrophy2407 Biología Celular3209.07 Fitofármacos3207.04 Patología Cardiovascular[EN] Aldosterone administration in rats results in several cardiac alterations. Previous studies have demonstrated that proanthocyanidins, phenolic bioactive compounds, have cardioprotective effects. We studied the potential beneficial effects of the proanthocyanidin-rich almond skin extract (PASE) on the cardiac alterations induced by aldosterone-salt treatment, their effects in mineralocorticoid receptor activity and we sought to confirm proanthocyanidins as the specific component of the extract involved in the beneficial cardiac effects. Male Wistar rats received aldosterone (1 mg/Kg/day) +1% NaCl for 3 weeks. Half of the animals in each group were simultaneously treated with either PASE (100 mg/Kg/day) or spironolactone (200 mg/Kg/day). The ability of PASE to act as an antagonist of the mineralocorticoid receptor was examined using a transactivation assay. High performance liquid chromatography was used to identify and to isolate proanthocyanidins. Hypertension and diastolic dysfunction induced by aldosterone were abolished by treatment with PASE. Expression of the aldosterone mediator SGK-1, together with fibrotic, inflammatory and oxidative mediators were increased by aldosteronesalt treatment; these were reduced by PASE. Aldosterone-salt induced transcriptional activity of the mineralocorticoid receptor was reduced by PASE. HPLC confirmed proanthocyanidins as the compound responsible for the beneficial effects of PASE. The effects of PASE were comparable to those seen with the mineralocorticoid antagonist, spironolactone. The observed responses in the aldosterone-salt treated rats together with the antagonism of transactivation at the mineralocorticoid receptor by PASE provides evidence that the beneficial effect of this proanthocyanidin-rich almond skin extract is via as a mineralocorticoid receptor antagonist with proanthocyanidins identified as the compounds responsible for the beneficial effects of PASESIThis work was supported by grants from VI Programa Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica de España (SAF2011-30396) and the National Health and Medical Research Council of Australia through a Fellowship (#1002559) and Project Grant (#1002575) support to PJF. Prince Henry’s Institute is supported by the Victorian Government’s Operational Infrastructure ProgramPublic Library of ScienceBiologia CelularFacultad de Ciencias Biológicas y Ambientales2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111104https://hdl.handle.net/10612/24575reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/SAF2011-30396http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/245752026-06-24T12:43:27Z
dc.title.none.fl_str_mv Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
title Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
spellingShingle Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
Martín Fernández, Beatriz
Biología
Bioquímica
Medicina. Salud
Veterinaria
Aldosterone
Hypertension
Immediate-Early Proteins
Mineralocorticoid Receptor Antagonists
Plant Extracts
Proanthocyanidins
Cardiac hypertrophy
2407 Biología Celular
3209.07 Fitofármacos
3207.04 Patología Cardiovascular
title_short Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
title_full Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
title_fullStr Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
title_full_unstemmed Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
title_sort Beneficial Effects of Proanthocyanidins in the Cardiac Alterations Induced by Aldosterone in Rat Heart through Mineralocorticoid Receptor Blockade
dc.creator.none.fl_str_mv Martín Fernández, Beatriz
Heras Jiménez, Natalia de las
Valero Muñoz, María
Ballesteros Rodrigálvarez, Sandra María
Yao, Yi-Zhou
Stanton, Peter Gordon
Fuller, Peter J.
Lahera Juliá, Vicente
author Martín Fernández, Beatriz
author_facet Martín Fernández, Beatriz
Heras Jiménez, Natalia de las
Valero Muñoz, María
Ballesteros Rodrigálvarez, Sandra María
Yao, Yi-Zhou
Stanton, Peter Gordon
Fuller, Peter J.
Lahera Juliá, Vicente
author_role author
author2 Heras Jiménez, Natalia de las
Valero Muñoz, María
Ballesteros Rodrigálvarez, Sandra María
Yao, Yi-Zhou
Stanton, Peter Gordon
Fuller, Peter J.
Lahera Juliá, Vicente
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biológicas y Ambientales
dc.subject.none.fl_str_mv Biología
Bioquímica
Medicina. Salud
Veterinaria
Aldosterone
Hypertension
Immediate-Early Proteins
Mineralocorticoid Receptor Antagonists
Plant Extracts
Proanthocyanidins
Cardiac hypertrophy
2407 Biología Celular
3209.07 Fitofármacos
3207.04 Patología Cardiovascular
topic Biología
Bioquímica
Medicina. Salud
Veterinaria
Aldosterone
Hypertension
Immediate-Early Proteins
Mineralocorticoid Receptor Antagonists
Plant Extracts
Proanthocyanidins
Cardiac hypertrophy
2407 Biología Celular
3209.07 Fitofármacos
3207.04 Patología Cardiovascular
description [EN] Aldosterone administration in rats results in several cardiac alterations. Previous studies have demonstrated that proanthocyanidins, phenolic bioactive compounds, have cardioprotective effects. We studied the potential beneficial effects of the proanthocyanidin-rich almond skin extract (PASE) on the cardiac alterations induced by aldosterone-salt treatment, their effects in mineralocorticoid receptor activity and we sought to confirm proanthocyanidins as the specific component of the extract involved in the beneficial cardiac effects. Male Wistar rats received aldosterone (1 mg/Kg/day) +1% NaCl for 3 weeks. Half of the animals in each group were simultaneously treated with either PASE (100 mg/Kg/day) or spironolactone (200 mg/Kg/day). The ability of PASE to act as an antagonist of the mineralocorticoid receptor was examined using a transactivation assay. High performance liquid chromatography was used to identify and to isolate proanthocyanidins. Hypertension and diastolic dysfunction induced by aldosterone were abolished by treatment with PASE. Expression of the aldosterone mediator SGK-1, together with fibrotic, inflammatory and oxidative mediators were increased by aldosteronesalt treatment; these were reduced by PASE. Aldosterone-salt induced transcriptional activity of the mineralocorticoid receptor was reduced by PASE. HPLC confirmed proanthocyanidins as the compound responsible for the beneficial effects of PASE. The effects of PASE were comparable to those seen with the mineralocorticoid antagonist, spironolactone. The observed responses in the aldosterone-salt treated rats together with the antagonism of transactivation at the mineralocorticoid receptor by PASE provides evidence that the beneficial effect of this proanthocyanidin-rich almond skin extract is via as a mineralocorticoid receptor antagonist with proanthocyanidins identified as the compounds responsible for the beneficial effects of PASE
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111104
https://hdl.handle.net/10612/24575
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111104
https://hdl.handle.net/10612/24575
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/SAF2011-30396
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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