Osmoregulation in freshwater anaerobic methane-oxidizing archaea under salt stress
Climate change–driven sea level rise threatens freshwater ecosystems and elicits salinity stress in microbiomes. Methane emissions in these systems are largely mitigated by methane-oxidizing microorganisms. Here, we characterized the physiological and metabolic response of freshwater methanotrophic...
| Authors: | , , , , , , , , , , |
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| Format: | article |
| Publication Date: | 2024 |
| Country: | España |
| Institution: | IE |
| Repository: | Repositorio IE |
| OAI Identifier: | oai:repositorio.ie.edu:20.500.14417/4215 |
| Online Access: | https://doi.org/10.1093/ismejo/wrae137 https://hdl.handle.net/20.500.14417/4215 https://academic.oup.com/ismej/article/18/1/wrae137/7717430 |
| Access Level: | Open access |
| Keyword: | ODS 13 - Acción por el clima compatible solutes methanotroph salinity adaptation ANME metabolomics “Ca. Methanoperedens” |
| Summary: | Climate change–driven sea level rise threatens freshwater ecosystems and elicits salinity stress in microbiomes. Methane emissions in these systems are largely mitigated by methane-oxidizing microorganisms. Here, we characterized the physiological and metabolic response of freshwater methanotrophic archaea to salt stress. In our microcosm experiments, inhibition of methanotrophic archaea started at 1%. However, during gradual increase of salt up to 3% in a reactor over 12 weeks, the culture continued to oxidize methane. Using gene expression profiles and metabolomics, we identified a pathway for salt-stress response that produces the osmolyte of anaerobic methanotrophic archaea: N(ε)-acetyl-β-L-lysine. An extensive phylogenomic analysis on N(ε)-acetyl-β-L-lysine-producing enzymes revealed that they are widespread across both bacteria and archaea, indicating a potential horizontal gene transfer and a link to BORG extrachromosomal elements. Physicochemical analysis of bioreactor biomass further indicated the presence of sialic acids and the consumption of intracellular polyhydroxyalkanoates in anaerobic methanotrophs during salt stress. |
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