Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization

This study was supported by the Ministry of Science and Innovation (MICINN) and the Ministry of Economy and Competitiveness (MINECO) of the Spanish Government (Projects: MAT2009-12547, MAT2012-30706 co-funded by the European Union through European Regional Development Funds). C.M.-M. thanks the supp...

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Detalhes bibliográficos
Autores: Godoy-Gallardo, Maria, Mas-Moruno, Carlos, Yu, Kai, Manero, José María, Gil, FJ, Kizhakkedathu, Jayachandran, Rodríguez Rius, Daniel
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/2946
Acesso em linha:http://hdl.handle.net/20.500.12328/2946
http://dx.doi.org/10.1021/bm501528x
Access Level:acceso abierto
Palavra-chave:Materials de recobriment
Biofilms
Pèptids i proteïnes
Titani
Polimerització radical
Implants dentals
Materiales de revestimiento
Péptidos y proteínas
Titanio
Polimerización radical
Implantes dentales
Coating materials
Peptides and proteins
Titanium
Radical polymerization
Dental implants
616.3
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oai_identifier_str oai:recercat.cat:20.500.12328/2946
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repository_id_str
spelling Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerizationGodoy-Gallardo, MariaMas-Moruno, CarlosYu, KaiManero, José MaríaGil, FJKizhakkedathu, JayachandranRodríguez Rius, DanielMaterials de recobrimentBiofilmsPèptids i proteïnesTitaniPolimerització radicalImplants dentalsMateriales de revestimientoBiofilmsPéptidos y proteínasTitanioPolimerización radicalImplantes dentalesCoating materialsBiofilmsPeptides and proteinsTitaniumRadical polymerizationDental implants616.3This study was supported by the Ministry of Science and Innovation (MICINN) and the Ministry of Economy and Competitiveness (MINECO) of the Spanish Government (Projects: MAT2009-12547, MAT2012-30706 co-funded by the European Union through European Regional Development Funds). C.M.-M. thanks the support of the Secretary for Universities and Research of the Ministry of Economy and Knowledge of the Government of Catalonia (2011-BP-B-00042) and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7-PEOPLE-2012-CIG, REA grant agreement n° 321985).Dental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1–11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1–11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment.info:eu-repo/semantics/acceptedVersionACS Publications2015info:eu-repo/semantics/article13http://hdl.handle.net/20.500.12328/2946http://dx.doi.org/10.1021/bm501528xreponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBiomacromolecules2;info:eu-repo/grantAgreement/ES/MICINN/MAT2009-12547info:eu-repo/grantAgreement/ES/MINECO/MAT2012-30706info:eu-repo/grantAgreement/EC/FP7/321985© 2014 American Chemical Societyinfo:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/29462026-05-29T05:05:01Z
dc.title.none.fl_str_mv Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
title Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
spellingShingle Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
Godoy-Gallardo, Maria
Materials de recobriment
Biofilms
Pèptids i proteïnes
Titani
Polimerització radical
Implants dentals
Materiales de revestimiento
Biofilms
Péptidos y proteínas
Titanio
Polimerización radical
Implantes dentales
Coating materials
Biofilms
Peptides and proteins
Titanium
Radical polymerization
Dental implants
616.3
title_short Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
title_full Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
title_fullStr Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
title_full_unstemmed Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
title_sort Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
dc.creator.none.fl_str_mv Godoy-Gallardo, Maria
Mas-Moruno, Carlos
Yu, Kai
Manero, José María
Gil, FJ
Kizhakkedathu, Jayachandran
Rodríguez Rius, Daniel
author Godoy-Gallardo, Maria
author_facet Godoy-Gallardo, Maria
Mas-Moruno, Carlos
Yu, Kai
Manero, José María
Gil, FJ
Kizhakkedathu, Jayachandran
Rodríguez Rius, Daniel
author_role author
author2 Mas-Moruno, Carlos
Yu, Kai
Manero, José María
Gil, FJ
Kizhakkedathu, Jayachandran
Rodríguez Rius, Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Materials de recobriment
Biofilms
Pèptids i proteïnes
Titani
Polimerització radical
Implants dentals
Materiales de revestimiento
Biofilms
Péptidos y proteínas
Titanio
Polimerización radical
Implantes dentales
Coating materials
Biofilms
Peptides and proteins
Titanium
Radical polymerization
Dental implants
616.3
topic Materials de recobriment
Biofilms
Pèptids i proteïnes
Titani
Polimerització radical
Implants dentals
Materiales de revestimiento
Biofilms
Péptidos y proteínas
Titanio
Polimerización radical
Implantes dentales
Coating materials
Biofilms
Peptides and proteins
Titanium
Radical polymerization
Dental implants
616.3
description This study was supported by the Ministry of Science and Innovation (MICINN) and the Ministry of Economy and Competitiveness (MINECO) of the Spanish Government (Projects: MAT2009-12547, MAT2012-30706 co-funded by the European Union through European Regional Development Funds). C.M.-M. thanks the support of the Secretary for Universities and Research of the Ministry of Economy and Knowledge of the Government of Catalonia (2011-BP-B-00042) and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7-PEOPLE-2012-CIG, REA grant agreement n° 321985).
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/2946
http://dx.doi.org/10.1021/bm501528x
url http://hdl.handle.net/20.500.12328/2946
http://dx.doi.org/10.1021/bm501528x
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biomacromolecules
2;
info:eu-repo/grantAgreement/ES/MICINN/MAT2009-12547
info:eu-repo/grantAgreement/ES/MINECO/MAT2012-30706
info:eu-repo/grantAgreement/EC/FP7/321985
dc.rights.none.fl_str_mv © 2014 American Chemical Society
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2014 American Chemical Society
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411954738593792
score 15,812429

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