Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport

Bioinspired free-standing nanomembranes (FSNMs) for selective ion transport have been tailored by immobilizing the Omp2a ß-barrel membrane protein inside nanoperforations created in flexible poly(lactic acid) (PLA) nanomembranes. Perforated PLA FSNMs have been prepared by spin-coating a 99 : 1 PLA :...

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Autores: Puiggalí Jou, Anna|||0000-0002-2234-9436, Pérez Madrigal, Maria del Mar|||0000-0002-2498-8485, Valle Mendoza, Luis Javier del|||0000-0001-9916-1741, Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696, Casas Becerra, María Teresa|||0000-0002-5309-8246, Michaux, Catherine, Perpete, Eric, Estrany Coda, Francesc|||0000-0002-2696-1489, Alemán Llansó, Carlos|||0000-0003-4462-6075
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/91323
Acceso en línea:https://hdl.handle.net/2117/91323
https://dx.doi.org/10.1039/c6nr04948f
Access Level:acceso abierto
Palabra clave:Nanostructured materials
Biopolymers
Biopolímers
Materials nanoestructurals
Electrofilatura
Àrees temàtiques de la UPC::Enginyeria química
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spelling Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transportPuiggalí Jou, Anna|||0000-0002-2234-9436Pérez Madrigal, Maria del Mar|||0000-0002-2498-8485Valle Mendoza, Luis Javier del|||0000-0001-9916-1741Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696Casas Becerra, María Teresa|||0000-0002-5309-8246Michaux, CatherinePerpete, EricEstrany Coda, Francesc|||0000-0002-2696-1489Alemán Llansó, Carlos|||0000-0003-4462-6075Nanostructured materialsBiopolymersBiopolímersMaterials nanoestructuralsElectrofilaturaÀrees temàtiques de la UPC::Enginyeria químicaBioinspired free-standing nanomembranes (FSNMs) for selective ion transport have been tailored by immobilizing the Omp2a ß-barrel membrane protein inside nanoperforations created in flexible poly(lactic acid) (PLA) nanomembranes. Perforated PLA FSNMs have been prepared by spin-coating a 99 : 1 PLA : poly(vinyl alcohol) mixture, and through a phase segregation process nanofeatures with dimensions similar to the entire nanomembrane thickness (~110 nm) were induced. These nanofeatures have subsequently been transformed into nanoperforations (diameter: ~51 nm) by selective solvent etching. The protein confined inside the nanopores of PLA FSNMs preserves the ß-barrel structure and organizes in ovoid aggregates. The transport properties of Na+, K+, and Ca2+ across non-perforated PLA, nanoperforated PLA, and Omp2a-filled nanoperforated PLA have been monitored by measuring the nanomembrane resistance with electrochemical impedance spectroscopy (EIS). The incorporation of nanoperforations enhances the transport of ions across PLA nanomembranes, whereas the functionality of immobilized Omp2a is essential to exhibit effects similar to those observed in biological nanomembranes. Indeed, Omp2a-filled nanoperforated PLA nanomembranes exhibit stronger affinity towards Na+ and Ca2+ ions than towards K+. In summary, this work provides a novel bioinspired strategy to develop mechanically stable and flexible FSNMs with channels for ion transport, which are precisely located inside artificial nanoperforations, thus holding great potential for applications in biofiltration and biosensingPeer Reviewed20162016-10-1420162016-11-02journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/91323https://dx.doi.org/10.1039/c6nr04948freponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/913232026-05-27T15:37:01Z
dc.title.none.fl_str_mv Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
title Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
spellingShingle Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
Puiggalí Jou, Anna|||0000-0002-2234-9436
Nanostructured materials
Biopolymers
Biopolímers
Materials nanoestructurals
Electrofilatura
Àrees temàtiques de la UPC::Enginyeria química
title_short Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
title_full Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
title_fullStr Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
title_full_unstemmed Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
title_sort Confinement of a ß-barrel protein in nanoperforated free-standing nanomembranes for ion transport
dc.creator.none.fl_str_mv Puiggalí Jou, Anna|||0000-0002-2234-9436
Pérez Madrigal, Maria del Mar|||0000-0002-2498-8485
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Casas Becerra, María Teresa|||0000-0002-5309-8246
Michaux, Catherine
Perpete, Eric
Estrany Coda, Francesc|||0000-0002-2696-1489
Alemán Llansó, Carlos|||0000-0003-4462-6075
author Puiggalí Jou, Anna|||0000-0002-2234-9436
author_facet Puiggalí Jou, Anna|||0000-0002-2234-9436
Pérez Madrigal, Maria del Mar|||0000-0002-2498-8485
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Casas Becerra, María Teresa|||0000-0002-5309-8246
Michaux, Catherine
Perpete, Eric
Estrany Coda, Francesc|||0000-0002-2696-1489
Alemán Llansó, Carlos|||0000-0003-4462-6075
author_role author
author2 Pérez Madrigal, Maria del Mar|||0000-0002-2498-8485
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696
Casas Becerra, María Teresa|||0000-0002-5309-8246
Michaux, Catherine
Perpete, Eric
Estrany Coda, Francesc|||0000-0002-2696-1489
Alemán Llansó, Carlos|||0000-0003-4462-6075
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanostructured materials
Biopolymers
Biopolímers
Materials nanoestructurals
Electrofilatura
Àrees temàtiques de la UPC::Enginyeria química
topic Nanostructured materials
Biopolymers
Biopolímers
Materials nanoestructurals
Electrofilatura
Àrees temàtiques de la UPC::Enginyeria química
description Bioinspired free-standing nanomembranes (FSNMs) for selective ion transport have been tailored by immobilizing the Omp2a ß-barrel membrane protein inside nanoperforations created in flexible poly(lactic acid) (PLA) nanomembranes. Perforated PLA FSNMs have been prepared by spin-coating a 99 : 1 PLA : poly(vinyl alcohol) mixture, and through a phase segregation process nanofeatures with dimensions similar to the entire nanomembrane thickness (~110 nm) were induced. These nanofeatures have subsequently been transformed into nanoperforations (diameter: ~51 nm) by selective solvent etching. The protein confined inside the nanopores of PLA FSNMs preserves the ß-barrel structure and organizes in ovoid aggregates. The transport properties of Na+, K+, and Ca2+ across non-perforated PLA, nanoperforated PLA, and Omp2a-filled nanoperforated PLA have been monitored by measuring the nanomembrane resistance with electrochemical impedance spectroscopy (EIS). The incorporation of nanoperforations enhances the transport of ions across PLA nanomembranes, whereas the functionality of immobilized Omp2a is essential to exhibit effects similar to those observed in biological nanomembranes. Indeed, Omp2a-filled nanoperforated PLA nanomembranes exhibit stronger affinity towards Na+ and Ca2+ ions than towards K+. In summary, this work provides a novel bioinspired strategy to develop mechanically stable and flexible FSNMs with channels for ion transport, which are precisely located inside artificial nanoperforations, thus holding great potential for applications in biofiltration and biosensing
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-10-14
2016
2016-11-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/91323
https://dx.doi.org/10.1039/c6nr04948f
url https://hdl.handle.net/2117/91323
https://dx.doi.org/10.1039/c6nr04948f
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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