Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate

Staphylococcus aureus is regarded as a threatening bacterial pathogen causing invasive pneumonia in healthcare settings and in the community. The continuous emergence of multidrug resistant strains is narrowing the treatment options for these infections. The development of an effective S. aureus vac...

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Detalles Bibliográficos
Autores: García Fernández, Patricia, Póvoa Cabral, María Clara, Beceiro Casas, Alejandro, Moscoso Naya, Miriam, Bou Arévalo, Germán
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/21294
Acceso en línea:https://portalcientifico.sergas.gal//documentos/6416a5095db420433b7b62f3
http://hdl.handle.net/20.500.11940/21294
Access Level:acceso abierto
Palabra clave:AS A Coruña
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spelling Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine CandidateGarcía Fernández, PatriciaPóvoa Cabral, María ClaraBeceiro Casas, AlejandroMoscoso Naya, MiriamBou Arévalo, GermánAS A CoruñaCHUACAS A CoruñaINIBICAS A CoruñaCHUACAS A CoruñaCHUACAS A CoruñaCHUACStaphylococcus aureus is regarded as a threatening bacterial pathogen causing invasive pneumonia in healthcare settings and in the community. The continuous emergence of multidrug resistant strains is narrowing the treatment options for these infections. The development of an effective S. aureus vaccine is, therefore, a global priority. We have previously developed a vaccine candidate, 132 ?murI ?dat, which is auxotrophic for D-glutamate, and protects against sepsis caused by S. aureus. In the present study, we explored the potential of this vaccine candidate to prevent staphylococcal pneumonia, by using an acute lung infection model in BALB/c mice. Intranasal inoculation of the vaccine strain yielded transitory colonization of the lung tissue, stimulated production of relevant serum IgG and secretory IgA antibodies in the lung and distal vaginal mucosa and conferred cross-protection to acute pneumonia caused by clinically important S. aureus strains. Although these findings are promising, additional research is needed to minimize dose-dependent toxicity for safer intranasal immunization with this vaccine candidate.This work was supported by a grant from the Servicio Galego de Saude (SERGAS)-Galician Healthcare Service (Programs Innova Saude), the Spanish Network for Research in Infectious Diseases (RD12/0015/0014 and RD16/0016/0006) and Centro de Investigacion Biomedica en Red de Enfermedades Infecciosas (CIBERINFEC). The work was funded by Instituto Carlos III (ISCIII) through projects PI15/00860, PI18/00501 and PI21/00704 to GB; and PI20/01212 to AB, co-funded by European Regional Development Fund A way to make Europe; and by GAIN-Agencia Gallega de Innovacion-Conselleria de Economia, Emprego e Industria through the project IN607D 2021/12 to AB. PG was supported by the Spanish Network for Research in Infectious Diseases (RD12/0015/0014 and RD16/0016/0006). MPC was supported by a PhD scholarship (SFRH/BD/64740/2009) from Portugal and POPH/FSE.2023info:eu-repo/semantics/articlehttps://portalcientifico.sergas.gal//documentos/6416a5095db420433b7b62f3http://hdl.handle.net/20.500.11940/21294reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/212942026-06-12T08:40:47Z
dc.title.none.fl_str_mv Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
title Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
spellingShingle Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
García Fernández, Patricia
AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
title_short Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
title_full Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
title_fullStr Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
title_full_unstemmed Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
title_sort Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate
dc.creator.none.fl_str_mv García Fernández, Patricia
Póvoa Cabral, María Clara
Beceiro Casas, Alejandro
Moscoso Naya, Miriam
Bou Arévalo, Germán
author García Fernández, Patricia
author_facet García Fernández, Patricia
Póvoa Cabral, María Clara
Beceiro Casas, Alejandro
Moscoso Naya, Miriam
Bou Arévalo, Germán
author_role author
author2 Póvoa Cabral, María Clara
Beceiro Casas, Alejandro
Moscoso Naya, Miriam
Bou Arévalo, Germán
author2_role author
author
author
author
dc.subject.none.fl_str_mv AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
topic AS A Coruña
CHUAC
AS A Coruña
INIBIC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
AS A Coruña
CHUAC
description Staphylococcus aureus is regarded as a threatening bacterial pathogen causing invasive pneumonia in healthcare settings and in the community. The continuous emergence of multidrug resistant strains is narrowing the treatment options for these infections. The development of an effective S. aureus vaccine is, therefore, a global priority. We have previously developed a vaccine candidate, 132 ?murI ?dat, which is auxotrophic for D-glutamate, and protects against sepsis caused by S. aureus. In the present study, we explored the potential of this vaccine candidate to prevent staphylococcal pneumonia, by using an acute lung infection model in BALB/c mice. Intranasal inoculation of the vaccine strain yielded transitory colonization of the lung tissue, stimulated production of relevant serum IgG and secretory IgA antibodies in the lung and distal vaginal mucosa and conferred cross-protection to acute pneumonia caused by clinically important S. aureus strains. Although these findings are promising, additional research is needed to minimize dose-dependent toxicity for safer intranasal immunization with this vaccine candidate.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://portalcientifico.sergas.gal//documentos/6416a5095db420433b7b62f3
http://hdl.handle.net/20.500.11940/21294
url https://portalcientifico.sergas.gal//documentos/6416a5095db420433b7b62f3
http://hdl.handle.net/20.500.11940/21294
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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