Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response

Producción Científica

Detalles Bibliográficos
Autores: Lorenzo Martín, Elvira, Gallego Muñoz, Patricia, Ibares Frías, Lucía, Marcos, Susana, Pérez Merino, Pablo, Fernández Martínez, Itziar, Kochevar, Irene E, Martínez García, María del Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/45095
Acceso en línea:https://doi.org/10.1167/iovs.18-24881
http://uvadoc.uva.es/handle/10324/45095
Access Level:acceso abierto
Palabra clave:Wound repair
Cicatrización
Cross-linking technique
Técnica de cross-linking
Rose bengal
Rosa de Bengala
Ultraviolet radiation
Radiación ultravioleta
Cornea
Córnea
2209.15 Optometría
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oai_identifier_str oai:uvadoc.uva.es:10324/45095
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spelling Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair responseLorenzo Martín, ElviraGallego Muñoz, PatriciaIbares Frías, LucíaMarcos, SusanaPérez Merino, PabloFernández Martínez, ItziarKochevar, Irene EMartínez García, María del CarmenWound repairCicatrizaciónCross-linking techniqueTécnica de cross-linkingRose bengalRosa de BengalaUltraviolet radiationRadiación ultravioletaCorneaCórnea2209.15 OptometríaProducción CientíficaPurpose: To study corneal wound healing after two cross-linking techniques using either rose bengal and green light (RGX) or the conventional treatment using riboflavin and UVA radiation (UVX). Methods: Corneas of New Zealand rabbits were monolaterally treated with UVX (21 eyes) or RGX (25 eyes). Treatments involved corneal de-epithelialization (8-mm diameter), soaking with photosensitizer (0.1% riboflavin in 20% dextran for 30 minutes for UVX; 0.1% rose bengal for 2 minutes for RGX), and light irradiation (370 nm, 3 mW/cm2, 30 minutes for UVX; 532 nm, 0.25 W/cm2, 7 minutes for RGX). Contralateral eyes were used as controls. Clinical follow-up included fluorescein staining, haze measurement, and pachymetry. Healing events analyzed after euthanasia at 2, 30, and 60 days included cell death (TUNEL assay), cell proliferation (BrdU [bromodeoxyuridine] immunofluorescence), and differentiation to myofibroblasts (α-SMA [alpha smooth muscle actin] immunohistochemistry). Results: Re-epithelialization and pachymetries were similar after RGX and UVX. The haze from day 1 to 15 was greater after UVX. Cell death was deeper after UVX, being localized in the anterior and middle stroma, and was superficial (anterior third) after RGX. Cell proliferation appeared after 2 days and was localized in the middle and posterior stroma in the UVX group but was superficial in the RGX group. After 60 days the number of stromal cells had not returned to the control number in either group. Conclusions: The deeper and longer-lasting cell damage caused by UVX compared to RGX may underlie the slower cell repopulation after UVX and other differences in healing. Shallower damage and a shorter treatment time suggest that RGX may be appropriate for stiffening thin corneas.Consejo Europeo de Investigación (grant ERC-2011-AdC-294099)Gobierno de España (grants FIS2011-25637-R, FIS2014- 56643-R and FIS2017-84753-R)Association for Research in Vision and Ophthalmology2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1167/iovs.18-24881http://uvadoc.uva.es/handle/10324/45095reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://iovs.arvojournals.org/article.aspx?articleid=2705917info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/450952026-06-13T12:44:47Z
dc.title.none.fl_str_mv Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
spellingShingle Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
Lorenzo Martín, Elvira
Wound repair
Cicatrización
Cross-linking technique
Técnica de cross-linking
Rose bengal
Rosa de Bengala
Ultraviolet radiation
Radiación ultravioleta
Cornea
Córnea
2209.15 Optometría
title_short Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_full Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_fullStr Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_full_unstemmed Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_sort Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
dc.creator.none.fl_str_mv Lorenzo Martín, Elvira
Gallego Muñoz, Patricia
Ibares Frías, Lucía
Marcos, Susana
Pérez Merino, Pablo
Fernández Martínez, Itziar
Kochevar, Irene E
Martínez García, María del Carmen
author Lorenzo Martín, Elvira
author_facet Lorenzo Martín, Elvira
Gallego Muñoz, Patricia
Ibares Frías, Lucía
Marcos, Susana
Pérez Merino, Pablo
Fernández Martínez, Itziar
Kochevar, Irene E
Martínez García, María del Carmen
author_role author
author2 Gallego Muñoz, Patricia
Ibares Frías, Lucía
Marcos, Susana
Pérez Merino, Pablo
Fernández Martínez, Itziar
Kochevar, Irene E
Martínez García, María del Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wound repair
Cicatrización
Cross-linking technique
Técnica de cross-linking
Rose bengal
Rosa de Bengala
Ultraviolet radiation
Radiación ultravioleta
Cornea
Córnea
2209.15 Optometría
topic Wound repair
Cicatrización
Cross-linking technique
Técnica de cross-linking
Rose bengal
Rosa de Bengala
Ultraviolet radiation
Radiación ultravioleta
Cornea
Córnea
2209.15 Optometría
description Producción Científica
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1167/iovs.18-24881
http://uvadoc.uva.es/handle/10324/45095
url https://doi.org/10.1167/iovs.18-24881
http://uvadoc.uva.es/handle/10324/45095
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://iovs.arvojournals.org/article.aspx?articleid=2705917
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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