Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota

Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to scr...

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Autores: Ruas-Madiedo, Patricia, Moreno Muñoz, José Antonio, Salazar, Nuria, Delgado, Susana, Mayo Pérez, Baltasar, Margolles Barros, Abelardo, González de los Reyes-Gavilán, Clara
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/7074
Acceso en línea:http://hdl.handle.net/10261/7074
Access Level:acceso abierto
Palabra clave:Exopolisacáridos
Lactobacilos
Bifidobacterias
Microbiótica intestinal
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spelling Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal MicrobiotaRuas-Madiedo, PatriciaMoreno Muñoz, José AntonioSalazar, NuriaDelgado, SusanaMayo Pérez, BaltasarMargolles Barros, AbelardoGonzález de los Reyes-Gavilán, ClaraExopolisacáridosLactobacilosBifidobacteriasMicrobiótica intestinalUsing phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strainsThis work was financed by FEDER funds (European Union) and the Spanish Plan Nacional de I+D+I through project AGL 2004-06088-CO2-01/ALI and by a grant from the Fundación Príncipe de Asturias (Beca Grande Covián 2003). P. Ruas-Madiedo and J. A. Moreno were the recipients of an I3P postdoctoral research contract granted by CSIC. S. Delgado and N. Salazar acknowledge the Spanish Ministry of Education and Science for their respective fellowships (Programa FPI).Peer reviewedAmerican Society for MicrobiologyMinisterio de Educación y Ciencia (España)Fundación Príncipe de AsturiasComisión Interministerial de Ciencia y Tecnología, CICYT (España)European Commission200820082007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65011288226 bytesapplication/pdfhttp://hdl.handle.net/10261/7074reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/70742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
title Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
spellingShingle Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
Ruas-Madiedo, Patricia
Exopolisacáridos
Lactobacilos
Bifidobacterias
Microbiótica intestinal
title_short Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
title_full Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
title_fullStr Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
title_full_unstemmed Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
title_sort Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota
dc.creator.none.fl_str_mv Ruas-Madiedo, Patricia
Moreno Muñoz, José Antonio
Salazar, Nuria
Delgado, Susana
Mayo Pérez, Baltasar
Margolles Barros, Abelardo
González de los Reyes-Gavilán, Clara
author Ruas-Madiedo, Patricia
author_facet Ruas-Madiedo, Patricia
Moreno Muñoz, José Antonio
Salazar, Nuria
Delgado, Susana
Mayo Pérez, Baltasar
Margolles Barros, Abelardo
González de los Reyes-Gavilán, Clara
author_role author
author2 Moreno Muñoz, José Antonio
Salazar, Nuria
Delgado, Susana
Mayo Pérez, Baltasar
Margolles Barros, Abelardo
González de los Reyes-Gavilán, Clara
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Fundación Príncipe de Asturias
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
European Commission
dc.subject.none.fl_str_mv Exopolisacáridos
Lactobacilos
Bifidobacterias
Microbiótica intestinal
topic Exopolisacáridos
Lactobacilos
Bifidobacterias
Microbiótica intestinal
description Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains
publishDate 2007
dc.date.none.fl_str_mv 2007
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/7074
url http://hdl.handle.net/10261/7074
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1288226 bytes
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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