Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)

The interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar...

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Autores: Delmani, Fatima Azzahra, Torreblanca López, José, Moreno Onorato, Francisco Javier, García Herdugo, Gregorio, Vilaplana Serrano, Rosario, González Vílchez, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/36721
Acceso en línea:http://hdl.handle.net/11441/36721
Access Level:acceso abierto
Palabra clave:Antitumor drugs
DNA-damage
Fibrillarin
RAP
Tumor cells in vitro
UBF
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spelling Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)Delmani, Fatima AzzahraTorreblanca López, JoséMoreno Onorato, Francisco JavierGarcía Herdugo, GregorioVilaplana Serrano, RosarioGonzález Vílchez, FranciscoAntitumor drugsDNA-damageFibrillarinRAPTumor cells in vitroUBFThe interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and fibrillarin) involved in DNA transcription, rRNA processing, and ribosomal assembly. The results showed that RAP binds to the DNA of two cell lines (H4 and Hs-683) causing a delay in cell proliferation rate leading to a number of cellular modifications. These modifications include DNA-damage assessed by the single cell gel electrophoresis method (comet assay) and variation in the expression of nucleolar proteins; UBF was more abundant in RAP treated cells, this was explained by the high affinity of this protein to DNA modified by RAP. On the other hand, fibrillarin was found in less quantities in RAP treated cells which was explained by a de-regulation of the ribosomal machinery caused by RAPNational Institute of Science Communication and Information ResourcesBiología CelularQuímica Inorgánica2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/36721reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésIndian Journal of Experimental Biology (IJEB), 52(6), 579-588http://nopr.niscair.res.in/bitstream/123456789/28864/1/IJEB%2052%286%29%20579-588.pdfinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/367212026-06-17T12:51:07Z
dc.title.none.fl_str_mv Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
title Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
spellingShingle Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
Delmani, Fatima Azzahra
Antitumor drugs
DNA-damage
Fibrillarin
RAP
Tumor cells in vitro
UBF
title_short Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
title_full Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
title_fullStr Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
title_full_unstemmed Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
title_sort Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP)
dc.creator.none.fl_str_mv Delmani, Fatima Azzahra
Torreblanca López, José
Moreno Onorato, Francisco Javier
García Herdugo, Gregorio
Vilaplana Serrano, Rosario
González Vílchez, Francisco
author Delmani, Fatima Azzahra
author_facet Delmani, Fatima Azzahra
Torreblanca López, José
Moreno Onorato, Francisco Javier
García Herdugo, Gregorio
Vilaplana Serrano, Rosario
González Vílchez, Francisco
author_role author
author2 Torreblanca López, José
Moreno Onorato, Francisco Javier
García Herdugo, Gregorio
Vilaplana Serrano, Rosario
González Vílchez, Francisco
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular
Química Inorgánica
dc.subject.none.fl_str_mv Antitumor drugs
DNA-damage
Fibrillarin
RAP
Tumor cells in vitro
UBF
topic Antitumor drugs
DNA-damage
Fibrillarin
RAP
Tumor cells in vitro
UBF
description The interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and fibrillarin) involved in DNA transcription, rRNA processing, and ribosomal assembly. The results showed that RAP binds to the DNA of two cell lines (H4 and Hs-683) causing a delay in cell proliferation rate leading to a number of cellular modifications. These modifications include DNA-damage assessed by the single cell gel electrophoresis method (comet assay) and variation in the expression of nucleolar proteins; UBF was more abundant in RAP treated cells, this was explained by the high affinity of this protein to DNA modified by RAP. On the other hand, fibrillarin was found in less quantities in RAP treated cells which was explained by a de-regulation of the ribosomal machinery caused by RAP
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/36721
url http://hdl.handle.net/11441/36721
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Indian Journal of Experimental Biology (IJEB), 52(6), 579-588
http://nopr.niscair.res.in/bitstream/123456789/28864/1/IJEB%2052%286%29%20579-588.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Institute of Science Communication and Information Resources
publisher.none.fl_str_mv National Institute of Science Communication and Information Resources
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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