Micropropagation of mature Quercus ilex L. trees by axillary budding

This paper reports the successful micropropagation of mature Quercus ilex trees known as reluctant to in vitro propagation. Crown branch segments collected from 30 and100 year-old trees were forced in order to promote the production of sprouting shoots that were used as a source of explants for init...

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Autores: Martínez-Santiago, María Teresa, Corredoira, Elena, Viéitez Martín, Ana María, Cernadas, María José, Montenegro, R., Ballester, Antonio, Viéitez Madriñán, Francisco Javier, San José, M. Carmen
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::2129804efbb1d4733cf91fe62907f918
Acceso en línea:http://hdl.handle.net/10261/178278
Access Level:acceso abierto
Palabra clave:Holm oak
Ethylene inhibitors
Gelling agents
Rejuvenation
Rooting
Shoot culture initiation
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spelling Micropropagation of mature Quercus ilex L. trees by axillary buddingMartínez-Santiago, María TeresaCorredoira, ElenaViéitez Martín, Ana MaríaCernadas, María JoséMontenegro, R.Ballester, AntonioViéitez Madriñán, Francisco JavierSan José, M. CarmenHolm oakEthylene inhibitorsGelling agentsRejuvenationRootingShoot culture initiationThis paper reports the successful micropropagation of mature Quercus ilex trees known as reluctant to in vitro propagation. Crown branch segments collected from 30 and100 year-old trees were forced in order to promote the production of sprouting shoots that were used as a source of explants for initiating the cultures. Sterilization was critical and required low-level disinfestation protocols. Six out of the eight mature genotypes attempted were successfully inoculated and then maintained in culture with varying responses. Shoot proliferation of holm oak was influenced by BA concentration, with improved multiplication and shoot appearance when the BA concentration was sequentially reduced over the culture period. Micropropagation by axillary budding was achieved by culturing shoots on a sequence of cytokinin-enriched Lloyd and McCown (WPM) media alternating 2 week-long subcultures on 0.44 μM benzyadenine (BA) first, followed by 0.22 μM BA, then 0.044μM BA plus 0.46μM zeatin. Sucrose concentration and agar brand affected shoot proliferation, and the best results were obtained on WPM medium supplemented with 8 g L-1 Sigma agar (A-1296; Sigma-Aldrich) and 30 g L-1 sucrose. Addition of 20 μM silver thiosulphate had a significant positive effect on the appearance and development of shoots with a higher number of shoots being healthy and showing reduced shoot tip necrosis and early senescence of leaves. The 18.8% of the microshoots obtained for one clone could be rooted within 15 days on a half-strength Murashige and Skoog medium containing 14.8μM or 24.6μM indole-3-butyric acid and 0.54 μM -naphthalene acetic acid.Peer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/178278reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::2129804efbb1d4733cf91fe62907f9182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Micropropagation of mature Quercus ilex L. trees by axillary budding
title Micropropagation of mature Quercus ilex L. trees by axillary budding
spellingShingle Micropropagation of mature Quercus ilex L. trees by axillary budding
Martínez-Santiago, María Teresa
Holm oak
Ethylene inhibitors
Gelling agents
Rejuvenation
Rooting
Shoot culture initiation
title_short Micropropagation of mature Quercus ilex L. trees by axillary budding
title_full Micropropagation of mature Quercus ilex L. trees by axillary budding
title_fullStr Micropropagation of mature Quercus ilex L. trees by axillary budding
title_full_unstemmed Micropropagation of mature Quercus ilex L. trees by axillary budding
title_sort Micropropagation of mature Quercus ilex L. trees by axillary budding
dc.creator.none.fl_str_mv Martínez-Santiago, María Teresa
Corredoira, Elena
Viéitez Martín, Ana María
Cernadas, María José
Montenegro, R.
Ballester, Antonio
Viéitez Madriñán, Francisco Javier
San José, M. Carmen
author Martínez-Santiago, María Teresa
author_facet Martínez-Santiago, María Teresa
Corredoira, Elena
Viéitez Martín, Ana María
Cernadas, María José
Montenegro, R.
Ballester, Antonio
Viéitez Madriñán, Francisco Javier
San José, M. Carmen
author_role author
author2 Corredoira, Elena
Viéitez Martín, Ana María
Cernadas, María José
Montenegro, R.
Ballester, Antonio
Viéitez Madriñán, Francisco Javier
San José, M. Carmen
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Holm oak
Ethylene inhibitors
Gelling agents
Rejuvenation
Rooting
Shoot culture initiation
topic Holm oak
Ethylene inhibitors
Gelling agents
Rejuvenation
Rooting
Shoot culture initiation
description This paper reports the successful micropropagation of mature Quercus ilex trees known as reluctant to in vitro propagation. Crown branch segments collected from 30 and100 year-old trees were forced in order to promote the production of sprouting shoots that were used as a source of explants for initiating the cultures. Sterilization was critical and required low-level disinfestation protocols. Six out of the eight mature genotypes attempted were successfully inoculated and then maintained in culture with varying responses. Shoot proliferation of holm oak was influenced by BA concentration, with improved multiplication and shoot appearance when the BA concentration was sequentially reduced over the culture period. Micropropagation by axillary budding was achieved by culturing shoots on a sequence of cytokinin-enriched Lloyd and McCown (WPM) media alternating 2 week-long subcultures on 0.44 μM benzyadenine (BA) first, followed by 0.22 μM BA, then 0.044μM BA plus 0.46μM zeatin. Sucrose concentration and agar brand affected shoot proliferation, and the best results were obtained on WPM medium supplemented with 8 g L-1 Sigma agar (A-1296; Sigma-Aldrich) and 30 g L-1 sucrose. Addition of 20 μM silver thiosulphate had a significant positive effect on the appearance and development of shoots with a higher number of shoots being healthy and showing reduced shoot tip necrosis and early senescence of leaves. The 18.8% of the microshoots obtained for one clone could be rooted within 15 days on a half-strength Murashige and Skoog medium containing 14.8μM or 24.6μM indole-3-butyric acid and 0.54 μM -naphthalene acetic acid.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/178278
url http://hdl.handle.net/10261/178278
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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