Active films based on cocoa extract with antioxidant, antimicrobial and biological applications

Novel films of ethylene-vinyl alcohol copolymer (EVOH) containing flavonoid-rich cocoa were developed. To understand their potential application as active packaging material, antioxidant and antimicrobial properties of the films were determined as well as the antioxidant activity of the release comp...

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Detalles Bibliográficos
Autores: Arroyo Calatayud, Marta, López de Dicastillo, Carol, López-Carballo, Gracia, Vélez, Dinoraz, Hernández-Muñoz, Pilar, Gavara, Rafael
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376868
Acceso en línea:http://hdl.handle.net/10261/376868
https://api.elsevier.com/content/abstract/scopus_id/84875959842
Access Level:acceso abierto
Palabra clave:Active packaging
Antimicrobial film
Antioxidant release
Caco-2 cells
Cocoa
EVOH
active packaging
antioxidants
antimicrobial properties
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spelling Active films based on cocoa extract with antioxidant, antimicrobial and biological applicationsArroyo Calatayud, MartaLópez de Dicastillo, CarolLópez-Carballo, GraciaVélez, DinorazHernández-Muñoz, PilarGavara, RafaelActive packagingAntimicrobial filmAntioxidant releaseCaco-2 cellsCocoaEVOHactive packagingantioxidantsantimicrobial propertiesNovel films of ethylene-vinyl alcohol copolymer (EVOH) containing flavonoid-rich cocoa were developed. To understand their potential application as active packaging material, antioxidant and antimicrobial properties of the films were determined as well as the antioxidant activity of the release compounds in Caco-2 human epithelial colorectal adenocarcinoma cells. Exposure of the films to aqueous food simulant showed antioxidant capacity. The release of cocoa extract components was dependent on the antioxidant concentration incorporated in the film and on temperature. Cocoa extract and the fraction obtained after in vitro gastrointestinal digestion presented antioxidant activity against oxidative stress induced by hydrogen peroxide in Caco-2 cells. Films with 10%, 15%, and 20% cocoa extract produced bactericidal effect against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella enterica. The application of films to an infant milk formula, previously inoculated with L. monocytogenes, inhibited the growth of bacteria 1.5 log units the first day and showed sustained release, inhibiting 0.52 and 0.76 log units, respectively, by the sixth day, while cocoa powder added directly did not produce any effect.The authors acknowledge the financial support of the Spanish Ministry of Science and Innovation, Projects AGL2006-02176, AGL2009-08776 and Fun-C-Food CSD2007-00063, and the C. L-d-D fellowship (FPU program)Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/376868https://api.elsevier.com/content/abstract/scopus_id/84875959842reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MEC//AGL2006-02176info:eu-repo/grantAgreement/MICINN//AGL2009-08776Food chemistryhttps://doi.org/10.1016/j.foodchem.2013.01.097Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3768682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
title Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
spellingShingle Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
Arroyo Calatayud, Marta
Active packaging
Antimicrobial film
Antioxidant release
Caco-2 cells
Cocoa
EVOH
active packaging
antioxidants
antimicrobial properties
title_short Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
title_full Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
title_fullStr Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
title_full_unstemmed Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
title_sort Active films based on cocoa extract with antioxidant, antimicrobial and biological applications
dc.creator.none.fl_str_mv Arroyo Calatayud, Marta
López de Dicastillo, Carol
López-Carballo, Gracia
Vélez, Dinoraz
Hernández-Muñoz, Pilar
Gavara, Rafael
author Arroyo Calatayud, Marta
author_facet Arroyo Calatayud, Marta
López de Dicastillo, Carol
López-Carballo, Gracia
Vélez, Dinoraz
Hernández-Muñoz, Pilar
Gavara, Rafael
author_role author
author2 López de Dicastillo, Carol
López-Carballo, Gracia
Vélez, Dinoraz
Hernández-Muñoz, Pilar
Gavara, Rafael
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Active packaging
Antimicrobial film
Antioxidant release
Caco-2 cells
Cocoa
EVOH
active packaging
antioxidants
antimicrobial properties
topic Active packaging
Antimicrobial film
Antioxidant release
Caco-2 cells
Cocoa
EVOH
active packaging
antioxidants
antimicrobial properties
description Novel films of ethylene-vinyl alcohol copolymer (EVOH) containing flavonoid-rich cocoa were developed. To understand their potential application as active packaging material, antioxidant and antimicrobial properties of the films were determined as well as the antioxidant activity of the release compounds in Caco-2 human epithelial colorectal adenocarcinoma cells. Exposure of the films to aqueous food simulant showed antioxidant capacity. The release of cocoa extract components was dependent on the antioxidant concentration incorporated in the film and on temperature. Cocoa extract and the fraction obtained after in vitro gastrointestinal digestion presented antioxidant activity against oxidative stress induced by hydrogen peroxide in Caco-2 cells. Films with 10%, 15%, and 20% cocoa extract produced bactericidal effect against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella enterica. The application of films to an infant milk formula, previously inoculated with L. monocytogenes, inhibited the growth of bacteria 1.5 log units the first day and showed sustained release, inhibiting 0.52 and 0.76 log units, respectively, by the sixth day, while cocoa powder added directly did not produce any effect.
publishDate 2013
dc.date.none.fl_str_mv 2013
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376868
https://api.elsevier.com/content/abstract/scopus_id/84875959842
url http://hdl.handle.net/10261/376868
https://api.elsevier.com/content/abstract/scopus_id/84875959842
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MEC//AGL2006-02176
info:eu-repo/grantAgreement/MICINN//AGL2009-08776
Food chemistry
https://doi.org/10.1016/j.foodchem.2013.01.097
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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