Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping

Abstract Background Cucumber, Cucumis sativus L. (2n = 2 × = 14) and melon, C. melo L. (2n = 2 × = 24) are two important vegetable species in the genus Cucumis (family Cucurbitaceae). Both species have an Asian origin that diverged approximately nine million years ago. Cucumber is believed to have e...

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Autores: Li, Dawei, Cuevas, Hugo E., Yang, Luming, Li, Yuhong, García-Mas, Jordi, Zalapa, Juan, Staub, Jack E., Luan, Feishi, Reddy, Umesh K., He, Xiaoming, Gong, Zhenhui, Weng, Yiqun
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/39117
Acceso en línea:http://dx.doi.org/10.1186/1471-2164-12-396
http://hdl.handle.net/10261/39117
Access Level:acceso abierto
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spelling Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic MappingLi, DaweiCuevas, Hugo E.Yang, LumingLi, YuhongGarcía-Mas, JordiZalapa, JuanStaub, Jack E.Luan, FeishiReddy, Umesh K.He, XiaomingGong, ZhenhuiWeng, YiqunAbstract Background Cucumber, Cucumis sativus L. (2n = 2 × = 14) and melon, C. melo L. (2n = 2 × = 24) are two important vegetable species in the genus Cucumis (family Cucurbitaceae). Both species have an Asian origin that diverged approximately nine million years ago. Cucumber is believed to have evolved from melon through chromosome fusion, but the details of this process are largely unknown. In this study, comparative genetic mapping between cucumber and melon was conducted to examine syntenic relationships of their chromosomes. Results Using two melon mapping populations, 154 and 127 cucumber SSR markers were added onto previously reported F2- and RIL-based genetic maps, respectively. A consensus melon linkage map was developed through map integration, which contained 401 co-dominant markers in 12 linkage groups including 199 markers derived from the cucumber genome. Syntenic relationships between melon and cucumber chromosomes were inferred based on associations between markers on the consensus melon map and cucumber draft genome scaffolds. It was determined that cucumber Chromosome 7 was syntenic to melon Chromosome I. Cucumber Chromosomes 2 and 6 each contained genomic regions that were syntenic with melon chromosomes III+V+XI and III+VIII+XI, respectively. Likewise, cucumber Chromosomes 1, 3, 4, and 5 each was syntenic with genomic regions of two melon chromosomes previously designated as II+XII, IV+VI, VII+VIII, and IX+X, respectively. However, the marker orders in several syntenic blocks on these consensus linkage maps were not co-linear suggesting that more complicated structural changes beyond simple chromosome fusion events have occurred during the evolution of cucumber. Conclusions Comparative mapping conducted herein supported the hypothesis that cucumber chromosomes may be the result of chromosome fusion from a 24-chromosome progenitor species. Except for a possible inversion, cucumber Chromosome 7 has largely remained intact in the past nine million years since its divergence from melon. Meanwhile, many structural changes may have occurred during the evolution of the remaining six cucumber chromosomes. Further characterization of the genomic nature of Cucumis species closely related to cucumber and melon might provide a better understanding of the evolutionary history leading to modern cucumber.This research was supported by U.S. Department of Agriculture-Agriculture Research Service Current Research Information System (CRIS) project no. 3655-21000-037-00D. Feishi Luan’s work was partially supported by the ‘948 Project’ of the Agriculture Ministry of China (2010-S22).Peer ReviewedBioMed Central2011201120112011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://dx.doi.org/10.1186/1471-2164-12-396http://hdl.handle.net/10261/39117reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/391172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
title Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
spellingShingle Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
Li, Dawei
title_short Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
title_full Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
title_fullStr Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
title_full_unstemmed Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
title_sort Syntenic Relationships between Cucumber (Cucumis sativus L.) and Melon (C. melo L.) Chromosomes as Revealed by Comparative Genetic Mapping
dc.creator.none.fl_str_mv Li, Dawei
Cuevas, Hugo E.
Yang, Luming
Li, Yuhong
García-Mas, Jordi
Zalapa, Juan
Staub, Jack E.
Luan, Feishi
Reddy, Umesh K.
He, Xiaoming
Gong, Zhenhui
Weng, Yiqun
author Li, Dawei
author_facet Li, Dawei
Cuevas, Hugo E.
Yang, Luming
Li, Yuhong
García-Mas, Jordi
Zalapa, Juan
Staub, Jack E.
Luan, Feishi
Reddy, Umesh K.
He, Xiaoming
Gong, Zhenhui
Weng, Yiqun
author_role author
author2 Cuevas, Hugo E.
Yang, Luming
Li, Yuhong
García-Mas, Jordi
Zalapa, Juan
Staub, Jack E.
Luan, Feishi
Reddy, Umesh K.
He, Xiaoming
Gong, Zhenhui
Weng, Yiqun
author2_role author
author
author
author
author
author
author
author
author
author
author
description Abstract Background Cucumber, Cucumis sativus L. (2n = 2 × = 14) and melon, C. melo L. (2n = 2 × = 24) are two important vegetable species in the genus Cucumis (family Cucurbitaceae). Both species have an Asian origin that diverged approximately nine million years ago. Cucumber is believed to have evolved from melon through chromosome fusion, but the details of this process are largely unknown. In this study, comparative genetic mapping between cucumber and melon was conducted to examine syntenic relationships of their chromosomes. Results Using two melon mapping populations, 154 and 127 cucumber SSR markers were added onto previously reported F2- and RIL-based genetic maps, respectively. A consensus melon linkage map was developed through map integration, which contained 401 co-dominant markers in 12 linkage groups including 199 markers derived from the cucumber genome. Syntenic relationships between melon and cucumber chromosomes were inferred based on associations between markers on the consensus melon map and cucumber draft genome scaffolds. It was determined that cucumber Chromosome 7 was syntenic to melon Chromosome I. Cucumber Chromosomes 2 and 6 each contained genomic regions that were syntenic with melon chromosomes III+V+XI and III+VIII+XI, respectively. Likewise, cucumber Chromosomes 1, 3, 4, and 5 each was syntenic with genomic regions of two melon chromosomes previously designated as II+XII, IV+VI, VII+VIII, and IX+X, respectively. However, the marker orders in several syntenic blocks on these consensus linkage maps were not co-linear suggesting that more complicated structural changes beyond simple chromosome fusion events have occurred during the evolution of cucumber. Conclusions Comparative mapping conducted herein supported the hypothesis that cucumber chromosomes may be the result of chromosome fusion from a 24-chromosome progenitor species. Except for a possible inversion, cucumber Chromosome 7 has largely remained intact in the past nine million years since its divergence from melon. Meanwhile, many structural changes may have occurred during the evolution of the remaining six cucumber chromosomes. Further characterization of the genomic nature of Cucumis species closely related to cucumber and melon might provide a better understanding of the evolutionary history leading to modern cucumber.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://dx.doi.org/10.1186/1471-2164-12-396
http://hdl.handle.net/10261/39117
url http://dx.doi.org/10.1186/1471-2164-12-396
http://hdl.handle.net/10261/39117
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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