Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection

BACKGROUND: In this study, we further investigated the association of two biomarkers, CCL18 and A1AT, with bladder cancer (BCa) and evaluated the influence of potentially confounding factors in an experimental model. METHODS: In a cohort of 308 subjects (102 with BCa), urinary concentrations of CCL1...

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Autores: Miyake, Makito, Ross, Shanti, Lawton, Adrienne, Chang, Myron, Dai, Yunfeng, Mengual Brichs, Lourdes, Alcaraz Asensio, Antonio, Giacoia, Eva Gomes, Goodison, Steve, Rosser, Charles J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/61144
Acceso en línea:https://hdl.handle.net/2445/61144
Access Level:acceso abierto
Palabra clave:Càncer de bufeta
Marcadors tumorals
Assaigs clínics
Orina
Bladder cancer
Tumor markers
Clinical trials
Urine
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spelling Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detectionMiyake, MakitoRoss, ShantiLawton, AdrienneChang, MyronDai, YunfengMengual Brichs, LourdesAlcaraz Asensio, AntonioGiacoia, Eva GomesGoodison, SteveRosser, Charles J.Càncer de bufetaMarcadors tumoralsAssaigs clínicsOrinaBladder cancerTumor markersClinical trialsUrineBACKGROUND: In this study, we further investigated the association of two biomarkers, CCL18 and A1AT, with bladder cancer (BCa) and evaluated the influence of potentially confounding factors in an experimental model. METHODS: In a cohort of 308 subjects (102 with BCa), urinary concentrations of CCL18 and A1AT were assessed by enzyme-linked immunosorbent assay (ELISA). In an experimental model, benign or cancerous cells, in addition to blood, were added to urines from healthy controls and analyzed by ELISA. Lastly, immunohistochemical staining for CCL18 and A1AT in human bladder tumors was performed. RESULTS: Median urinary protein concentrations of CCL18 (52.84 pg/ml vs. 11.13 pg/ml, p < 0.0001) and A1AT (606.4 ng/ml vs. 120.0 ng/ml, p < 0.0001) were significantly elevated in BCa subjects compared to controls. Furthermore, the addition of whole blood to pooled normal urine resulted in a significant increase in both CCL18 and A1AT. IHC staining of bladder tumors revealed CCL18 immunoreactivity in inflammatory cells only, and there was no significant increase in these immunoreactive cells within benign and cancerous tissue and no association with BCa grade nor stage was noted. A1AT immunoreactivity was observed in the cytoplasm of epithelia cells and intensity of immunostaining increased with tumor grade, but not tumor stage. CONCLUSIONS: Further development of A1AT as a diagnostic biomarker for BCa is warranted.BioMed Central2015201520132015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/61144Articles publicats en revistes (Ciències Fisiològiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1186/1471-2490-13-42BMC Urology , 2013, vol. 13, num. 1, p. 42http://dx.doi.org/10.1186/1471-2490-13-42cc-by (c) Miyake, M. et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/611442026-05-29T05:05:01Z
dc.title.none.fl_str_mv Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
title Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
spellingShingle Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
Miyake, Makito
Càncer de bufeta
Marcadors tumorals
Assaigs clínics
Orina
Bladder cancer
Tumor markers
Clinical trials
Urine
title_short Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
title_full Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
title_fullStr Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
title_full_unstemmed Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
title_sort Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection
dc.creator.none.fl_str_mv Miyake, Makito
Ross, Shanti
Lawton, Adrienne
Chang, Myron
Dai, Yunfeng
Mengual Brichs, Lourdes
Alcaraz Asensio, Antonio
Giacoia, Eva Gomes
Goodison, Steve
Rosser, Charles J.
author Miyake, Makito
author_facet Miyake, Makito
Ross, Shanti
Lawton, Adrienne
Chang, Myron
Dai, Yunfeng
Mengual Brichs, Lourdes
Alcaraz Asensio, Antonio
Giacoia, Eva Gomes
Goodison, Steve
Rosser, Charles J.
author_role author
author2 Ross, Shanti
Lawton, Adrienne
Chang, Myron
Dai, Yunfeng
Mengual Brichs, Lourdes
Alcaraz Asensio, Antonio
Giacoia, Eva Gomes
Goodison, Steve
Rosser, Charles J.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de bufeta
Marcadors tumorals
Assaigs clínics
Orina
Bladder cancer
Tumor markers
Clinical trials
Urine
topic Càncer de bufeta
Marcadors tumorals
Assaigs clínics
Orina
Bladder cancer
Tumor markers
Clinical trials
Urine
description BACKGROUND: In this study, we further investigated the association of two biomarkers, CCL18 and A1AT, with bladder cancer (BCa) and evaluated the influence of potentially confounding factors in an experimental model. METHODS: In a cohort of 308 subjects (102 with BCa), urinary concentrations of CCL18 and A1AT were assessed by enzyme-linked immunosorbent assay (ELISA). In an experimental model, benign or cancerous cells, in addition to blood, were added to urines from healthy controls and analyzed by ELISA. Lastly, immunohistochemical staining for CCL18 and A1AT in human bladder tumors was performed. RESULTS: Median urinary protein concentrations of CCL18 (52.84 pg/ml vs. 11.13 pg/ml, p < 0.0001) and A1AT (606.4 ng/ml vs. 120.0 ng/ml, p < 0.0001) were significantly elevated in BCa subjects compared to controls. Furthermore, the addition of whole blood to pooled normal urine resulted in a significant increase in both CCL18 and A1AT. IHC staining of bladder tumors revealed CCL18 immunoreactivity in inflammatory cells only, and there was no significant increase in these immunoreactive cells within benign and cancerous tissue and no association with BCa grade nor stage was noted. A1AT immunoreactivity was observed in the cytoplasm of epithelia cells and intensity of immunostaining increased with tumor grade, but not tumor stage. CONCLUSIONS: Further development of A1AT as a diagnostic biomarker for BCa is warranted.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/61144
url https://hdl.handle.net/2445/61144
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1471-2490-13-42
BMC Urology , 2013, vol. 13, num. 1, p. 42
http://dx.doi.org/10.1186/1471-2490-13-42
dc.rights.none.fl_str_mv cc-by (c) Miyake, M. et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Miyake, M. et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411853131579392
score 15.812429

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