Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation

Problematic fermentations frequently drive economic losses and logistic problems in the winemaking industry. Previous studies have determined thermal conditions leading to problematic fermentations, selecting two contrasting yeast strains for further transcriptomic analysis. Saccharomyces cerevisiae...

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Autores: Lerena, María Cecilia, Vargas-Trinidad, Andrea Susana, del-Real, Javier Alonso, Rojo, Maria Cecilia, González, Magalí Lucía, Mercado, Laura Analía, Lijavetzky, Diego Claudio, Querol, Amparo, Combina, Mariana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/307939
Acceso en línea:http://hdl.handle.net/10261/307939
https://api.elsevier.com/content/abstract/scopus_id/85151124956
Access Level:acceso abierto
Palabra clave:Heat shock
qPCR
Saccharomyces cerevisiae
Sluggish fermentations
Wine
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spelling Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic FermentationLerena, María CeciliaVargas-Trinidad, Andrea Susanadel-Real, Javier AlonsoRojo, Maria CeciliaGonzález, Magalí LucíaMercado, Laura AnalíaLijavetzky, Diego ClaudioQuerol, AmparoCombina, MarianaHeat shockqPCRSaccharomyces cerevisiaeSluggish fermentationsWineProblematic fermentations frequently drive economic losses and logistic problems in the winemaking industry. Previous studies have determined thermal conditions leading to problematic fermentations, selecting two contrasting yeast strains for further transcriptomic analysis. Saccharomyces cerevisiae SBB11 showed strong thermosensitivity towards heat shock, while S. cerevisiae PDM was found to be thermotolerant. The aim of this study was to select genes with significantly upregulated expression to be later used as biomarkers for early detection of sluggish fermentation associated with heat shock. Candidate genes were selected from previously obtained RNA-seq data. Alcoholic fermentations were conducted with 4 S. cerevisiae strains SBB11, PDM, M2 and ICV D21. Heat shocks on day 3 of alcoholic fermentation were applied at 36 and 40 °C for 16 h. S. cerevisiae cells were collected at different times after heat shock onset for qPCR analysis of candidate gene expression over time. Three genes showed promising results; SSA1, MGA1 and OPI10 significantly increased expression with respect to the control. The selected genes showed increased expression during the first 9 h post heat shock and are proposed for early detection of sluggish fermentations associated with heat shock.This research was funded by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) through the projects PIP 2014-2016 (Ref 11220130100694) (Argentina), PICT 2016-3406 FONCyT (Argentina) and PICT 2020-2260 FONCYT (Argentina); Instituto Nacional de Tecnología Agropecuaria INTA-PD-E6-I116; and Consejo Superior de Investigaciones Científicas (CSIC) I-COOP B 20316 (Spain).Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Instituto Nacional de Tecnología Agropecuaria (Argentina)Consejo Superior de Investigaciones Científicas (España)0000-0002-6474-90400000-0003-4041-34000000-0001-6464-556X0000-0003-4207-30670000-0002-0798-1564Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/307939https://api.elsevier.com/content/abstract/scopus_id/85151124956reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFermentationhttps://doi.org/10.3390/fermentation9030313Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3079392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
title Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
spellingShingle Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
Lerena, María Cecilia
Heat shock
qPCR
Saccharomyces cerevisiae
Sluggish fermentations
Wine
title_short Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
title_full Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
title_fullStr Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
title_full_unstemmed Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
title_sort Identification of Molecular Markers for Early Detection of Sluggish Fermentation Associated with Heat Shock during Alcoholic Fermentation
dc.creator.none.fl_str_mv Lerena, María Cecilia
Vargas-Trinidad, Andrea Susana
del-Real, Javier Alonso
Rojo, Maria Cecilia
González, Magalí Lucía
Mercado, Laura Analía
Lijavetzky, Diego Claudio
Querol, Amparo
Combina, Mariana
author Lerena, María Cecilia
author_facet Lerena, María Cecilia
Vargas-Trinidad, Andrea Susana
del-Real, Javier Alonso
Rojo, Maria Cecilia
González, Magalí Lucía
Mercado, Laura Analía
Lijavetzky, Diego Claudio
Querol, Amparo
Combina, Mariana
author_role author
author2 Vargas-Trinidad, Andrea Susana
del-Real, Javier Alonso
Rojo, Maria Cecilia
González, Magalí Lucía
Mercado, Laura Analía
Lijavetzky, Diego Claudio
Querol, Amparo
Combina, Mariana
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
Instituto Nacional de Tecnología Agropecuaria (Argentina)
Consejo Superior de Investigaciones Científicas (España)
0000-0002-6474-9040
0000-0003-4041-3400
0000-0001-6464-556X
0000-0003-4207-3067
0000-0002-0798-1564
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Heat shock
qPCR
Saccharomyces cerevisiae
Sluggish fermentations
Wine
topic Heat shock
qPCR
Saccharomyces cerevisiae
Sluggish fermentations
Wine
description Problematic fermentations frequently drive economic losses and logistic problems in the winemaking industry. Previous studies have determined thermal conditions leading to problematic fermentations, selecting two contrasting yeast strains for further transcriptomic analysis. Saccharomyces cerevisiae SBB11 showed strong thermosensitivity towards heat shock, while S. cerevisiae PDM was found to be thermotolerant. The aim of this study was to select genes with significantly upregulated expression to be later used as biomarkers for early detection of sluggish fermentation associated with heat shock. Candidate genes were selected from previously obtained RNA-seq data. Alcoholic fermentations were conducted with 4 S. cerevisiae strains SBB11, PDM, M2 and ICV D21. Heat shocks on day 3 of alcoholic fermentation were applied at 36 and 40 °C for 16 h. S. cerevisiae cells were collected at different times after heat shock onset for qPCR analysis of candidate gene expression over time. Three genes showed promising results; SSA1, MGA1 and OPI10 significantly increased expression with respect to the control. The selected genes showed increased expression during the first 9 h post heat shock and are proposed for early detection of sluggish fermentations associated with heat shock.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/307939
https://api.elsevier.com/content/abstract/scopus_id/85151124956
url http://hdl.handle.net/10261/307939
https://api.elsevier.com/content/abstract/scopus_id/85151124956
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Fermentation
https://doi.org/10.3390/fermentation9030313
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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