Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis

© 2020 by the authors.

Detalles Bibliográficos
Autores: Martínez-Santiago, María Teresa, Viéitez Madriñán, Francisco Javier, Solla, Alejandro, Tapias, Raúl, Ramírez-Martín, Noelia, Corredoira, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/218432
Acceso en línea:http://hdl.handle.net/10261/218432
Access Level:acceso abierto
Palabra clave:Axillary shoot proliferation
Clonal propagation
Micropropagation
Phytophthora cinnamomi
Quercus ilex
Silver thiosulphate
Somatic embryogenesis
Disease-tolerant plants
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spelling Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic EmbryogenesisMartínez-Santiago, María TeresaViéitez Madriñán, Francisco JavierSolla, AlejandroTapias, RaúlRamírez-Martín, NoeliaCorredoira, ElenaAxillary shoot proliferationClonal propagationMicropropagationPhytophthora cinnamomiQuercus ilexSilver thiosulphateSomatic embryogenesisDisease-tolerant plants© 2020 by the authors.Holm oak (Quercus ilex) is one of the most widely distributed tree species in the Mediterranean basin. High mortality rates have been observed in holm oak populations in the southwest of the Iberian Peninsula as a result of oak decline syndrome. Selection and propagation of genotypes tolerant to this syndrome could aid the restoration of affected areas. In this article, we report micropropagation and conservation procedures based on axillary budding and somatic embryogenesis (SE) of holm oak plants, selected for their tolerance to Phytophthora cinnamomi—the main biotic factor responsible for oak decline. Forced shoots were obtained from potted plants of eight different genotypes, and used as stock material to establish in vitro shoot proliferation cultures. Reliable shoot proliferation was obtained in seven out the eight genotypes established in vitro, whereas multiplication rates were genotype-dependent. The highest rooting rates were obtained by culturing shoots for 24 h or 48 h on rooting induction medium containing 25 mg L−1 indole-3-butyric acid, followed by transfer to medium supplemented with 20 µM silver thiosulphate. Axillary shoot cultures can be successful conserved by cold storage for 12 months at 4 °C under dim lighting. Shoot tips, excised from axillary shoot cultures established from tolerant plants, were used as initial explants to induce SE. Somatic embryos and/or nodular embryogenic structures were obtained on induction medium with or without indole-acetic acid 4 mg L−1, in two out the three genotypes evaluated, and induction rates ranged between 2 and 4%. Plantlet recovery was 45% after two months cold stratification of somatic embryos and eight weeks of culture on germination medium. Vegetative propagation of P. cinnamomi-tolerant Q. ilex trees is a valuable milestone towards the restoration of disease-affected areas.This research was partly funded by Xunta de Galicia (Spain) through the project Contrato Programa 2018–2019. Selection of UHu genotypes was funded by the Andalusian Regional Ministry of Science, Innovation and Busi-ness (project P07-RNM-03108), the European Social Fund and European Regional Development Fund.Peer reviewedMultidisciplinary Digital Publishing InstituteXunta de GaliciaJunta de AndalucíaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/218432reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/f11080841Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2184322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
title Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
spellingShingle Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
Martínez-Santiago, María Teresa
Axillary shoot proliferation
Clonal propagation
Micropropagation
Phytophthora cinnamomi
Quercus ilex
Silver thiosulphate
Somatic embryogenesis
Disease-tolerant plants
title_short Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
title_full Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
title_fullStr Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
title_full_unstemmed Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
title_sort Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis
dc.creator.none.fl_str_mv Martínez-Santiago, María Teresa
Viéitez Madriñán, Francisco Javier
Solla, Alejandro
Tapias, Raúl
Ramírez-Martín, Noelia
Corredoira, Elena
author Martínez-Santiago, María Teresa
author_facet Martínez-Santiago, María Teresa
Viéitez Madriñán, Francisco Javier
Solla, Alejandro
Tapias, Raúl
Ramírez-Martín, Noelia
Corredoira, Elena
author_role author
author2 Viéitez Madriñán, Francisco Javier
Solla, Alejandro
Tapias, Raúl
Ramírez-Martín, Noelia
Corredoira, Elena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Xunta de Galicia
Junta de Andalucía
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Axillary shoot proliferation
Clonal propagation
Micropropagation
Phytophthora cinnamomi
Quercus ilex
Silver thiosulphate
Somatic embryogenesis
Disease-tolerant plants
topic Axillary shoot proliferation
Clonal propagation
Micropropagation
Phytophthora cinnamomi
Quercus ilex
Silver thiosulphate
Somatic embryogenesis
Disease-tolerant plants
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/218432
url http://hdl.handle.net/10261/218432
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/f11080841
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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